Isolation of moderately halophilic lipase producing bacteria from sponges in Pahang coastal water, Malaysia

Sponges (Porifera) harbour diverse microorganisms which can be the potential source for microbial enzymes such as lipase. In this study, moderately halophilic lipase producing bacteria were isolated from sponges tissues collected near Balok, at Pahang coastal water. Out of 70 isolates that grew on t...

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Main Authors:	Tengku Abdul Hamid, Tengku Haziyamin, Abdul Hamid, Azzmer Azzar, Padzil, Nurul Husna
Format:	Article
Language:	English English English
Published:	Universiti Teknologi Malaysia 2015
Online Access:	http://irep.iium.edu.my/47351/ http://irep.iium.edu.my/47351/ http://irep.iium.edu.my/47351/ http://irep.iium.edu.my/47351/1/6740-18514-1-SM.pdf http://irep.iium.edu.my/47351/4/47351_Isolation%20of%20moderately%20halophilic%20lipase%20producing_Scopus.pdf http://irep.iium.edu.my/47351/9/47351_Isolation%20of%20moderately%20halophilic%20lipase%20producing_WOS.pdf

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spelling	iium-473512017-09-27T03:21:56Z http://irep.iium.edu.my/47351/ Isolation of moderately halophilic lipase producing bacteria from sponges in Pahang coastal water, Malaysia Tengku Abdul Hamid, Tengku Haziyamin Abdul Hamid, Azzmer Azzar Padzil, Nurul Husna Sponges (Porifera) harbour diverse microorganisms which can be the potential source for microbial enzymes such as lipase. In this study, moderately halophilic lipase producing bacteria were isolated from sponges tissues collected near Balok, at Pahang coastal water. Out of 70 isolates that grew on tributyrin agar plate, only 7 isolates had produced clear zones surrounding their colonies. Out of these, 5 isolates appeared to be gram-positive rod; meanwhile, the other 2 isolates were gram-negative rod in morphologies. These isolates were subjected to several biochemical tests i.e., oxidase, gelatin hydrolysis, lactose fermentation, citrate and motility test, and 16S rRNA gene amplification and sequencing. The results from 16S rRNA sequencing showed that 2 isolates (NHTH 6B and NHTH 28A) were highly similar (>97%) with Paenibacillus illinoisensis; isolate NHTH 26A with Stenotrophomonas pavanii; and isolate NHTH 29A with Enterobacter aerogenes. Phylogenetic analysis on selected isolates (NHTH 6B, NHTH 26A, NHTH 28A and NHTH 29A) with other species from the database showed high bootstrap values of above 50%. This showed that diverse phyla of lipase producing bacteria were isolated from the sponge collected from Pahang coastal water. In the isolation of industrial important species, the presence of pathogenic group of microorganism in this sponge could indicate issues on water quality and safety in this area. Universiti Teknologi Malaysia 2015 Article PeerReviewed application/pdf en http://irep.iium.edu.my/47351/1/6740-18514-1-SM.pdf application/pdf en http://irep.iium.edu.my/47351/4/47351_Isolation%20of%20moderately%20halophilic%20lipase%20producing_Scopus.pdf application/pdf en http://irep.iium.edu.my/47351/9/47351_Isolation%20of%20moderately%20halophilic%20lipase%20producing_WOS.pdf Tengku Abdul Hamid, Tengku Haziyamin and Abdul Hamid, Azzmer Azzar and Padzil, Nurul Husna (2015) Isolation of moderately halophilic lipase producing bacteria from sponges in Pahang coastal water, Malaysia. Jurnal Teknologi, 77 (25). pp. 57-61. ISSN 0127–9696 E-ISSN 2180–3722 http://www.jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/6740 http://dx.doi.org/10.11113/jt.v77.6740
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
description	Sponges (Porifera) harbour diverse microorganisms which can be the potential source for microbial enzymes such as lipase. In this study, moderately halophilic lipase producing bacteria were isolated from sponges tissues collected near Balok, at Pahang coastal water. Out of 70 isolates that grew on tributyrin agar plate, only 7 isolates had produced clear zones surrounding their colonies. Out of these, 5 isolates appeared to be gram-positive rod; meanwhile, the other 2 isolates were gram-negative rod in morphologies. These isolates were subjected to several biochemical tests i.e., oxidase, gelatin hydrolysis, lactose fermentation, citrate and motility test, and 16S rRNA gene amplification and sequencing. The results from 16S rRNA sequencing showed that 2 isolates (NHTH 6B and NHTH 28A) were highly similar (>97%) with Paenibacillus illinoisensis; isolate NHTH 26A with Stenotrophomonas pavanii; and isolate NHTH 29A with Enterobacter aerogenes. Phylogenetic analysis on selected isolates (NHTH 6B, NHTH 26A, NHTH 28A and NHTH 29A) with other species from the database showed high bootstrap values of above 50%. This showed that diverse phyla of lipase producing bacteria were isolated from the sponge collected from Pahang coastal water. In the isolation of industrial important species, the presence of pathogenic group of microorganism in this sponge could indicate issues on water quality and safety in this area.
format	Article
author	Tengku Abdul Hamid, Tengku Haziyamin Abdul Hamid, Azzmer Azzar Padzil, Nurul Husna
spellingShingle	Tengku Abdul Hamid, Tengku Haziyamin Abdul Hamid, Azzmer Azzar Padzil, Nurul Husna Isolation of moderately halophilic lipase producing bacteria from sponges in Pahang coastal water, Malaysia
author_facet	Tengku Abdul Hamid, Tengku Haziyamin Abdul Hamid, Azzmer Azzar Padzil, Nurul Husna
author_sort	Tengku Abdul Hamid, Tengku Haziyamin
title	Isolation of moderately halophilic lipase producing bacteria from sponges in Pahang coastal water, Malaysia
title_short	Isolation of moderately halophilic lipase producing bacteria from sponges in Pahang coastal water, Malaysia
title_full	Isolation of moderately halophilic lipase producing bacteria from sponges in Pahang coastal water, Malaysia
title_fullStr	Isolation of moderately halophilic lipase producing bacteria from sponges in Pahang coastal water, Malaysia
title_full_unstemmed	Isolation of moderately halophilic lipase producing bacteria from sponges in Pahang coastal water, Malaysia
title_sort	isolation of moderately halophilic lipase producing bacteria from sponges in pahang coastal water, malaysia
publisher	Universiti Teknologi Malaysia
publishDate	2015
url	http://irep.iium.edu.my/47351/ http://irep.iium.edu.my/47351/ http://irep.iium.edu.my/47351/ http://irep.iium.edu.my/47351/1/6740-18514-1-SM.pdf http://irep.iium.edu.my/47351/4/47351_Isolation%20of%20moderately%20halophilic%20lipase%20producing_Scopus.pdf http://irep.iium.edu.my/47351/9/47351_Isolation%20of%20moderately%20halophilic%20lipase%20producing_WOS.pdf
first_indexed	2023-09-18T21:07:23Z
last_indexed	2023-09-18T21:07:23Z
_version_	1777411024504225792

Isolation of moderately halophilic lipase producing bacteria from sponges in Pahang coastal water, Malaysia

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