Cytotoxicity studies of lung cancer cells using impedance biosensor

Electrical cell-substrate impedance sensing (ECIS) is a valuable tool for real time monitoring of cell behavior such as attachment, mobility, and growth. To employ ECIS, the cells need to attach, spread and proliferate on the sensor in the presence of adhesion-promoting protein that mimics the extra...

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Main Authors:	Mohd Mansor, Ahmad Fairuzabadi, Nordin, Anis Nurashikin, Ibrahim, Irmanisha
Format:	Conference or Workshop Item
Language:	English English English
Published:	Institute of Electrical and Electronics Engineers Inc. 2015
Subjects:	TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices
Online Access:	http://irep.iium.edu.my/46640/ http://irep.iium.edu.my/46640/ http://irep.iium.edu.my/46640/ http://irep.iium.edu.my/46640/1/46640_Cytotoxicity%20studies%20of%20lung%20cancer%20cells.pdf http://irep.iium.edu.my/46640/2/46640_Cytotoxicity%20studies%20of%20lung%20cancer%20cells_SCOPUS.pdf http://irep.iium.edu.my/46640/3/46640_Cytotoxicity%20studies%20of%20lung%20cancer%20cells_WOS.pdf

id	iium-46640
recordtype	eprints
spelling	iium-466402019-09-05T03:16:14Z http://irep.iium.edu.my/46640/ Cytotoxicity studies of lung cancer cells using impedance biosensor Mohd Mansor, Ahmad Fairuzabadi Nordin, Anis Nurashikin Ibrahim, Irmanisha TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices Electrical cell-substrate impedance sensing (ECIS) is a valuable tool for real time monitoring of cell behavior such as attachment, mobility, and growth. To employ ECIS, the cells need to attach, spread and proliferate on the sensor in the presence of adhesion-promoting protein that mimics the extracellular matrix (ECM) of the cells. For cell attachment, collagen I, Bovine had been used as the coating substrate. In this study, four designs with varying electrode distances had been measured to detect the changes in impedance values of Lung Carcinoma cell lines (A549). The impedance change due to the cell growth and attachment was modeled as an equivalent circuit consisting of resistors and capacitors of both the cell culture media and the cells. The impedance measurements were measured every 8 hours for 120 hours at frequencies of 100Hz to 10MHz using Agilent Precision Impedance Analyzer 4294A. The experimental results have shown that the closest distance of the electrode gave the most optimum impedance value for A549 cancer cell’s measurement. The cancer cells were also treated with a chemotherapeutic drug, Taxol and its impedance response was monitored over 5 days. Experimental results show that there is significant reduction in impedance when the cancer cells were exposed to Taxol, indicating that the cells are no longer adherent to the sensor’s surface or are dead. Institute of Electrical and Electronics Engineers Inc. 2015 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/46640/1/46640_Cytotoxicity%20studies%20of%20lung%20cancer%20cells.pdf application/pdf en http://irep.iium.edu.my/46640/2/46640_Cytotoxicity%20studies%20of%20lung%20cancer%20cells_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/46640/3/46640_Cytotoxicity%20studies%20of%20lung%20cancer%20cells_WOS.pdf Mohd Mansor, Ahmad Fairuzabadi and Nordin, Anis Nurashikin and Ibrahim, Irmanisha (2015) Cytotoxicity studies of lung cancer cells using impedance biosensor. In: 2015 International Conference on Smart Sensors and Application (ICSSA), 26th-28th May 2015, Grand Seasons Hotel, Kuala Lumpur. https://ieeexplore.ieee.org/document/7322499 10.1109/ICSSA.2015.7322499
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices
spellingShingle	TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices Mohd Mansor, Ahmad Fairuzabadi Nordin, Anis Nurashikin Ibrahim, Irmanisha Cytotoxicity studies of lung cancer cells using impedance biosensor
description	Electrical cell-substrate impedance sensing (ECIS) is a valuable tool for real time monitoring of cell behavior such as attachment, mobility, and growth. To employ ECIS, the cells need to attach, spread and proliferate on the sensor in the presence of adhesion-promoting protein that mimics the extracellular matrix (ECM) of the cells. For cell attachment, collagen I, Bovine had been used as the coating substrate. In this study, four designs with varying electrode distances had been measured to detect the changes in impedance values of Lung Carcinoma cell lines (A549). The impedance change due to the cell growth and attachment was modeled as an equivalent circuit consisting of resistors and capacitors of both the cell culture media and the cells. The impedance measurements were measured every 8 hours for 120 hours at frequencies of 100Hz to 10MHz using Agilent Precision Impedance Analyzer 4294A. The experimental results have shown that the closest distance of the electrode gave the most optimum impedance value for A549 cancer cell’s measurement. The cancer cells were also treated with a chemotherapeutic drug, Taxol and its impedance response was monitored over 5 days. Experimental results show that there is significant reduction in impedance when the cancer cells were exposed to Taxol, indicating that the cells are no longer adherent to the sensor’s surface or are dead.
format	Conference or Workshop Item
author	Mohd Mansor, Ahmad Fairuzabadi Nordin, Anis Nurashikin Ibrahim, Irmanisha
author_facet	Mohd Mansor, Ahmad Fairuzabadi Nordin, Anis Nurashikin Ibrahim, Irmanisha
author_sort	Mohd Mansor, Ahmad Fairuzabadi
title	Cytotoxicity studies of lung cancer cells using impedance biosensor
title_short	Cytotoxicity studies of lung cancer cells using impedance biosensor
title_full	Cytotoxicity studies of lung cancer cells using impedance biosensor
title_fullStr	Cytotoxicity studies of lung cancer cells using impedance biosensor
title_full_unstemmed	Cytotoxicity studies of lung cancer cells using impedance biosensor
title_sort	cytotoxicity studies of lung cancer cells using impedance biosensor
publisher	Institute of Electrical and Electronics Engineers Inc.
publishDate	2015
url	http://irep.iium.edu.my/46640/ http://irep.iium.edu.my/46640/ http://irep.iium.edu.my/46640/ http://irep.iium.edu.my/46640/1/46640_Cytotoxicity%20studies%20of%20lung%20cancer%20cells.pdf http://irep.iium.edu.my/46640/2/46640_Cytotoxicity%20studies%20of%20lung%20cancer%20cells_SCOPUS.pdf http://irep.iium.edu.my/46640/3/46640_Cytotoxicity%20studies%20of%20lung%20cancer%20cells_WOS.pdf
first_indexed	2023-09-18T21:06:24Z
last_indexed	2023-09-18T21:06:24Z
_version_	1777410961892704256

Cytotoxicity studies of lung cancer cells using impedance biosensor

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