Optimization of lipase immobilization on nylon -6-grafted with PGMA polymer

Lipase from wheat germ was immobilized by covalent binding on a nylon -6- grafted with PGMA. This polymer was successfully activated with diethyl amine to intact with lipase enzyme molecule. Response surface methodology (RSM) was applied to model and optimize immobilization conditions for maximum...

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Main Authors:	Bahrudin, Nik Adlin, Al-Khatib, Ma An Fahmi Rashid, Nasef, Mohamed Mahmoud
Other Authors:	Haneef, Sayed Sikandar Shah
Format:	Conference or Workshop Item
Language:	English
Published:	Kulliyyah of Engineering, International Islamic University Malaysia 2016
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/47871/ http://irep.iium.edu.my/47871/ http://irep.iium.edu.my/47871/1/47871_Optimization_of_Lipase_Immobilization.pdf

id	iium-47871
recordtype	eprints
spelling	iium-478712017-01-17T01:42:56Z http://irep.iium.edu.my/47871/ Optimization of lipase immobilization on nylon -6-grafted with PGMA polymer Bahrudin, Nik Adlin Al-Khatib, Ma An Fahmi Rashid Nasef, Mohamed Mahmoud TP248.13 Biotechnology Lipase from wheat germ was immobilized by covalent binding on a nylon -6- grafted with PGMA. This polymer was successfully activated with diethyl amine to intact with lipase enzyme molecule. Response surface methodology (RSM) was applied to model and optimize immobilization conditions for maximum activity and understanding the interaction of the factors affecting the activity of the immobilized enzyme. The face centered central composite design (FCCCD) was applied to measure the effect of reaction pH (6-8), reaction time (2-10 h) and enzyme concentration (1-2 mg) on enzyme activity. The result showed that pH, time and enzyme concentration had significant effects on the activity of lipase enzyme. Based on the analysis, the optimum immobilization conditions obtained were at pH 7, immobilization time 5 hours and enzyme concentration 0.90 mg/ml. Kulliyyah of Engineering, International Islamic University Malaysia Haneef, Sayed Sikandar Shah Mohd Zin, Najibah Mahmud , Mek Wok 2016 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/47871/1/47871_Optimization_of_Lipase_Immobilization.pdf Bahrudin, Nik Adlin and Al-Khatib, Ma An Fahmi Rashid and Nasef, Mohamed Mahmoud (2016) Optimization of lipase immobilization on nylon -6-grafted with PGMA polymer. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur. http://www.iium.edu.my/icbioe/2016/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Bahrudin, Nik Adlin Al-Khatib, Ma An Fahmi Rashid Nasef, Mohamed Mahmoud Optimization of lipase immobilization on nylon -6-grafted with PGMA polymer
description	Lipase from wheat germ was immobilized by covalent binding on a nylon -6- grafted with PGMA. This polymer was successfully activated with diethyl amine to intact with lipase enzyme molecule. Response surface methodology (RSM) was applied to model and optimize immobilization conditions for maximum activity and understanding the interaction of the factors affecting the activity of the immobilized enzyme. The face centered central composite design (FCCCD) was applied to measure the effect of reaction pH (6-8), reaction time (2-10 h) and enzyme concentration (1-2 mg) on enzyme activity. The result showed that pH, time and enzyme concentration had significant effects on the activity of lipase enzyme. Based on the analysis, the optimum immobilization conditions obtained were at pH 7, immobilization time 5 hours and enzyme concentration 0.90 mg/ml.
author2	Haneef, Sayed Sikandar Shah
author_facet	Haneef, Sayed Sikandar Shah Bahrudin, Nik Adlin Al-Khatib, Ma An Fahmi Rashid Nasef, Mohamed Mahmoud
format	Conference or Workshop Item
author	Bahrudin, Nik Adlin Al-Khatib, Ma An Fahmi Rashid Nasef, Mohamed Mahmoud
author_sort	Bahrudin, Nik Adlin
title	Optimization of lipase immobilization on nylon -6-grafted with PGMA polymer
title_short	Optimization of lipase immobilization on nylon -6-grafted with PGMA polymer
title_full	Optimization of lipase immobilization on nylon -6-grafted with PGMA polymer
title_fullStr	Optimization of lipase immobilization on nylon -6-grafted with PGMA polymer
title_full_unstemmed	Optimization of lipase immobilization on nylon -6-grafted with PGMA polymer
title_sort	optimization of lipase immobilization on nylon -6-grafted with pgma polymer
publisher	Kulliyyah of Engineering, International Islamic University Malaysia
publishDate	2016
url	http://irep.iium.edu.my/47871/ http://irep.iium.edu.my/47871/ http://irep.iium.edu.my/47871/1/47871_Optimization_of_Lipase_Immobilization.pdf
first_indexed	2023-09-18T21:08:04Z
last_indexed	2023-09-18T21:08:04Z
_version_	1777411067553513472

Optimization of lipase immobilization on nylon -6-grafted with PGMA polymer

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