16S rDNA barcoding technique for molecular identification of processed sea cucumbers from selected Malaysian markets

Sea cucumber (Phylum Echinodermata: Class Holothuroidea) is renowned in Malaysia as a resource of food and medicine. In food industry, wide-ranging processing, including gutting, boiling, roasting, and subsequent preservation procedures for long storage are frequently needed prior to marketing an...

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Main Authors:	Kamarudin, Kamarul Rahim, Mohamed Rehan, Maryam, Mohd Noor, Hanina, Ramly, Nur Zazarina, Mohamed Rehan, Aisyah
Format:	Conference or Workshop Item
Language:	English
Published:	2015
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/47380/ http://irep.iium.edu.my/47380/ http://irep.iium.edu.my/47380/1/47380.pdf

id	iium-47380
recordtype	eprints
spelling	iium-473802016-07-20T02:01:34Z http://irep.iium.edu.my/47380/ 16S rDNA barcoding technique for molecular identification of processed sea cucumbers from selected Malaysian markets Kamarudin, Kamarul Rahim Mohamed Rehan, Maryam Mohd Noor, Hanina Ramly, Nur Zazarina Mohamed Rehan, Aisyah Q Science (General) Sea cucumber (Phylum Echinodermata: Class Holothuroidea) is renowned in Malaysia as a resource of food and medicine. In food industry, wide-ranging processing, including gutting, boiling, roasting, and subsequent preservation procedures for long storage are frequently needed prior to marketing and consumption of sea cucumbers. In fact, the processes cause deformation of original body shape that leads to difficulties in clear species identification and confirmation of marketed sea cucumbers. Besides, sea cucumber products in Malaysian markets are often intentionally unlabelled or mislabelled. Economic fraud, health hazards, and illegal trade of protected species are potential major consequences of the species substitution. Therefore, this study was conducted to determine species identities of processed sea cucumbers from selected Malaysian markets, i.e. Kuantan, Pahang (East Coast region of Peninsular Malaysia); Langkawi Island, Kedah (the Northern region); Kudat, Sabah (near the northernmost point of Borneo, East Malaysia) and Kota Kinabalu, Sabah (in the northwestern Borneo) in the forms of fresh (nine specimens) and dried (16 specimens) products. Five reference samples were included in the analyses consisting of fresh samples that were morphologically identified as Stichopus horrens (gamat species–specimens) and Holothuria (Mertensiothuria) leucospilota (timun laut species– three specimens) from Pangkor Island, Perak. No species details were tagged to most of the sea cucumber-based food products. Phylogenetic analyses of 30 partial sequences of non-protein-coding 16S mitochondrial ribosomal RNA (rRNA) gene using five main methods, i.e. Neighbour-Joining (NJ), Maximum Likelihood (ML), Minimum Evolution (ME), Unweighted Pair Group Method with Arithmetic Mean (UPGMA), and Maximum Parsimony (MP) showed the presence of two main families of sea cucumbers: Stichopodidae (gamat family) and Holothuriidae (timun laut family), and hence the Basic Local Alignment Search Tool (BLAST) results were supported. This study recorded seven sea cucumber species in total. Specimens of S. horrens, Stichopus herrmanni and Thelenota anax were the three gamat species that clustered under family Stichopodidae. Meanwhile, specimens of H. leucospilota, Holothuria (Halodeima) edulis, Holothuria (Metriatyla) scabra and H. scabra var. versicolor were the four timun laut species that clustered under family Holothuriidae. 2015-10-10 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/47380/1/47380.pdf Kamarudin, Kamarul Rahim and Mohamed Rehan, Maryam and Mohd Noor, Hanina and Ramly, Nur Zazarina and Mohamed Rehan, Aisyah (2015) 16S rDNA barcoding technique for molecular identification of processed sea cucumbers from selected Malaysian markets. In: The 3rd International Postgraduate Conference on Science and Mathematics 2015 (IPCSM’15), 10th-11th Oct. 2015, Sultan Idris Education University (UPSI), Tanjong Malim, Perak Darul Ridzuan, MALAYSIA. http://ipcsm.upsi.edu.my/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Kamarudin, Kamarul Rahim Mohamed Rehan, Maryam Mohd Noor, Hanina Ramly, Nur Zazarina Mohamed Rehan, Aisyah 16S rDNA barcoding technique for molecular identification of processed sea cucumbers from selected Malaysian markets
description	Sea cucumber (Phylum Echinodermata: Class Holothuroidea) is renowned in Malaysia as a resource of food and medicine. In food industry, wide-ranging processing, including gutting, boiling, roasting, and subsequent preservation procedures for long storage are frequently needed prior to marketing and consumption of sea cucumbers. In fact, the processes cause deformation of original body shape that leads to difficulties in clear species identification and confirmation of marketed sea cucumbers. Besides, sea cucumber products in Malaysian markets are often intentionally unlabelled or mislabelled. Economic fraud, health hazards, and illegal trade of protected species are potential major consequences of the species substitution. Therefore, this study was conducted to determine species identities of processed sea cucumbers from selected Malaysian markets, i.e. Kuantan, Pahang (East Coast region of Peninsular Malaysia); Langkawi Island, Kedah (the Northern region); Kudat, Sabah (near the northernmost point of Borneo, East Malaysia) and Kota Kinabalu, Sabah (in the northwestern Borneo) in the forms of fresh (nine specimens) and dried (16 specimens) products. Five reference samples were included in the analyses consisting of fresh samples that were morphologically identified as Stichopus horrens (gamat species–specimens) and Holothuria (Mertensiothuria) leucospilota (timun laut species– three specimens) from Pangkor Island, Perak. No species details were tagged to most of the sea cucumber-based food products. Phylogenetic analyses of 30 partial sequences of non-protein-coding 16S mitochondrial ribosomal RNA (rRNA) gene using five main methods, i.e. Neighbour-Joining (NJ), Maximum Likelihood (ML), Minimum Evolution (ME), Unweighted Pair Group Method with Arithmetic Mean (UPGMA), and Maximum Parsimony (MP) showed the presence of two main families of sea cucumbers: Stichopodidae (gamat family) and Holothuriidae (timun laut family), and hence the Basic Local Alignment Search Tool (BLAST) results were supported. This study recorded seven sea cucumber species in total. Specimens of S. horrens, Stichopus herrmanni and Thelenota anax were the three gamat species that clustered under family Stichopodidae. Meanwhile, specimens of H. leucospilota, Holothuria (Halodeima) edulis, Holothuria (Metriatyla) scabra and H. scabra var. versicolor were the four timun laut species that clustered under family Holothuriidae.
format	Conference or Workshop Item
author	Kamarudin, Kamarul Rahim Mohamed Rehan, Maryam Mohd Noor, Hanina Ramly, Nur Zazarina Mohamed Rehan, Aisyah
author_facet	Kamarudin, Kamarul Rahim Mohamed Rehan, Maryam Mohd Noor, Hanina Ramly, Nur Zazarina Mohamed Rehan, Aisyah
author_sort	Kamarudin, Kamarul Rahim
title	16S rDNA barcoding technique for molecular identification of processed sea cucumbers from selected Malaysian markets
title_short	16S rDNA barcoding technique for molecular identification of processed sea cucumbers from selected Malaysian markets
title_full	16S rDNA barcoding technique for molecular identification of processed sea cucumbers from selected Malaysian markets
title_fullStr	16S rDNA barcoding technique for molecular identification of processed sea cucumbers from selected Malaysian markets
title_full_unstemmed	16S rDNA barcoding technique for molecular identification of processed sea cucumbers from selected Malaysian markets
title_sort	16s rdna barcoding technique for molecular identification of processed sea cucumbers from selected malaysian markets
publishDate	2015
url	http://irep.iium.edu.my/47380/ http://irep.iium.edu.my/47380/ http://irep.iium.edu.my/47380/1/47380.pdf
first_indexed	2023-09-18T21:07:25Z
last_indexed	2023-09-18T21:07:25Z
_version_	1777411026646466560

16S rDNA barcoding technique for molecular identification of processed sea cucumbers from selected Malaysian markets

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