Development and validation of bioanalytical method for amlodipine in human plasma with optimised extraction method using Design of Experiment (DOE)

Amlodipine is antihypertensive drug belonging to calcium antagonist group. This study aims to apply experimental design for the optimization of extraction method of amlodipine from human plasma for higher and constant recovery. A chromatographic method involving HPLC (High Performance Liquid Chroma...

Full description

Bibliographic Details
Main Authors:	Helaluddin , Abul Bashar Mohammed, Alaama, Mohammed, Mohamad , Huda Jamilah, Amirudin, N. S.
Format:	Conference or Workshop Item
Language:	English
Published:	2015
Subjects:	QD Chemistry RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/46618/ http://irep.iium.edu.my/46618/ http://irep.iium.edu.my/46618/1/IPNaCS_2015_%28Development_and_validation_of_bioanalytical_method_for_amlodipine_in_human_plasma_with_optimised_extraction_method_using_DOE.pdf

id	iium-46618
recordtype	eprints
spelling	iium-466182016-03-03T00:43:25Z http://irep.iium.edu.my/46618/ Development and validation of bioanalytical method for amlodipine in human plasma with optimised extraction method using Design of Experiment (DOE) Helaluddin , Abul Bashar Mohammed Alaama, Mohammed Mohamad , Huda Jamilah Amirudin, N. S. QD Chemistry RS Pharmacy and materia medica Amlodipine is antihypertensive drug belonging to calcium antagonist group. This study aims to apply experimental design for the optimization of extraction method of amlodipine from human plasma for higher and constant recovery. A chromatographic method involving HPLC (High Performance Liquid Chromatography) with DAD (Diode Array Detector) was developed and validated for the determination of Amlodipine in human plasma with cetirizine as internal standard. Sample preparation for the extraction of Amlodipine and Cetirizine (Internal Standard) from plasma was optimised using DOE (Design of Experiment). Three different parameters namely solvent type, solvent volume and pH were monitored at different level for the optimisation of sample preparation that would produce best recovery of the analytes. For this purpose, three level full factorial design, which involves 27 experiments, was conducted. The recovery of amlodipine was determined using the developed and pre validated HPLC-DAD method with an Agilent 1100 HPLC system. Chromatographic separation of the analytes was obtained using phenomenix (150X4.5 mm, 5 um) C18 column. The mobile phase consists of 35:65 v/v of ACN: 0.3% triethylamine with pH adjusted at 3 using phosphoric acid. Detection of analyte was conducted using a diod array detector at 237 nm wavelength. Design Expert 8.0 software was used to interpret all data obtained from the optimisation study. For the parameters solvent and solvent volume “acetonitrile” and “1 ml” was found to be the optimum values required to extract amlodipine and internal standard while pH had no effect on the optimum recovery. The recovery of amlodipine and internal standard was 80-88% and 85-99% respectively. 2015 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/46618/1/IPNaCS_2015_%28Development_and_validation_of_bioanalytical_method_for_amlodipine_in_human_plasma_with_optimised_extraction_method_using_DOE.pdf Helaluddin , Abul Bashar Mohammed and Alaama, Mohammed and Mohamad , Huda Jamilah and Amirudin, N. S. (2015) Development and validation of bioanalytical method for amlodipine in human plasma with optimised extraction method using Design of Experiment (DOE). In: 4th International Conference on Pharmaceuticals, Nutraceuticals and Cosmetic Science (IPNaCs), 12th-13th Nov. 2015, Ramada Plaza, Malaka, Malaysia. http://ipnacs.uitm.edu.my/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry RS Pharmacy and materia medica
spellingShingle	QD Chemistry RS Pharmacy and materia medica Helaluddin , Abul Bashar Mohammed Alaama, Mohammed Mohamad , Huda Jamilah Amirudin, N. S. Development and validation of bioanalytical method for amlodipine in human plasma with optimised extraction method using Design of Experiment (DOE)
description	Amlodipine is antihypertensive drug belonging to calcium antagonist group. This study aims to apply experimental design for the optimization of extraction method of amlodipine from human plasma for higher and constant recovery. A chromatographic method involving HPLC (High Performance Liquid Chromatography) with DAD (Diode Array Detector) was developed and validated for the determination of Amlodipine in human plasma with cetirizine as internal standard. Sample preparation for the extraction of Amlodipine and Cetirizine (Internal Standard) from plasma was optimised using DOE (Design of Experiment). Three different parameters namely solvent type, solvent volume and pH were monitored at different level for the optimisation of sample preparation that would produce best recovery of the analytes. For this purpose, three level full factorial design, which involves 27 experiments, was conducted. The recovery of amlodipine was determined using the developed and pre validated HPLC-DAD method with an Agilent 1100 HPLC system. Chromatographic separation of the analytes was obtained using phenomenix (150X4.5 mm, 5 um) C18 column. The mobile phase consists of 35:65 v/v of ACN: 0.3% triethylamine with pH adjusted at 3 using phosphoric acid. Detection of analyte was conducted using a diod array detector at 237 nm wavelength. Design Expert 8.0 software was used to interpret all data obtained from the optimisation study. For the parameters solvent and solvent volume “acetonitrile” and “1 ml” was found to be the optimum values required to extract amlodipine and internal standard while pH had no effect on the optimum recovery. The recovery of amlodipine and internal standard was 80-88% and 85-99% respectively.
format	Conference or Workshop Item
author	Helaluddin , Abul Bashar Mohammed Alaama, Mohammed Mohamad , Huda Jamilah Amirudin, N. S.
author_facet	Helaluddin , Abul Bashar Mohammed Alaama, Mohammed Mohamad , Huda Jamilah Amirudin, N. S.
author_sort	Helaluddin , Abul Bashar Mohammed
title	Development and validation of bioanalytical method for amlodipine in human plasma with optimised extraction method using Design of Experiment (DOE)
title_short	Development and validation of bioanalytical method for amlodipine in human plasma with optimised extraction method using Design of Experiment (DOE)
title_full	Development and validation of bioanalytical method for amlodipine in human plasma with optimised extraction method using Design of Experiment (DOE)
title_fullStr	Development and validation of bioanalytical method for amlodipine in human plasma with optimised extraction method using Design of Experiment (DOE)
title_full_unstemmed	Development and validation of bioanalytical method for amlodipine in human plasma with optimised extraction method using Design of Experiment (DOE)
title_sort	development and validation of bioanalytical method for amlodipine in human plasma with optimised extraction method using design of experiment (doe)
publishDate	2015
url	http://irep.iium.edu.my/46618/ http://irep.iium.edu.my/46618/ http://irep.iium.edu.my/46618/1/IPNaCS_2015_%28Development_and_validation_of_bioanalytical_method_for_amlodipine_in_human_plasma_with_optimised_extraction_method_using_DOE.pdf
first_indexed	2023-09-18T21:06:21Z
last_indexed	2023-09-18T21:06:21Z
_version_	1777410959388704768

Development and validation of bioanalytical method for amlodipine in human plasma with optimised extraction method using Design of Experiment (DOE)

Similar Items