Isolation of thermotolerant bacteria producing fibrinolytic enzyme

Fibrinolytic enzymes were widely used in the treatment of cardiovascular diseases. However, the efficiency of the commercial enzymes are still lack of perfection because there are many side effects as well as not tolerant to downstream processing such as heat sensitive during spray drying process. T...

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Main Authors:	Ab Shukor, Nurul Aqilah, Amid, Azura, Abdul Karim, Mohamed Ismail, Abd Samad, Nadiah Syuhada
Format:	Article
Language:	English English English
Published:	Penerbit UTM 2015
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/47010/ http://irep.iium.edu.my/47010/1/fibronolytic_isolation.pdf http://irep.iium.edu.my/47010/4/47010_Isolation%20of%20thermotolerant%20bacteria_Scopus.pdf http://irep.iium.edu.my/47010/5/47010_Isolation%20of%20thermotolerant%20bacteria_WOS.pdf

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recordtype	eprints
spelling	iium-470102017-09-27T03:27:49Z http://irep.iium.edu.my/47010/ Isolation of thermotolerant bacteria producing fibrinolytic enzyme Ab Shukor, Nurul Aqilah Amid, Azura Abdul Karim, Mohamed Ismail Abd Samad, Nadiah Syuhada TP248.13 Biotechnology Fibrinolytic enzymes were widely used in the treatment of cardiovascular diseases. However, the efficiency of the commercial enzymes are still lack of perfection because there are many side effects as well as not tolerant to downstream processing such as heat sensitive during spray drying process. Therefore, this study presents newly isolated thermophiles bacteria producing fibrinolytic enzyme. Sample was collected from Hot Spring Selayang at Selayang Selangor. Spread plate agar containing skim milk powder growth at pH 7, 53℃ for 24 hours was utilized to isolate thermotolerant bacteria producing protease. Further isolation on bacteria producing fibrinolytic enzyme was carried out using fibrin plate. 16S rDNA gene sequence analysis was used to identify the genotype of the isolates. 27 colonies of thermotolerant bacteria were isolated, however, only 19 of them showing proteolytic activity. All of the 19 isolates are motile and cocci in shapes, with 4 types of arrangement, which are single, diplo (pair), strepto (chain) and staphylo (cluster). HSP04 and HSP11 are gram positive bacteria and others are gram negative. From 19 isolates only 6 were chosen for further analysis. HSP23 showed the highest fibrinolytic activity compared with others. HSP23 was identified as Bacillus licheniformis with 98 % similarity to Bacillus licheniformis DCM 13 and Bacillus licheniformis strain ATCC 14580. Penerbit UTM 2015 Article PeerReviewed application/pdf en http://irep.iium.edu.my/47010/1/fibronolytic_isolation.pdf application/pdf en http://irep.iium.edu.my/47010/4/47010_Isolation%20of%20thermotolerant%20bacteria_Scopus.pdf application/pdf en http://irep.iium.edu.my/47010/5/47010_Isolation%20of%20thermotolerant%20bacteria_WOS.pdf Ab Shukor, Nurul Aqilah and Amid, Azura and Abdul Karim, Mohamed Ismail and Abd Samad, Nadiah Syuhada (2015) Isolation of thermotolerant bacteria producing fibrinolytic enzyme. Jurnal Teknologi, 77 (25). pp. 83-87. ISSN 0127-9696 E-ISSN 2180-3722
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Ab Shukor, Nurul Aqilah Amid, Azura Abdul Karim, Mohamed Ismail Abd Samad, Nadiah Syuhada Isolation of thermotolerant bacteria producing fibrinolytic enzyme
description	Fibrinolytic enzymes were widely used in the treatment of cardiovascular diseases. However, the efficiency of the commercial enzymes are still lack of perfection because there are many side effects as well as not tolerant to downstream processing such as heat sensitive during spray drying process. Therefore, this study presents newly isolated thermophiles bacteria producing fibrinolytic enzyme. Sample was collected from Hot Spring Selayang at Selayang Selangor. Spread plate agar containing skim milk powder growth at pH 7, 53℃ for 24 hours was utilized to isolate thermotolerant bacteria producing protease. Further isolation on bacteria producing fibrinolytic enzyme was carried out using fibrin plate. 16S rDNA gene sequence analysis was used to identify the genotype of the isolates. 27 colonies of thermotolerant bacteria were isolated, however, only 19 of them showing proteolytic activity. All of the 19 isolates are motile and cocci in shapes, with 4 types of arrangement, which are single, diplo (pair), strepto (chain) and staphylo (cluster). HSP04 and HSP11 are gram positive bacteria and others are gram negative. From 19 isolates only 6 were chosen for further analysis. HSP23 showed the highest fibrinolytic activity compared with others. HSP23 was identified as Bacillus licheniformis with 98 % similarity to Bacillus licheniformis DCM 13 and Bacillus licheniformis strain ATCC 14580.
format	Article
author	Ab Shukor, Nurul Aqilah Amid, Azura Abdul Karim, Mohamed Ismail Abd Samad, Nadiah Syuhada
author_facet	Ab Shukor, Nurul Aqilah Amid, Azura Abdul Karim, Mohamed Ismail Abd Samad, Nadiah Syuhada
author_sort	Ab Shukor, Nurul Aqilah
title	Isolation of thermotolerant bacteria producing fibrinolytic enzyme
title_short	Isolation of thermotolerant bacteria producing fibrinolytic enzyme
title_full	Isolation of thermotolerant bacteria producing fibrinolytic enzyme
title_fullStr	Isolation of thermotolerant bacteria producing fibrinolytic enzyme
title_full_unstemmed	Isolation of thermotolerant bacteria producing fibrinolytic enzyme
title_sort	isolation of thermotolerant bacteria producing fibrinolytic enzyme
publisher	Penerbit UTM
publishDate	2015
url	http://irep.iium.edu.my/47010/ http://irep.iium.edu.my/47010/1/fibronolytic_isolation.pdf http://irep.iium.edu.my/47010/4/47010_Isolation%20of%20thermotolerant%20bacteria_Scopus.pdf http://irep.iium.edu.my/47010/5/47010_Isolation%20of%20thermotolerant%20bacteria_WOS.pdf
first_indexed	2023-09-18T21:06:54Z
last_indexed	2023-09-18T21:06:54Z
_version_	1777410993625759744

Isolation of thermotolerant bacteria producing fibrinolytic enzyme

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