Chemical analysis and biological properties of two different formulations of white portland cements

White Portland cement (WPC) has generated research interests in the field of endodontics. This study compared between the properties of two formulations of white Portland cement (WPC) of different origin (Malaysia [MA] and Egypt [EG]). WPCs with and without calcium chloride dihydrate were prepared....

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Main Authors:	Aly Ahmed, Hany Mohammed, Luddin, Norhayati, Kannan, Thirumulu Ponnuraj, Mokhtar@Makhtar, Khairani Idah, Ahmad, Azlina
Format:	Article
Language:	English English
Published:	John Wiley and Sons Inc. 2016
Subjects:	RK Dentistry
Online Access:	http://irep.iium.edu.my/46590/ http://irep.iium.edu.my/46590/ http://irep.iium.edu.my/46590/ http://irep.iium.edu.my/46590/1/46590_Chemical%20analysis%20and%20biologica.pdf http://irep.iium.edu.my/46590/2/46590_Chemical%20analysis%20and%20biologica_SCOPUS.pdf

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spelling	iium-465902017-04-05T02:02:06Z http://irep.iium.edu.my/46590/ Chemical analysis and biological properties of two different formulations of white portland cements Aly Ahmed, Hany Mohammed Luddin, Norhayati Kannan, Thirumulu Ponnuraj Mokhtar@Makhtar, Khairani Idah Ahmad, Azlina RK Dentistry White Portland cement (WPC) has generated research interests in the field of endodontics. This study compared between the properties of two formulations of white Portland cement (WPC) of different origin (Malaysia [MA] and Egypt [EG]). WPCs with and without calcium chloride dihydrate were prepared. Scanning electron microscope (SEM), energy dispersive X-ray micro-analysis, and X-ray diffraction were used for surface morphology evaluation, elemental, and phase analysis, respectively. After the preparation of optimized serial dilutions, the cytotoxicity was evaluated on human periodontal ligament fibroblasts (HPLFs) and dental pulp stem cells (DPSCs) using methyl-thiazol-diphenyltetrazolium assay after 24 and 72 h. Cell attachment properties were examined under SEM after 24 and 72 h. Results showed that the surface morphology and chemical composition of both formulations demonstrated detectable variations. The cytotoxicity evaluation showed different cellular responses of HPLFs compared to DSPCs. Both formulations favored the viability of HPLFs. However, the fast set formulations demonstrated severe cytotoxicity on DPSCs. Significant differences between EGWPC and MAWPC were identified (p < 0.05). The cell attachment properties were favorable; however, HPLFs attached and spread over the samples better than DPSCs. In conclusion, WPC of different origin may show differences in chemical and biological properties. The addition of CaCl2 ·2H2 O to WPC can affect its properties. Human cell types may react differently towards different formulations of WPCs. SCANNING 9999:1-14, 2015. © 2015 Wiley Periodicals, Inc. John Wiley and Sons Inc. 2016-07-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/46590/1/46590_Chemical%20analysis%20and%20biologica.pdf application/pdf en http://irep.iium.edu.my/46590/2/46590_Chemical%20analysis%20and%20biologica_SCOPUS.pdf Aly Ahmed, Hany Mohammed and Luddin, Norhayati and Kannan, Thirumulu Ponnuraj and Mokhtar@Makhtar, Khairani Idah and Ahmad, Azlina (2016) Chemical analysis and biological properties of two different formulations of white portland cements. Scanning, 38 (4). pp. 303-316. ISSN 0161-0457 http://onlinelibrary.wiley.com/doi/10.1002/sca.21270/full 10.10002/sca.21270
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RK Dentistry
spellingShingle	RK Dentistry Aly Ahmed, Hany Mohammed Luddin, Norhayati Kannan, Thirumulu Ponnuraj Mokhtar@Makhtar, Khairani Idah Ahmad, Azlina Chemical analysis and biological properties of two different formulations of white portland cements
description	White Portland cement (WPC) has generated research interests in the field of endodontics. This study compared between the properties of two formulations of white Portland cement (WPC) of different origin (Malaysia [MA] and Egypt [EG]). WPCs with and without calcium chloride dihydrate were prepared. Scanning electron microscope (SEM), energy dispersive X-ray micro-analysis, and X-ray diffraction were used for surface morphology evaluation, elemental, and phase analysis, respectively. After the preparation of optimized serial dilutions, the cytotoxicity was evaluated on human periodontal ligament fibroblasts (HPLFs) and dental pulp stem cells (DPSCs) using methyl-thiazol-diphenyltetrazolium assay after 24 and 72 h. Cell attachment properties were examined under SEM after 24 and 72 h. Results showed that the surface morphology and chemical composition of both formulations demonstrated detectable variations. The cytotoxicity evaluation showed different cellular responses of HPLFs compared to DSPCs. Both formulations favored the viability of HPLFs. However, the fast set formulations demonstrated severe cytotoxicity on DPSCs. Significant differences between EGWPC and MAWPC were identified (p < 0.05). The cell attachment properties were favorable; however, HPLFs attached and spread over the samples better than DPSCs. In conclusion, WPC of different origin may show differences in chemical and biological properties. The addition of CaCl2 ·2H2 O to WPC can affect its properties. Human cell types may react differently towards different formulations of WPCs. SCANNING 9999:1-14, 2015. © 2015 Wiley Periodicals, Inc.
format	Article
author	Aly Ahmed, Hany Mohammed Luddin, Norhayati Kannan, Thirumulu Ponnuraj Mokhtar@Makhtar, Khairani Idah Ahmad, Azlina
author_facet	Aly Ahmed, Hany Mohammed Luddin, Norhayati Kannan, Thirumulu Ponnuraj Mokhtar@Makhtar, Khairani Idah Ahmad, Azlina
author_sort	Aly Ahmed, Hany Mohammed
title	Chemical analysis and biological properties of two different formulations of white portland cements
title_short	Chemical analysis and biological properties of two different formulations of white portland cements
title_full	Chemical analysis and biological properties of two different formulations of white portland cements
title_fullStr	Chemical analysis and biological properties of two different formulations of white portland cements
title_full_unstemmed	Chemical analysis and biological properties of two different formulations of white portland cements
title_sort	chemical analysis and biological properties of two different formulations of white portland cements
publisher	John Wiley and Sons Inc.
publishDate	2016
url	http://irep.iium.edu.my/46590/ http://irep.iium.edu.my/46590/ http://irep.iium.edu.my/46590/ http://irep.iium.edu.my/46590/1/46590_Chemical%20analysis%20and%20biologica.pdf http://irep.iium.edu.my/46590/2/46590_Chemical%20analysis%20and%20biologica_SCOPUS.pdf
first_indexed	2023-09-18T21:06:20Z
last_indexed	2023-09-18T21:06:20Z
_version_	1777410957820035072

Chemical analysis and biological properties of two different formulations of white portland cements

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