Oxidative stress-mediated apoptosis induced by ethanolic mango seed extract in cultured estrogen receptor-positive breast cancer MCF-7 Cells

Oxidative stress-mediated apoptosis induced by ethanolic mango seed extract in cultured estrogen receptor-positive breast cancer MCF-7 Cells

Breast cancer has become a global health issue requiring huge expenditures for care and treatment of patients. There is a need to discover newer cost-effective alternatives for current therapeutic regimes. Mango kernel is a waste product with potential as a source of anti-cancer phytochemicals, esp...

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Main Authors: Al-Shwyeh, Hussah Abdullah, Abdulkarim , Sabo Mohammed, Rasedee, Abdullah, Mirghani, Mohamed Elwathig Saeed
Format: Article
Language:English
Published: MDPI 2015
Subjects:
Q Science (General)
Online Access:http://irep.iium.edu.my/48572/
http://irep.iium.edu.my/48572/
http://irep.iium.edu.my/48572/
http://irep.iium.edu.my/48572/1/ijms-16-03528_%28Hussah-_UPM%29.pdf
id iium-48572
recordtype eprints
spelling iium-485722016-05-09T08:50:43Z http://irep.iium.edu.my/48572/ Oxidative stress-mediated apoptosis induced by ethanolic mango seed extract in cultured estrogen receptor-positive breast cancer MCF-7 Cells Al-Shwyeh, Hussah Abdullah Abdulkarim , Sabo Mohammed Rasedee, Abdullah Mirghani, Mohamed Elwathig Saeed Q Science (General) Breast cancer has become a global health issue requiring huge expenditures for care and treatment of patients. There is a need to discover newer cost-effective alternatives for current therapeutic regimes. Mango kernel is a waste product with potential as a source of anti-cancer phytochemicals, especially since it is non-toxic towards normal breast cell lines at concentrations for which it induces cell death in breast cancer cells. In this study, the anti-cancer effect of mango kernel extract was determined on estrogen receptor-positive human breast carcinoma (MCF-7) cells. The MCF-7 cells were cultured and treated with 5, 10 and 50 μg/mL of mango kernel extract for 12 and 24 h. In response to treatment, there were time- and dose-dependent increases in oxidative stress markers and pro-apoptotic factors; Bcl-2-like protein 4 (BAX), p53, cytochrome c and caspases (7, 8 and 9) in the MCF-7 cells treated with the extract. At the same time, there were decreases in pro-survival markers (Bcl-2 and glutathione) as the result of the treatments. The changes induced in the MCF-7 cells by mango kernel extract treatment suggest that the extract can induce cancer cell apoptosis, likely via the activation of oxidative stress. These findings need to be evaluated further to determine whether mango kernel extract can be developed as an anti-breast cancer agent. MDPI 2015 Article PeerReviewed application/pdf en http://irep.iium.edu.my/48572/1/ijms-16-03528_%28Hussah-_UPM%29.pdf Al-Shwyeh, Hussah Abdullah and Abdulkarim , Sabo Mohammed and Rasedee, Abdullah and Mirghani, Mohamed Elwathig Saeed (2015) Oxidative stress-mediated apoptosis induced by ethanolic mango seed extract in cultured estrogen receptor-positive breast cancer MCF-7 Cells. International Journal of Molecular Sciences, 16 (2). pp. 3528-3536. ISSN 1661-6596 E-ISSN 1422-0067 Http://www.mdpi.com/journal/ijms doi:10.3390/ijms16023528
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic Q Science (General)
spellingShingle Q Science (General)
Al-Shwyeh, Hussah Abdullah
Abdulkarim , Sabo Mohammed
Rasedee, Abdullah
Mirghani, Mohamed Elwathig Saeed
Oxidative stress-mediated apoptosis induced by ethanolic mango seed extract in cultured estrogen receptor-positive breast cancer MCF-7 Cells
description Breast cancer has become a global health issue requiring huge expenditures for care and treatment of patients. There is a need to discover newer cost-effective alternatives for current therapeutic regimes. Mango kernel is a waste product with potential as a source of anti-cancer phytochemicals, especially since it is non-toxic towards normal breast cell lines at concentrations for which it induces cell death in breast cancer cells. In this study, the anti-cancer effect of mango kernel extract was determined on estrogen receptor-positive human breast carcinoma (MCF-7) cells. The MCF-7 cells were cultured and treated with 5, 10 and 50 μg/mL of mango kernel extract for 12 and 24 h. In response to treatment, there were time- and dose-dependent increases in oxidative stress markers and pro-apoptotic factors; Bcl-2-like protein 4 (BAX), p53, cytochrome c and caspases (7, 8 and 9) in the MCF-7 cells treated with the extract. At the same time, there were decreases in pro-survival markers (Bcl-2 and glutathione) as the result of the treatments. The changes induced in the MCF-7 cells by mango kernel extract treatment suggest that the extract can induce cancer cell apoptosis, likely via the activation of oxidative stress. These findings need to be evaluated further to determine whether mango kernel extract can be developed as an anti-breast cancer agent.
format Article
author Al-Shwyeh, Hussah Abdullah
Abdulkarim , Sabo Mohammed
Rasedee, Abdullah
Mirghani, Mohamed Elwathig Saeed
author_facet Al-Shwyeh, Hussah Abdullah
Abdulkarim , Sabo Mohammed
Rasedee, Abdullah
Mirghani, Mohamed Elwathig Saeed
author_sort Al-Shwyeh, Hussah Abdullah
title Oxidative stress-mediated apoptosis induced by ethanolic mango seed extract in cultured estrogen receptor-positive breast cancer MCF-7 Cells
title_short Oxidative stress-mediated apoptosis induced by ethanolic mango seed extract in cultured estrogen receptor-positive breast cancer MCF-7 Cells
title_full Oxidative stress-mediated apoptosis induced by ethanolic mango seed extract in cultured estrogen receptor-positive breast cancer MCF-7 Cells
title_fullStr Oxidative stress-mediated apoptosis induced by ethanolic mango seed extract in cultured estrogen receptor-positive breast cancer MCF-7 Cells
title_full_unstemmed Oxidative stress-mediated apoptosis induced by ethanolic mango seed extract in cultured estrogen receptor-positive breast cancer MCF-7 Cells
title_sort oxidative stress-mediated apoptosis induced by ethanolic mango seed extract in cultured estrogen receptor-positive breast cancer mcf-7 cells
publisher MDPI
publishDate 2015
url http://irep.iium.edu.my/48572/
http://irep.iium.edu.my/48572/
http://irep.iium.edu.my/48572/
http://irep.iium.edu.my/48572/1/ijms-16-03528_%28Hussah-_UPM%29.pdf
first_indexed 2023-09-18T21:08:52Z
last_indexed 2023-09-18T21:08:52Z
_version_ 1777411117771915264

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