Ultraviolet/ozone treated polystyrene microcarriers for animal cell culture

BACKGROUND: Microcarrier based cell culture offers many advantages when compared with conventional cell cultures, and this technology has been widely used in the production of many important biological materials. In this work, microcarriers were produced using materials and methods that are relati...

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Main Authors:	Arifin, Mohd Azmir, Mel, Maizirwan, Samsudin, Nurhusna, Hashim, Yumi Zuhanis Has-Yun, Mohd. Salleh, Hamzah, Sopyan, Iis, Nordin, Norshariza
Format:	Article
Language:	English English
Published:	John WIley & Sons Ltd. 2015
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/48140/ http://irep.iium.edu.my/48140/ http://irep.iium.edu.my/48140/ http://irep.iium.edu.my/48140/1/10.1002-JCTB.4855_FINAL_2015.pdf http://irep.iium.edu.my/48140/4/48140%20Ultraviolet_ozone%20treated%20polystyrene%20SCOPUS.pdf

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spelling	iium-481402019-11-07T06:42:18Z http://irep.iium.edu.my/48140/ Ultraviolet/ozone treated polystyrene microcarriers for animal cell culture Arifin, Mohd Azmir Mel, Maizirwan Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Mohd. Salleh, Hamzah Sopyan, Iis Nordin, Norshariza TP248.13 Biotechnology BACKGROUND: Microcarrier based cell culture offers many advantages when compared with conventional cell cultures, and this technology has been widely used in the production of many important biological materials. In this work, microcarriers were produced using materials and methods that are relatively simple and low-cost. RESULTS: Polystyrene (PS) microspheres were prepared by using an emulsion solvent–evaporation method and the effects of process conditions on the size of microspheres were investigated. The mean size of microspheres decreased with increasing agitation speed, concentration of polyvinyl alcohol (PVA) and polymer to solvent ratios but increased with increasing oil to water phase ratios. To enhance its cell attachment abilities, surfaces of PSmicrosphereswere loadedwith oxygen functional groups by using an ultraviolet/ozone (UVO3) system. Analysis by Fourier transform infrared (FTIR), energy dispersive X-ray spectroscopy (EDX), toluidine blue O (TBO) assay and relative contact angle measurements showed increased surface oxygen concentration afte rUVO3 treatment. Results fromspinner flask culture revealed that UVO3 treated PS microspheres support the growth of Vero cells to high cell density. CONCLUSION: UVO3 treatment successfully rendered the hydrophobic surface of PS microspheres hydrophilic. This newly developed microcarrier could serve as a low-cost alternative to commercial microcarriers available in the market today. John WIley & Sons Ltd. 2015-11-23 Article PeerReviewed application/pdf en http://irep.iium.edu.my/48140/1/10.1002-JCTB.4855_FINAL_2015.pdf application/pdf en http://irep.iium.edu.my/48140/4/48140%20Ultraviolet_ozone%20treated%20polystyrene%20SCOPUS.pdf Arifin, Mohd Azmir and Mel, Maizirwan and Samsudin, Nurhusna and Hashim, Yumi Zuhanis Has-Yun and Mohd. Salleh, Hamzah and Sopyan, Iis and Nordin, Norshariza (2015) Ultraviolet/ozone treated polystyrene microcarriers for animal cell culture. Journal of Chemical Technology and Biotechnology. ISSN 0268-2575 E-ISSN 1097-4660 (In Press) http://onlinelibrary.wiley.com/doi/10.1002/jctb.4855/abstract DOI 10.1002/jctb.4855
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Arifin, Mohd Azmir Mel, Maizirwan Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Mohd. Salleh, Hamzah Sopyan, Iis Nordin, Norshariza Ultraviolet/ozone treated polystyrene microcarriers for animal cell culture
description	BACKGROUND: Microcarrier based cell culture offers many advantages when compared with conventional cell cultures, and this technology has been widely used in the production of many important biological materials. In this work, microcarriers were produced using materials and methods that are relatively simple and low-cost. RESULTS: Polystyrene (PS) microspheres were prepared by using an emulsion solvent–evaporation method and the effects of process conditions on the size of microspheres were investigated. The mean size of microspheres decreased with increasing agitation speed, concentration of polyvinyl alcohol (PVA) and polymer to solvent ratios but increased with increasing oil to water phase ratios. To enhance its cell attachment abilities, surfaces of PSmicrosphereswere loadedwith oxygen functional groups by using an ultraviolet/ozone (UVO3) system. Analysis by Fourier transform infrared (FTIR), energy dispersive X-ray spectroscopy (EDX), toluidine blue O (TBO) assay and relative contact angle measurements showed increased surface oxygen concentration afte rUVO3 treatment. Results fromspinner flask culture revealed that UVO3 treated PS microspheres support the growth of Vero cells to high cell density. CONCLUSION: UVO3 treatment successfully rendered the hydrophobic surface of PS microspheres hydrophilic. This newly developed microcarrier could serve as a low-cost alternative to commercial microcarriers available in the market today.
format	Article
author	Arifin, Mohd Azmir Mel, Maizirwan Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Mohd. Salleh, Hamzah Sopyan, Iis Nordin, Norshariza
author_facet	Arifin, Mohd Azmir Mel, Maizirwan Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Mohd. Salleh, Hamzah Sopyan, Iis Nordin, Norshariza
author_sort	Arifin, Mohd Azmir
title	Ultraviolet/ozone treated polystyrene microcarriers for animal cell culture
title_short	Ultraviolet/ozone treated polystyrene microcarriers for animal cell culture
title_full	Ultraviolet/ozone treated polystyrene microcarriers for animal cell culture
title_fullStr	Ultraviolet/ozone treated polystyrene microcarriers for animal cell culture
title_full_unstemmed	Ultraviolet/ozone treated polystyrene microcarriers for animal cell culture
title_sort	ultraviolet/ozone treated polystyrene microcarriers for animal cell culture
publisher	John WIley & Sons Ltd.
publishDate	2015
url	http://irep.iium.edu.my/48140/ http://irep.iium.edu.my/48140/ http://irep.iium.edu.my/48140/ http://irep.iium.edu.my/48140/1/10.1002-JCTB.4855_FINAL_2015.pdf http://irep.iium.edu.my/48140/4/48140%20Ultraviolet_ozone%20treated%20polystyrene%20SCOPUS.pdf
first_indexed	2023-09-18T21:08:22Z
last_indexed	2023-09-18T21:08:22Z
_version_	1777411085741064192

Ultraviolet/ozone treated polystyrene microcarriers for animal cell culture

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