DNA barcoding of gamat and timun laut species from Pangkor Island using 16S mitochondrial ribosomal RNA gene
This study aimed to barcode a commercial sea cucumber (Echinodermata: Holothuroidea) or gamat species i.e., Stichopus horrens Selenka, 1867Error! Reference source not found. or dragonfish, and a timun laut species i.e., Holothuria (Mertensiothuria) leucospilota (Brandt, 1835) or white threads fis...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2015
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/47379/ http://irep.iium.edu.my/47379/ http://irep.iium.edu.my/47379/1/43579.pdf |
| Summary: | This study aimed to barcode a commercial sea cucumber (Echinodermata: Holothuroidea) or
gamat species i.e., Stichopus horrens Selenka, 1867Error! Reference source not found. or
dragonfish, and a timun laut species i.e., Holothuria (Mertensiothuria) leucospilota (Brandt,
1835) or white threads fish from Pangkor Island, Perak Darul Ridzuan, Malaysia applying
partial DNA sequencing of non-protein-coding 16S mitochondrial ribosomal RNA gene.
Pangkor Island in the north region of Peninsular Malaysia is well known for its traditional
production industry of air gamat (body fluid extracts) and minyak gamat (lipid extracts). The
phylogenetic analyses using Neighbour-Joining method and Maximum Likelihood method
supported the morphospecies status of H. leucospilota and S. horrens. Despite the fact that more
specimens and different molecular approaches are required to produce better conclusive results,
the current findings gave a better insight of the importance of morphological approach and 16S
mitochondrial rRNA gene sequencing in identification of the two Malaysian sea cucumber
species from Pangkor Island. Furthermore, the gene barcodes can be used as the commercial
species standard or reference samples in determining the species identities of processed sea
cucumbers from Malaysian markets. |
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