Proliferative and genotoxic effect of Perivitelline fluid from horseshoe crab on human dental pulp stem cells
The aim of this study was to evaluate the effect of perivitelline fluid (PVF) on the proliferation, chromosome aberration and mutagenicity of dental pulp stem cells (DPSCs). Crude extracts were prepared from the PVF collected from horseshoe crabs. For cytotoxicity test (MTT assay), the DPSCs were...
| Main Authors: | , , , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English English |
| Published: |
2015
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/47662/ http://irep.iium.edu.my/47662/ http://irep.iium.edu.my/47662/1/KIM-ISSCR_abstract_2015.pdf http://irep.iium.edu.my/47662/2/ISSCR_poster_Kannan_2015_%281%29_%281%29.pdf |
| Summary: | The aim of this study was to evaluate the effect of perivitelline fluid (PVF) on the
proliferation, chromosome aberration and mutagenicity of dental pulp stem cells
(DPSCs). Crude extracts were prepared from the PVF collected from horseshoe
crabs. For cytotoxicity test (MTT assay), the DPSCs were subjected to different
concentrations of PVF crude extract. Two inhibitory concentrations (IC50=26.887mg/
ml and IC25=14.093mg/ml) and two other concentrations (0.278mg/ml and 0.019mg/
ml) which produced higher cell viability were selected for downstream experiments.
The proliferative effect was assessed using AlamarBlue® assay for a period of 10
days and the population doubling time (PDT) were also calculated based on this
assay. Chromosome aberration and Ames tests were employed for the evaluating
the genotoxicity. Statistical analysis was carried out using independent t-test to
assess the significant differences (p<0.05). AlamarBlue® assay showed that PVF
groups produced comparable proliferation activity to negative (untreated) control.
The PDTs between PVF groups and negative control were also insignificantly
different (p>0.05). Both the chromosome aberration test and Ames test did not show
any significant differences in the PVF treated groups. Hence, it can be concluded
that PVF from horseshoe crabs produced insignificant proliferative activity and was
non-genotoxic on DPSCs based on chromosome aberration and Ames tests.
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