Allicin-induced cellular destruction of haemoflagellate protozoa, Trypanosoma evansi in mice
Allicin or diallyl thiosulfinate (C3H5SS(O)C3H5), an active compound of garlic (Allium sativum) is known for its pharmaceutical properties. In this study, the cellular destructive effects of allicin on a haemoflagellate protozoa parasite Trypanosoma evansi was investigated. Groups of male ICR mice w...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Penerbit UKM
2011
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/48765/ http://irep.iium.edu.my/48765/ http://irep.iium.edu.my/48765/2/09%20B.A.H%20Zainal.pdf |
| Summary: | Allicin or diallyl thiosulfinate (C3H5SS(O)C3H5), an active compound of garlic (Allium sativum) is known for its pharmaceutical properties. In this study, the cellular destructive effects of allicin on a haemoflagellate protozoa parasite Trypanosoma evansi was investigated. Groups of male ICR mice were infected with a lethal dose of the parasite (1×105 parasites per mouse) and each group was either treated intraperitoneally with berenil (0.01 mL per mouse, a commercial anti-trypanosomal drug) on D+3 post-infection as the positive control group, treated orally with allicin (0.1 mL of 15 µg/mL allicin solution per mouse) for 30 days starting from D-7 pre-infection as the experimental group, or left untreated as the negative control group. Thin-stained blood smears were prepared from each mouse every alternate day, starting from D+3 post-infection and continued until the animal succumbed or until D+90 post-infection. Parasitaemias were determined using light microscope. Unstained blood smears were also prepared for direct observation under Phillips XL30 and Leo 1450VP scanning electron microscopes. All mice in the negative group succumbed to the infection with drastic increase of parasitaemias while all the positive control mice had minimal parasitaemias and cleared from the
infection and survived for more than 100 days. On the other hand mice in the experimental group, experienced a prolonged suppressed parasitaemias which became patent later and caused death to all mice. Micrograph observations of parasites in the positive group showed that the parasites had adverse morphological changes due to berenil treatment which lead to cell destruction and death within 5 – 6 hours post-treatment. Likewise parasites in the experimental group too had undergone profound physical damages which caused cell death. This is the first report which shows that allicin actually induced cellular damage to haemoflagellate cells of T. evansi in vivo.
Alisin atau ‘diallyl thiosulfinate’ (C3
H5
SS(O)C3
H5
) adalah sebatian aktif bawang putih (Allium sativum) yang mempunyai
banyak khasiat farmaseutikal. Dalam kajian ini kesan kemusnahan alisin ke atas sel parasit protozoa hemoflagelat
Trypanosoma evansi telah diselidiki. Kumpulan-kumpulan mencit jantan strain ICR telah dijangkitkan dengan takaran
maut parasit (1×105
parasit per mencit) dan setiap kumpulan mencit sama ada dirawat dengan berenil iaitu sejenis
dadah anti-tripanosom kemersial secara suntikan intraperitonem pada hari D+3 pasca-jangkitan (kumpulan kawalan
positif), atau mencit dirawat dengan alisin secara oral (0.1 mL of 15 µg/mL larutan alisin per mencit) selama 30 hari
bermula pada hari D-7 pasca-jangkitan (kumpulan kajian), atau mencit tidak mendapat sebarang rawatan (kumpulan
kawalan negatif). Slaid darah calitan nipis terwarna disediakan daripada setiap ekor mencit berselang hari bermula
pada hari D+3 sehingga mencit maut atau sehingga hari D+90 pos-jangkitan. Parasitemia telah ditentukan di bawah
mikroskop cahaya. Calitan darah tak berwarna turut disediakan untuk cerapan langsung menggunakan mikroskop
elektron pengimbasan Phillips XL30 dan Leo 1450VP. Didapati semua mencit kumpulan kawalan negatif mati akibat
peningktan parasitemia yang cepat manakala mencit dalam kumpulan kawalan positif menunjukkan parasitemia yang
minima dan bebas daripada jangkitan parasit dan bermandiri melebihi 100 hari. Sebaliknya mencit dalam kumpulan
kajian mengalami perkembangan parasitemia tertindas yang kemudiannya meningkat semula dan menyebabkan kematian
mencit. Cerapan ke atas mikrograf darah daripada kumpulan kawalan positif menunjukkan morfologi parasit mengalami
kerosakan teruk kesan tindakan berenil yang menyebabkan kemusnahan dan kematian sel parasit 5-6 jam pasca-rawatan.
Sebagai perbandingan parasit daripada kumpulan kajian juga mengalami kerosakan fizikal yang parah dan membawa
kepada kematian. Hasil kajian ini merupakan laporan pertama yang menunjukkan alisin mengaruh kemusnahan sel-sel
parasit T. evansi secara in vivo.
|
|---|