Histopathological changes of the cardiac muscle and the adrenal gland in long term diabetic rat

Diabetes in an animal model is increasingly being used in the investigation of the etiopathogenesis of diabetes and diabetic complications in the long term. Streptozotocin is the chemical used to induce experimental diabetes, mostly in rodents. Administrations of the STZ to adult rats cause insulin...

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Main Authors:	Abdullah, Sinan Mohammed, Abdul Razak, Tariq, Al-Ani, Imad Matloub Dally
Format:	Conference or Workshop Item
Language:	English English
Published:	2014
Subjects:	RM Therapeutics. Pharmacology
Online Access:	http://irep.iium.edu.my/47699/ http://irep.iium.edu.my/47699/ http://irep.iium.edu.my/47699/1/IRIIE_2014_sinan_402.pdf http://irep.iium.edu.my/47699/4/47669.pdf

id	iium-47699
recordtype	eprints
spelling	iium-476992016-03-23T07:08:27Z http://irep.iium.edu.my/47699/ Histopathological changes of the cardiac muscle and the adrenal gland in long term diabetic rat Abdullah, Sinan Mohammed Abdul Razak, Tariq Al-Ani, Imad Matloub Dally RM Therapeutics. Pharmacology Diabetes in an animal model is increasingly being used in the investigation of the etiopathogenesis of diabetes and diabetic complications in the long term. Streptozotocin is the chemical used to induce experimental diabetes, mostly in rodents. Administrations of the STZ to adult rats cause insulin-dependent type 1 diabetes. Cardiovascular diseases as a long-term complication may be found in diabetic experimental animals. These complications are the most significant and commence one of the leading causes of mortality due to diabetes. On the other hand, Diabetes mellitus is associated with abnormal function of the adrenal gland. The aims of this study were to investigate the histopathological changes of cardiac muscle and adrenal gland in diabetic rats. Sixteen adult female Sprague–Dawley rats weighing 200–225g at testing were used. All animals were housed in groups of 2-3 in a temperature (22±1 ºc) and relative-humidity (60–70%) controlled room on a 12:12-h light/dark cycle and allowed free access to water and normal diet for rodents. Animal care complied with that stipulated by the local ethics committee. The rats were randomly divided into 2 groups of 8 animals. Streptozotocin was dissolved in 0.1M Citrate Buffer at pH 4.5 immediately before i.p. Injection at 60mg/kg. Eight rats (diabetic group) received a single i.p. Injection of Streptozotocin (60 mg/kg body weight) following an overnight fast. After six months, animals from control and treated groups (STZ) were sacrificed; dissected and small pieces of the heart and adrenal gland were quickly removed, then fixed in 10% formalin. Following fixation, specimens were dehydrated, embedded, and then sectioned to 4 microns thickness. For histological examinations, sections were stained with Ehrlich Haematoxylin and Eosin. In conclusion, the long-term diabetic rat may act as a useful model to observe the histopathological changes of the cardiac muscle and the adrenal gland in long-term diabetic human. 2014 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/47699/1/IRIIE_2014_sinan_402.pdf application/pdf en http://irep.iium.edu.my/47699/4/47669.pdf Abdullah, Sinan Mohammed and Abdul Razak, Tariq and Al-Ani, Imad Matloub Dally (2014) Histopathological changes of the cardiac muscle and the adrenal gland in long term diabetic rat. In: International Research, Invention and Innovation Exhibition 2014 (IRIIE2014), 11th -13th June 2014, Cultural Activity Center (CAC), Internatioanal Islamic University Malaysia. (Unpublished) http://www.iium.edu.my/irie/14/index.php/result/8-irie2014/36-results-for-has
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RM Therapeutics. Pharmacology
spellingShingle	RM Therapeutics. Pharmacology Abdullah, Sinan Mohammed Abdul Razak, Tariq Al-Ani, Imad Matloub Dally Histopathological changes of the cardiac muscle and the adrenal gland in long term diabetic rat
description	Diabetes in an animal model is increasingly being used in the investigation of the etiopathogenesis of diabetes and diabetic complications in the long term. Streptozotocin is the chemical used to induce experimental diabetes, mostly in rodents. Administrations of the STZ to adult rats cause insulin-dependent type 1 diabetes. Cardiovascular diseases as a long-term complication may be found in diabetic experimental animals. These complications are the most significant and commence one of the leading causes of mortality due to diabetes. On the other hand, Diabetes mellitus is associated with abnormal function of the adrenal gland. The aims of this study were to investigate the histopathological changes of cardiac muscle and adrenal gland in diabetic rats. Sixteen adult female Sprague–Dawley rats weighing 200–225g at testing were used. All animals were housed in groups of 2-3 in a temperature (22±1 ºc) and relative-humidity (60–70%) controlled room on a 12:12-h light/dark cycle and allowed free access to water and normal diet for rodents. Animal care complied with that stipulated by the local ethics committee. The rats were randomly divided into 2 groups of 8 animals. Streptozotocin was dissolved in 0.1M Citrate Buffer at pH 4.5 immediately before i.p. Injection at 60mg/kg. Eight rats (diabetic group) received a single i.p. Injection of Streptozotocin (60 mg/kg body weight) following an overnight fast. After six months, animals from control and treated groups (STZ) were sacrificed; dissected and small pieces of the heart and adrenal gland were quickly removed, then fixed in 10% formalin. Following fixation, specimens were dehydrated, embedded, and then sectioned to 4 microns thickness. For histological examinations, sections were stained with Ehrlich Haematoxylin and Eosin. In conclusion, the long-term diabetic rat may act as a useful model to observe the histopathological changes of the cardiac muscle and the adrenal gland in long-term diabetic human.
format	Conference or Workshop Item
author	Abdullah, Sinan Mohammed Abdul Razak, Tariq Al-Ani, Imad Matloub Dally
author_facet	Abdullah, Sinan Mohammed Abdul Razak, Tariq Al-Ani, Imad Matloub Dally
author_sort	Abdullah, Sinan Mohammed
title	Histopathological changes of the cardiac muscle and the adrenal gland in long term diabetic rat
title_short	Histopathological changes of the cardiac muscle and the adrenal gland in long term diabetic rat
title_full	Histopathological changes of the cardiac muscle and the adrenal gland in long term diabetic rat
title_fullStr	Histopathological changes of the cardiac muscle and the adrenal gland in long term diabetic rat
title_full_unstemmed	Histopathological changes of the cardiac muscle and the adrenal gland in long term diabetic rat
title_sort	histopathological changes of the cardiac muscle and the adrenal gland in long term diabetic rat
publishDate	2014
url	http://irep.iium.edu.my/47699/ http://irep.iium.edu.my/47699/ http://irep.iium.edu.my/47699/1/IRIIE_2014_sinan_402.pdf http://irep.iium.edu.my/47699/4/47669.pdf
first_indexed	2023-09-18T21:07:50Z
last_indexed	2023-09-18T21:07:50Z
_version_	1777411052936364032

Histopathological changes of the cardiac muscle and the adrenal gland in long term diabetic rat

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