Optimization of ultrasound-assisted extraction of pectinase enzyme from guava (Psidium guajava) peel: enzyme recovery, specific activity, temperature, and storage stability

This study aimed to investigate the effects of the ultrasound-assisted extraction conditions on the yield, specific activity, temperature, and storage stability of the pectinase enzyme from guava peel. The ultrasound variables studied were sonication time (10-30min), ultrasound temperature (30-50 de...

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Bibliographic Details
Main Authors: Amid, Mehrnoush, Murshid, Fara Syazana, Manap, Mohd Yazid, Sarker, Md. Zaidul Islam
Format: Article
Language:English
English
English
Published: Taylor and Francis Ltd. 2016
Subjects:
Online Access:http://irep.iium.edu.my/46544/
http://irep.iium.edu.my/46544/
http://irep.iium.edu.my/46544/
http://irep.iium.edu.my/46544/1/46544_Optimization%20of%20ultrasound-assisted%20extraction.pdf
http://irep.iium.edu.my/46544/2/55692_Duplex%20real-time%20PCR%20assay%20using%20SYBR_SCOPUS.pdf
http://irep.iium.edu.my/46544/3/55692_Duplex%20real-time%20PCR%20assay%20using%20SYBR_WOS.pdf
Description
Summary:This study aimed to investigate the effects of the ultrasound-assisted extraction conditions on the yield, specific activity, temperature, and storage stability of the pectinase enzyme from guava peel. The ultrasound variables studied were sonication time (10-30min), ultrasound temperature (30-50 degrees C), pH (2.0-8.0), and solvent-to-sample ratio (2:1mL/g to 6:1mL/g). The main goal was to optimize the ultrasound-assisted extraction conditions to maximize the recovery of pectinase from guava peel with the most desirable enzyme-specific activity and stability. Under the optimum conditions, a high yield (96.2%), good specific activity (18.2U/mg), temperature stability (88.3%), and storage stability (90.3%) of the extracted enzyme were achieved. The optimal conditions were 20min sonication time, 40 degrees C temperature, at pH 5.0, using a 4:1mL/g solvent-to-sample ratio. The study demonstrated that optimization of ultrasound-assisted process conditions for the enzyme extraction could improve the enzymatic characteristics and yield of the enzyme.