Preparation, characterization and in vitro release studies of insulin-loaded double-walled poly(lactide-co-glycolide) microspheres

The purpose of this study was to fabricate insulin-loaded double-walled and singlepolymer poly(lactide-co-glycolide) (PLGA) microspheres using a fast degrading glucose core, hydroxyl-terminated poly(lactide-co-glycolide) (Glu-PLGA) and a moderate degrading carboxyl-terminated PLGA polymers. A modifi...

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Main Authors:	Ansary, Md. Rezaul Haque, Rahman, Md. Mokhlesur, Awang, Mohamed, Katas, Haliza, Ab. Hadi, Hazrina, Abd Almonem, Doolaanea
Format:	Article
Language:	English English
Published:	Springer 2016
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/47774/ http://irep.iium.edu.my/47774/ http://irep.iium.edu.my/47774/ http://irep.iium.edu.my/47774/1/Mokhles-DDTR-D-15-00072_R1.pdf http://irep.iium.edu.my/47774/4/47774_Preparation%2C%20characterization_wos_scopus.pdf

id	iium-47774
recordtype	eprints
spelling	iium-477742017-03-20T04:11:57Z http://irep.iium.edu.my/47774/ Preparation, characterization and in vitro release studies of insulin-loaded double-walled poly(lactide-co-glycolide) microspheres Ansary, Md. Rezaul Haque Rahman, Md. Mokhlesur Awang, Mohamed Katas, Haliza Ab. Hadi, Hazrina Abd Almonem, Doolaanea QD Chemistry The purpose of this study was to fabricate insulin-loaded double-walled and singlepolymer poly(lactide-co-glycolide) (PLGA) microspheres using a fast degrading glucose core, hydroxyl-terminated poly(lactide-co-glycolide) (Glu-PLGA) and a moderate degrading carboxyl-terminated PLGA polymers. A modified water-in-oil-in-oilin-water (w/o/o/w) emulsion solvent evaporation technique was employed to prepare double-walled microspheres, whereas single-polymer microspheres were fabricated by a conventional water-in-oil-in-water (w/o/w) emulsion solvent evaporation method. The effect of fabrication techniques and polymer characteristics on microspheres size,morphology, encapsulation efficiency, in vitro release and insulin stability were evaluated. The prepared double-walled microspheres were essentially non-porous,smooth surfaced, and spherical in shape, whereas single-polymer microspheres were highly porous. Double-walled microspheres exhibited a significantly reduced initial burst followed by sustained and almost complete release of insulin compared to singlepolymer microspheres. Initial burst release was further suppressed from double-walled microspheres when the mass ratio of the component polymers was increased. In conclusion, double-walled microspheres made of Glu-PLGA and PLGA can be a potential delivery system of therapeutic insulin. Springer 2016-04-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/47774/1/Mokhles-DDTR-D-15-00072_R1.pdf application/pdf en http://irep.iium.edu.my/47774/4/47774_Preparation%2C%20characterization_wos_scopus.pdf Ansary, Md. Rezaul Haque and Rahman, Md. Mokhlesur and Awang, Mohamed and Katas, Haliza and Ab. Hadi, Hazrina and Abd Almonem, Doolaanea (2016) Preparation, characterization and in vitro release studies of insulin-loaded double-walled poly(lactide-co-glycolide) microspheres. Drug Delivery and Translational Research. pp. 1-11. ISSN 2190-393X E-ISSN 2190-3948 http://link.springer.com/article/10.1007%2Fs13346-016-0278-y 10.1007/s13346-016-0278-y
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry
spellingShingle	QD Chemistry Ansary, Md. Rezaul Haque Rahman, Md. Mokhlesur Awang, Mohamed Katas, Haliza Ab. Hadi, Hazrina Abd Almonem, Doolaanea Preparation, characterization and in vitro release studies of insulin-loaded double-walled poly(lactide-co-glycolide) microspheres
description	The purpose of this study was to fabricate insulin-loaded double-walled and singlepolymer poly(lactide-co-glycolide) (PLGA) microspheres using a fast degrading glucose core, hydroxyl-terminated poly(lactide-co-glycolide) (Glu-PLGA) and a moderate degrading carboxyl-terminated PLGA polymers. A modified water-in-oil-in-oilin-water (w/o/o/w) emulsion solvent evaporation technique was employed to prepare double-walled microspheres, whereas single-polymer microspheres were fabricated by a conventional water-in-oil-in-water (w/o/w) emulsion solvent evaporation method. The effect of fabrication techniques and polymer characteristics on microspheres size,morphology, encapsulation efficiency, in vitro release and insulin stability were evaluated. The prepared double-walled microspheres were essentially non-porous,smooth surfaced, and spherical in shape, whereas single-polymer microspheres were highly porous. Double-walled microspheres exhibited a significantly reduced initial burst followed by sustained and almost complete release of insulin compared to singlepolymer microspheres. Initial burst release was further suppressed from double-walled microspheres when the mass ratio of the component polymers was increased. In conclusion, double-walled microspheres made of Glu-PLGA and PLGA can be a potential delivery system of therapeutic insulin.
format	Article
author	Ansary, Md. Rezaul Haque Rahman, Md. Mokhlesur Awang, Mohamed Katas, Haliza Ab. Hadi, Hazrina Abd Almonem, Doolaanea
author_facet	Ansary, Md. Rezaul Haque Rahman, Md. Mokhlesur Awang, Mohamed Katas, Haliza Ab. Hadi, Hazrina Abd Almonem, Doolaanea
author_sort	Ansary, Md. Rezaul Haque
title	Preparation, characterization and in vitro release studies of insulin-loaded double-walled poly(lactide-co-glycolide) microspheres
title_short	Preparation, characterization and in vitro release studies of insulin-loaded double-walled poly(lactide-co-glycolide) microspheres
title_full	Preparation, characterization and in vitro release studies of insulin-loaded double-walled poly(lactide-co-glycolide) microspheres
title_fullStr	Preparation, characterization and in vitro release studies of insulin-loaded double-walled poly(lactide-co-glycolide) microspheres
title_full_unstemmed	Preparation, characterization and in vitro release studies of insulin-loaded double-walled poly(lactide-co-glycolide) microspheres
title_sort	preparation, characterization and in vitro release studies of insulin-loaded double-walled poly(lactide-co-glycolide) microspheres
publisher	Springer
publishDate	2016
url	http://irep.iium.edu.my/47774/ http://irep.iium.edu.my/47774/ http://irep.iium.edu.my/47774/ http://irep.iium.edu.my/47774/1/Mokhles-DDTR-D-15-00072_R1.pdf http://irep.iium.edu.my/47774/4/47774_Preparation%2C%20characterization_wos_scopus.pdf
first_indexed	2023-09-18T21:07:56Z
last_indexed	2023-09-18T21:07:56Z
_version_	1777411058710872064

Preparation, characterization and in vitro release studies of insulin-loaded double-walled poly(lactide-co-glycolide) microspheres

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