Isolation and identification of 3-chloropropionic acid degrading bacterium from marine spong

Synthetic haloalkanoic acids often applied as active components in herbicides are toxic to the environment and harmful to the living organisms. These compounds are widely released to the environment due to their routine use by agricultural activities. In recent years, accumulation of haloalkanoic a...

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Main Authors:	Mohd Sufian, Nurul Hanani, Mohamad, Mohd Azrul Naim, Tengku Abdul Hamid, Tengku Haziyamin, Huyop, Fahrul Zaman, Abdul Hamid, Azzmer Azzar
Format:	Article
Language:	English
Published:	Penerbit UTM Press 2015
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/47029/ http://irep.iium.edu.my/47029/ http://irep.iium.edu.my/47029/1/Mohd._Sufian_et_al._%282015%29.pdf

id	iium-47029
recordtype	eprints
spelling	iium-470292016-07-18T01:46:52Z http://irep.iium.edu.my/47029/ Isolation and identification of 3-chloropropionic acid degrading bacterium from marine spong Mohd Sufian, Nurul Hanani Mohamad, Mohd Azrul Naim Tengku Abdul Hamid, Tengku Haziyamin Huyop, Fahrul Zaman Abdul Hamid, Azzmer Azzar QR Microbiology Synthetic haloalkanoic acids often applied as active components in herbicides are toxic to the environment and harmful to the living organisms. These compounds are widely released to the environment due to their routine use by agricultural activities. In recent years, accumulation of haloalkanoic acids was emerged in marine environment. In this study, a 3CP-degrading bacterium which designated as strain H4 was successfully isolated from marine sponge Gelliodes sp. that capable of degrading 3CP as the sole carbon and energy source. The bacteria growth on solid minimal media containing 3CP was the evident for the presence of dehalogenase enzyme. In liquid medium, the doubling time of the cells for strain H4 was 56.82 ± 0.1 h while the maximum chloride ion release was 2.03 ± 0.01 mM. The 16S rDNA sequence of strain H4 was obtained via 16s rRNA gene analysis (1000 bp) and it was closely related to Bacillus aryabhattai B8W22 (99% similarity). To the best of knowledge, this report is the first report detailing haloalkanoic acid degrading bacteria from marine sponge in coastal area of Malaysia. Penerbit UTM Press 2015-12-20 Article PeerReviewed application/pdf en http://irep.iium.edu.my/47029/1/Mohd._Sufian_et_al._%282015%29.pdf Mohd Sufian, Nurul Hanani and Mohamad, Mohd Azrul Naim and Tengku Abdul Hamid, Tengku Haziyamin and Huyop, Fahrul Zaman and Abdul Hamid, Azzmer Azzar (2015) Isolation and identification of 3-chloropropionic acid degrading bacterium from marine spong. Jurnal Teknologi (Sciences & Engineering) , 77 (25). pp. 71-75. ISSN 0127–9696 E-ISSN 2180–3722 http://www.jurnalteknologi.utm.my/index.php/jurnalteknologi
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Mohd Sufian, Nurul Hanani Mohamad, Mohd Azrul Naim Tengku Abdul Hamid, Tengku Haziyamin Huyop, Fahrul Zaman Abdul Hamid, Azzmer Azzar Isolation and identification of 3-chloropropionic acid degrading bacterium from marine spong
description	Synthetic haloalkanoic acids often applied as active components in herbicides are toxic to the environment and harmful to the living organisms. These compounds are widely released to the environment due to their routine use by agricultural activities. In recent years, accumulation of haloalkanoic acids was emerged in marine environment. In this study, a 3CP-degrading bacterium which designated as strain H4 was successfully isolated from marine sponge Gelliodes sp. that capable of degrading 3CP as the sole carbon and energy source. The bacteria growth on solid minimal media containing 3CP was the evident for the presence of dehalogenase enzyme. In liquid medium, the doubling time of the cells for strain H4 was 56.82 ± 0.1 h while the maximum chloride ion release was 2.03 ± 0.01 mM. The 16S rDNA sequence of strain H4 was obtained via 16s rRNA gene analysis (1000 bp) and it was closely related to Bacillus aryabhattai B8W22 (99% similarity). To the best of knowledge, this report is the first report detailing haloalkanoic acid degrading bacteria from marine sponge in coastal area of Malaysia.
format	Article
author	Mohd Sufian, Nurul Hanani Mohamad, Mohd Azrul Naim Tengku Abdul Hamid, Tengku Haziyamin Huyop, Fahrul Zaman Abdul Hamid, Azzmer Azzar
author_facet	Mohd Sufian, Nurul Hanani Mohamad, Mohd Azrul Naim Tengku Abdul Hamid, Tengku Haziyamin Huyop, Fahrul Zaman Abdul Hamid, Azzmer Azzar
author_sort	Mohd Sufian, Nurul Hanani
title	Isolation and identification of 3-chloropropionic acid degrading bacterium from marine spong
title_short	Isolation and identification of 3-chloropropionic acid degrading bacterium from marine spong
title_full	Isolation and identification of 3-chloropropionic acid degrading bacterium from marine spong
title_fullStr	Isolation and identification of 3-chloropropionic acid degrading bacterium from marine spong
title_full_unstemmed	Isolation and identification of 3-chloropropionic acid degrading bacterium from marine spong
title_sort	isolation and identification of 3-chloropropionic acid degrading bacterium from marine spong
publisher	Penerbit UTM Press
publishDate	2015
url	http://irep.iium.edu.my/47029/ http://irep.iium.edu.my/47029/ http://irep.iium.edu.my/47029/1/Mohd._Sufian_et_al._%282015%29.pdf
first_indexed	2023-09-18T21:06:55Z
last_indexed	2023-09-18T21:06:55Z
_version_	1777410995338084352

Isolation and identification of 3-chloropropionic acid degrading bacterium from marine spong

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