DNA barcoding of Arius species (Siluriformes: Ariidae) found in Peninsular Malaysia waters, using mitochondrial Cytochrome oxidase subunit I gene.

DNA barcoding of Arius species (Siluriformes: Ariidae) found in Peninsular Malaysia waters, using mitochondrial Cytochrome oxidase subunit I gene.

Ariid catfishes are widely distributed in tropical and temperate continental shelves all over the world, mainly inhabiting inshore waters and estuarine regions, or in some cases confined to freshwaters. Ariid catfishes, commonly known as the sea catfishes, belong to family Ariidae (order: Siluriform...

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Main Authors: Abdurahman, Siti Waznah, Ambak, Mohd Azmi, Md. Sheriff, Shahreza, Chowdhury, Ahmed Jalal Khan
Format: Conference or Workshop Item
Language:English
Published: 2014
Subjects:
QH301 Biology
Online Access:http://irep.iium.edu.my/48139/
http://irep.iium.edu.my/48139/4/48139.pdf
id iium-48139
recordtype eprints
spelling iium-481392018-06-20T04:38:09Z http://irep.iium.edu.my/48139/ DNA barcoding of Arius species (Siluriformes: Ariidae) found in Peninsular Malaysia waters, using mitochondrial Cytochrome oxidase subunit I gene. Abdurahman, Siti Waznah Ambak, Mohd Azmi Md. Sheriff, Shahreza Chowdhury, Ahmed Jalal Khan QH301 Biology Ariid catfishes are widely distributed in tropical and temperate continental shelves all over the world, mainly inhabiting inshore waters and estuarine regions, or in some cases confined to freshwaters. Ariid catfishes, commonly known as the sea catfishes, belong to family Ariidae (order: Siluriformes). Ariids are one of the problematic groups for the identification using their morphological features. Furthermore, keys available for the identification are unclear and confusing. Thus, this study was done in order to determine the species in one of the problematic ariid’s genera, Arius, which found in Peninsular Malaysian waters using molecular approach. There were four species of Arius found in this study, Arius leptonotacanthus, Arius maculatus, Arius oetik and Arius spp. All the species were sequenced for 499-bp region of the mitochondrial cytochrome oxidase subunit I (COI) gene. Five sequences per species were amplified and a neighbour-joining phylogenetic tree using Tamura-3 parameter (T92+G) model was constructed. The results shows that Arius leptonotacanthus formed the base for other Arius species (A.maculatus, A.oetik and Arius spp.) supported by a moderate bootstrap value of 50%. Whereas, Arius maculatus formed a sister taxa to Arius spp. with 91% of bootstrap value. Overall, the phylogenetic tree shows that all the Arius species were grouped in separate clades according to the respective species with the support of high bootstrap values. Thus, this study revealed that this problematic Arius species can be clearly differentiated using the molecular marker. 2014-02-12 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/48139/4/48139.pdf Abdurahman, Siti Waznah and Ambak, Mohd Azmi and Md. Sheriff, Shahreza and Chowdhury, Ahmed Jalal Khan (2014) DNA barcoding of Arius species (Siluriformes: Ariidae) found in Peninsular Malaysia waters, using mitochondrial Cytochrome oxidase subunit I gene. In: Asian Fish Biodiversity Conference 2014 (AFBC 2014), 12th-13th February 2014, Penang, Malaysia. (Unpublished)
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QH301 Biology
spellingShingle QH301 Biology
Abdurahman, Siti Waznah
Ambak, Mohd Azmi
Md. Sheriff, Shahreza
Chowdhury, Ahmed Jalal Khan
DNA barcoding of Arius species (Siluriformes: Ariidae) found in Peninsular Malaysia waters, using mitochondrial Cytochrome oxidase subunit I gene.
description Ariid catfishes are widely distributed in tropical and temperate continental shelves all over the world, mainly inhabiting inshore waters and estuarine regions, or in some cases confined to freshwaters. Ariid catfishes, commonly known as the sea catfishes, belong to family Ariidae (order: Siluriformes). Ariids are one of the problematic groups for the identification using their morphological features. Furthermore, keys available for the identification are unclear and confusing. Thus, this study was done in order to determine the species in one of the problematic ariid’s genera, Arius, which found in Peninsular Malaysian waters using molecular approach. There were four species of Arius found in this study, Arius leptonotacanthus, Arius maculatus, Arius oetik and Arius spp. All the species were sequenced for 499-bp region of the mitochondrial cytochrome oxidase subunit I (COI) gene. Five sequences per species were amplified and a neighbour-joining phylogenetic tree using Tamura-3 parameter (T92+G) model was constructed. The results shows that Arius leptonotacanthus formed the base for other Arius species (A.maculatus, A.oetik and Arius spp.) supported by a moderate bootstrap value of 50%. Whereas, Arius maculatus formed a sister taxa to Arius spp. with 91% of bootstrap value. Overall, the phylogenetic tree shows that all the Arius species were grouped in separate clades according to the respective species with the support of high bootstrap values. Thus, this study revealed that this problematic Arius species can be clearly differentiated using the molecular marker.
format Conference or Workshop Item
author Abdurahman, Siti Waznah
Ambak, Mohd Azmi
Md. Sheriff, Shahreza
Chowdhury, Ahmed Jalal Khan
author_facet Abdurahman, Siti Waznah
Ambak, Mohd Azmi
Md. Sheriff, Shahreza
Chowdhury, Ahmed Jalal Khan
author_sort Abdurahman, Siti Waznah
title DNA barcoding of Arius species (Siluriformes: Ariidae) found in Peninsular Malaysia waters, using mitochondrial Cytochrome oxidase subunit I gene.
title_short DNA barcoding of Arius species (Siluriformes: Ariidae) found in Peninsular Malaysia waters, using mitochondrial Cytochrome oxidase subunit I gene.
title_full DNA barcoding of Arius species (Siluriformes: Ariidae) found in Peninsular Malaysia waters, using mitochondrial Cytochrome oxidase subunit I gene.
title_fullStr DNA barcoding of Arius species (Siluriformes: Ariidae) found in Peninsular Malaysia waters, using mitochondrial Cytochrome oxidase subunit I gene.
title_full_unstemmed DNA barcoding of Arius species (Siluriformes: Ariidae) found in Peninsular Malaysia waters, using mitochondrial Cytochrome oxidase subunit I gene.
title_sort dna barcoding of arius species (siluriformes: ariidae) found in peninsular malaysia waters, using mitochondrial cytochrome oxidase subunit i gene.
publishDate 2014
url http://irep.iium.edu.my/48139/
http://irep.iium.edu.my/48139/4/48139.pdf
first_indexed 2023-09-18T21:08:22Z
last_indexed 2023-09-18T21:08:22Z
_version_ 1777411085596360704

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