Screen printed impedance biosensor for cytotoxicity studies of lung carcinoma cells

Electrical Cell-Substrate Impedance Sensing is a powerful tool for monitoring real time cells properties such as adhesion, mobility and cytotoxicity. In this study, a silver/silver chloride screen-printed impedance biosensor was developed to characterize A549 lung carcinoma cells growth in the prese...

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Main Authors:	Mohd Mansor, Ahmad Fairuzabadi, Ibrahim, I, Voiculescu, Ioana, Nordin, Anis Nurashikin
Format:	Conference or Workshop Item
Language:	English English
Published:	Springer Singapore 2016
Subjects:	TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices
Online Access:	http://irep.iium.edu.my/46641/ http://irep.iium.edu.my/46641/ http://irep.iium.edu.my/46641/4/ICIBEL_2015-completed.pdf http://irep.iium.edu.my/46641/7/46641_Screen%20printed%20impedance%20biosensor_scopus.pdf

id	iium-46641
recordtype	eprints
spelling	iium-466412018-03-07T01:33:37Z http://irep.iium.edu.my/46641/ Screen printed impedance biosensor for cytotoxicity studies of lung carcinoma cells Mohd Mansor, Ahmad Fairuzabadi Ibrahim, I Voiculescu, Ioana Nordin, Anis Nurashikin TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices Electrical Cell-Substrate Impedance Sensing is a powerful tool for monitoring real time cells properties such as adhesion, mobility and cytotoxicity. In this study, a silver/silver chloride screen-printed impedance biosensor was developed to characterize A549 lung carcinoma cells growth in the presence of collagen I, Bovine. Collagen acts as an extracellular matrix (ECM) for A549 and promotes cell attachment. The sensor was incorporated with a culture well which was fabricated from polydimethylsiloxane (PDMS). A549 cells were cultured in the chambers and impedance measurements were taken at 12 hours intervals for 120 hours. Cell Index (CI) were calculated from the impedance data and plotted in comparison with growth profile of the cells in T-flasks for validation of the sen- sor’s functionality. A549 cells were also treated with anti- tumor drug; Paclitaxel and its response were monitored over 5 days. Experimental results show significant change in CI dur- ing growth and death after exposure to Taxol, indicating that tumor growth was inhibited in the presence of Taxol. Springer Singapore 2016 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/46641/4/ICIBEL_2015-completed.pdf application/pdf en http://irep.iium.edu.my/46641/7/46641_Screen%20printed%20impedance%20biosensor_scopus.pdf Mohd Mansor, Ahmad Fairuzabadi and Ibrahim, I and Voiculescu, Ioana and Nordin, Anis Nurashikin (2016) Screen printed impedance biosensor for cytotoxicity studies of lung carcinoma cells. In: International Conference for Innovation in Biomedical Engineering and Life Sciences (ICIBEL2015), 6 - 8 December 2015, Putrajaya, Malaysia. http://link.springer.com/chapter/10.1007%2F978-981-10-0266-3_25
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices
spellingShingle	TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices Mohd Mansor, Ahmad Fairuzabadi Ibrahim, I Voiculescu, Ioana Nordin, Anis Nurashikin Screen printed impedance biosensor for cytotoxicity studies of lung carcinoma cells
description	Electrical Cell-Substrate Impedance Sensing is a powerful tool for monitoring real time cells properties such as adhesion, mobility and cytotoxicity. In this study, a silver/silver chloride screen-printed impedance biosensor was developed to characterize A549 lung carcinoma cells growth in the presence of collagen I, Bovine. Collagen acts as an extracellular matrix (ECM) for A549 and promotes cell attachment. The sensor was incorporated with a culture well which was fabricated from polydimethylsiloxane (PDMS). A549 cells were cultured in the chambers and impedance measurements were taken at 12 hours intervals for 120 hours. Cell Index (CI) were calculated from the impedance data and plotted in comparison with growth profile of the cells in T-flasks for validation of the sen- sor’s functionality. A549 cells were also treated with anti- tumor drug; Paclitaxel and its response were monitored over 5 days. Experimental results show significant change in CI dur- ing growth and death after exposure to Taxol, indicating that tumor growth was inhibited in the presence of Taxol.
format	Conference or Workshop Item
author	Mohd Mansor, Ahmad Fairuzabadi Ibrahim, I Voiculescu, Ioana Nordin, Anis Nurashikin
author_facet	Mohd Mansor, Ahmad Fairuzabadi Ibrahim, I Voiculescu, Ioana Nordin, Anis Nurashikin
author_sort	Mohd Mansor, Ahmad Fairuzabadi
title	Screen printed impedance biosensor for cytotoxicity studies of lung carcinoma cells
title_short	Screen printed impedance biosensor for cytotoxicity studies of lung carcinoma cells
title_full	Screen printed impedance biosensor for cytotoxicity studies of lung carcinoma cells
title_fullStr	Screen printed impedance biosensor for cytotoxicity studies of lung carcinoma cells
title_full_unstemmed	Screen printed impedance biosensor for cytotoxicity studies of lung carcinoma cells
title_sort	screen printed impedance biosensor for cytotoxicity studies of lung carcinoma cells
publisher	Springer Singapore
publishDate	2016
url	http://irep.iium.edu.my/46641/ http://irep.iium.edu.my/46641/ http://irep.iium.edu.my/46641/4/ICIBEL_2015-completed.pdf http://irep.iium.edu.my/46641/7/46641_Screen%20printed%20impedance%20biosensor_scopus.pdf
first_indexed	2023-09-18T21:06:24Z
last_indexed	2023-09-18T21:06:24Z
_version_	1777410962031116288

Screen printed impedance biosensor for cytotoxicity studies of lung carcinoma cells

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