In-vivo antimalarial activity of the endophytic actinobacterium, Streptomyces SUK 10

Endophytic bacteria, such as Streptomyces, have the potential to act as a source for novel bioactive molecules with medicinal properties. The present study was aimed at assessing the antimalarial activity of crude extract isolated from various strains of actinobacteria living endophytically in some...

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Main Authors:	Baba, Mohd Shukri, Mohamad Zin, Noraziah, Abu Hassan, Zainal Abidin, Latip, Jalifah, Pethick, Florence, Hunter, Iain S., Edrada-Ebel, RuAngelie, Herron, Paul R.
Format:	Article
Language:	English English
Published:	The Microbiological Society of Korea 2015
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/48801/ http://irep.iium.edu.my/48801/ http://irep.iium.edu.my/48801/1/Journal_No._5.pdf http://irep.iium.edu.my/48801/2/10.1007%252Fs12275-015-5076-6

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spelling	iium-488012016-05-31T06:51:01Z http://irep.iium.edu.my/48801/ In-vivo antimalarial activity of the endophytic actinobacterium, Streptomyces SUK 10 Baba, Mohd Shukri Mohamad Zin, Noraziah Abu Hassan, Zainal Abidin Latip, Jalifah Pethick, Florence Hunter, Iain S. Edrada-Ebel, RuAngelie Herron, Paul R. QR Microbiology Endophytic bacteria, such as Streptomyces, have the potential to act as a source for novel bioactive molecules with medicinal properties. The present study was aimed at assessing the antimalarial activity of crude extract isolated from various strains of actinobacteria living endophytically in some Malaysian medicinal plants. Using the four day suppression test method on male ICR strain mice, compounds produced from three strains of Streptomyces (SUK8, SUK10, and SUK27) were tested in vivo against Plasmodium berghei PZZ1/100 in an antimalarial screen using crude extracts at four different concentrations. One of these extracts, isolated from Streptomyces SUK10 obtained from the bark of Shorea ovalis tree, showed inhibition of the test organism and was further tested against P. berghei-infected mice for antimalarial activity at different concentrations. There was a positive relationship between the survival of the infected mouse group treated with 50 μg/kg body weight (bw) of ethyl acetate-SUK10 crude extract and the ability to inhibit the parasites growth. The parasite inhibition percentage for this group showed that 50% of the mice survived for more than 90 days after infection with the parasite. The nucleotide sequence and phylogenetic tree suggested that Streptomyces SUK10 may constitute a new species within the Streptomyces genus. As part of the drug discovery process, these promising finding may contribute to the medicinal and pharmaceutical field for malarial treatment. The Microbiological Society of Korea 2015-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/48801/1/Journal_No._5.pdf application/pdf en http://irep.iium.edu.my/48801/2/10.1007%252Fs12275-015-5076-6 Baba, Mohd Shukri and Mohamad Zin, Noraziah and Abu Hassan, Zainal Abidin and Latip, Jalifah and Pethick, Florence and Hunter, Iain S. and Edrada-Ebel, RuAngelie and Herron, Paul R. (2015) In-vivo antimalarial activity of the endophytic actinobacterium, Streptomyces SUK 10. Journal of Microbiology, 53 (12). pp. 847-855. ISSN 1225-8873 E-ISSN 1976-3794 DOI 10.1007/s12275-015-5076-6
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QR Microbiology
spellingShingle	QR Microbiology Baba, Mohd Shukri Mohamad Zin, Noraziah Abu Hassan, Zainal Abidin Latip, Jalifah Pethick, Florence Hunter, Iain S. Edrada-Ebel, RuAngelie Herron, Paul R. In-vivo antimalarial activity of the endophytic actinobacterium, Streptomyces SUK 10
description	Endophytic bacteria, such as Streptomyces, have the potential to act as a source for novel bioactive molecules with medicinal properties. The present study was aimed at assessing the antimalarial activity of crude extract isolated from various strains of actinobacteria living endophytically in some Malaysian medicinal plants. Using the four day suppression test method on male ICR strain mice, compounds produced from three strains of Streptomyces (SUK8, SUK10, and SUK27) were tested in vivo against Plasmodium berghei PZZ1/100 in an antimalarial screen using crude extracts at four different concentrations. One of these extracts, isolated from Streptomyces SUK10 obtained from the bark of Shorea ovalis tree, showed inhibition of the test organism and was further tested against P. berghei-infected mice for antimalarial activity at different concentrations. There was a positive relationship between the survival of the infected mouse group treated with 50 μg/kg body weight (bw) of ethyl acetate-SUK10 crude extract and the ability to inhibit the parasites growth. The parasite inhibition percentage for this group showed that 50% of the mice survived for more than 90 days after infection with the parasite. The nucleotide sequence and phylogenetic tree suggested that Streptomyces SUK10 may constitute a new species within the Streptomyces genus. As part of the drug discovery process, these promising finding may contribute to the medicinal and pharmaceutical field for malarial treatment.
format	Article
author	Baba, Mohd Shukri Mohamad Zin, Noraziah Abu Hassan, Zainal Abidin Latip, Jalifah Pethick, Florence Hunter, Iain S. Edrada-Ebel, RuAngelie Herron, Paul R.
author_facet	Baba, Mohd Shukri Mohamad Zin, Noraziah Abu Hassan, Zainal Abidin Latip, Jalifah Pethick, Florence Hunter, Iain S. Edrada-Ebel, RuAngelie Herron, Paul R.
author_sort	Baba, Mohd Shukri
title	In-vivo antimalarial activity of the endophytic actinobacterium, Streptomyces SUK 10
title_short	In-vivo antimalarial activity of the endophytic actinobacterium, Streptomyces SUK 10
title_full	In-vivo antimalarial activity of the endophytic actinobacterium, Streptomyces SUK 10
title_fullStr	In-vivo antimalarial activity of the endophytic actinobacterium, Streptomyces SUK 10
title_full_unstemmed	In-vivo antimalarial activity of the endophytic actinobacterium, Streptomyces SUK 10
title_sort	in-vivo antimalarial activity of the endophytic actinobacterium, streptomyces suk 10
publisher	The Microbiological Society of Korea
publishDate	2015
url	http://irep.iium.edu.my/48801/ http://irep.iium.edu.my/48801/ http://irep.iium.edu.my/48801/1/Journal_No._5.pdf http://irep.iium.edu.my/48801/2/10.1007%252Fs12275-015-5076-6
first_indexed	2023-09-18T21:09:09Z
last_indexed	2023-09-18T21:09:09Z
_version_	1777411135299911680

In-vivo antimalarial activity of the endophytic actinobacterium, Streptomyces SUK 10

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