Morphological change of shed and the effect of VEGF on amniotic membrane scaffold

Angiogenicity is one of the essential components to enable tissue function. It is important to develop a construct that would help in catering oxygen and nutrient to the engineered tissue area. Thus, this study aims to investigate the attachment, spreading and growth of stem cells from human exfoli...

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Main Authors:	Md Hashim , SN, Yusof , MFH, Alshehadat , SA, Kannan , TP, Suzina , SAH, Mokhtar, Khairani Idah, Azlina , A
Format:	Article
Language:	English
Published:	Universiti Putra Malaysia 2015
Subjects:	RK Dentistry
Online Access:	http://irep.iium.edu.my/46913/ http://irep.iium.edu.my/46913/ http://irep.iium.edu.my/46913/1/2015-MJM-Morphological_Change_of_SHED_and_the_Effect_of_VEGF_on__Amniotic_Membrane_Scaffold_%281%29.pdf

id	iium-46913
recordtype	eprints
spelling	iium-469132017-12-13T10:02:58Z http://irep.iium.edu.my/46913/ Morphological change of shed and the effect of VEGF on amniotic membrane scaffold Md Hashim , SN Yusof , MFH Alshehadat , SA Kannan , TP Suzina , SAH Mokhtar, Khairani Idah Azlina , A RK Dentistry Angiogenicity is one of the essential components to enable tissue function. It is important to develop a construct that would help in catering oxygen and nutrient to the engineered tissue area. Thus, this study aims to investigate the attachment, spreading and growth of stem cells from human exfoliated deciduous teeth (SHED) on human AM (HAM) with or without vascular endothelial growth factor (VEGF) using scanning electron microscope (SEM), and indirectly see the potential of the HAM as a scaffold to promote angiogenic micro-environment. Since day 1, there were continuous changes of the cell morphology until day 28, SHED treated with VEGF seemed to change its shape from fibroblast-like into a round-shape cell, similar structure as an endothelial-like cell. The structures of filopodia-like were also observed on the treated SHED. SHED without VEGF treatment showed only normal morphological growth on HAM. VEGF is a protein produced to stimulate angiogenesis, and is believed to contribute to the morphological changes of SHED seeded on HAM. This indicates that HAM could be used as a scaffold to allow SHED differentiation into endothelial-like cells with the induction of VEGF. Universiti Putra Malaysia 2015-12-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/46913/1/2015-MJM-Morphological_Change_of_SHED_and_the_Effect_of_VEGF_on__Amniotic_Membrane_Scaffold_%281%29.pdf Md Hashim , SN and Yusof , MFH and Alshehadat , SA and Kannan , TP and Suzina , SAH and Mokhtar, Khairani Idah and Azlina , A (2015) Morphological change of shed and the effect of VEGF on amniotic membrane scaffold. Malaysian Journal of Microscopy, 11. pp. 1-6. ISSN 1823-7010 http://www.msm.org.my/index.php/microscopy-journal/317-malaysian-journal-of-microscopy-volume-1
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RK Dentistry
spellingShingle	RK Dentistry Md Hashim , SN Yusof , MFH Alshehadat , SA Kannan , TP Suzina , SAH Mokhtar, Khairani Idah Azlina , A Morphological change of shed and the effect of VEGF on amniotic membrane scaffold
description	Angiogenicity is one of the essential components to enable tissue function. It is important to develop a construct that would help in catering oxygen and nutrient to the engineered tissue area. Thus, this study aims to investigate the attachment, spreading and growth of stem cells from human exfoliated deciduous teeth (SHED) on human AM (HAM) with or without vascular endothelial growth factor (VEGF) using scanning electron microscope (SEM), and indirectly see the potential of the HAM as a scaffold to promote angiogenic micro-environment. Since day 1, there were continuous changes of the cell morphology until day 28, SHED treated with VEGF seemed to change its shape from fibroblast-like into a round-shape cell, similar structure as an endothelial-like cell. The structures of filopodia-like were also observed on the treated SHED. SHED without VEGF treatment showed only normal morphological growth on HAM. VEGF is a protein produced to stimulate angiogenesis, and is believed to contribute to the morphological changes of SHED seeded on HAM. This indicates that HAM could be used as a scaffold to allow SHED differentiation into endothelial-like cells with the induction of VEGF.
format	Article
author	Md Hashim , SN Yusof , MFH Alshehadat , SA Kannan , TP Suzina , SAH Mokhtar, Khairani Idah Azlina , A
author_facet	Md Hashim , SN Yusof , MFH Alshehadat , SA Kannan , TP Suzina , SAH Mokhtar, Khairani Idah Azlina , A
author_sort	Md Hashim , SN
title	Morphological change of shed and the effect of VEGF on amniotic membrane scaffold
title_short	Morphological change of shed and the effect of VEGF on amniotic membrane scaffold
title_full	Morphological change of shed and the effect of VEGF on amniotic membrane scaffold
title_fullStr	Morphological change of shed and the effect of VEGF on amniotic membrane scaffold
title_full_unstemmed	Morphological change of shed and the effect of VEGF on amniotic membrane scaffold
title_sort	morphological change of shed and the effect of vegf on amniotic membrane scaffold
publisher	Universiti Putra Malaysia
publishDate	2015
url	http://irep.iium.edu.my/46913/ http://irep.iium.edu.my/46913/ http://irep.iium.edu.my/46913/1/2015-MJM-Morphological_Change_of_SHED_and_the_Effect_of_VEGF_on__Amniotic_Membrane_Scaffold_%281%29.pdf
first_indexed	2023-09-18T21:06:46Z
last_indexed	2023-09-18T21:06:46Z
_version_	1777410985425895424

Morphological change of shed and the effect of VEGF on amniotic membrane scaffold

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