Determination and quantification of ethanol and byproducts using HPLC (Brand: WATERS)

Ethanol has extensive applications especially as a solvent and a feedstock for synthesis of other products. It also has other uses such as for consumption (including scents and flavourings), colourings, medicines, personal care products, cleaning products, and can be served as solvent in perfume, ae...

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Main Author:	Azmi, Azlin Suhaida
Other Authors:	Jamal, Parveen
Format:	Book Chapter
Language:	English English
Published:	IIUM Press 2016
Subjects:	TP155 Chemical engineering
Online Access:	http://irep.iium.edu.my/48901/ http://irep.iium.edu.my/48901/ http://irep.iium.edu.my/48901/2/Ch_6_-_Dr_Azlin_Determination_of_carbohydrates_and_its_derives_using_fast_HPLC_analysis_reviewed_edited-Murni.pdf http://irep.iium.edu.my/48901/5/experimental__methods_modern_biotechnology.pdf

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recordtype	eprints
spelling	iium-489012016-08-25T08:17:59Z http://irep.iium.edu.my/48901/ Determination and quantification of ethanol and byproducts using HPLC (Brand: WATERS) Azmi, Azlin Suhaida TP155 Chemical engineering Ethanol has extensive applications especially as a solvent and a feedstock for synthesis of other products. It also has other uses such as for consumption (including scents and flavourings), colourings, medicines, personal care products, cleaning products, and can be served as solvent in perfume, aerosols, paint, lacquer and explosive (O'Leary, 2000). On top of that, ethanol is also used as an alternative fuel which is usually blended with gasoline and is used in unmodified car engine. Non-petroleum-based ethanol is produced from biological sources such as sugar, starch or cellulose. Depend on which type of the sources, the conversion steps varies and produce byproducts. For an example, the conversion of polysaccharide starch into ethanol which is an indirect fermentation normally takes three separate steps. The first step is liquefaction using -amylase enzyme, which reduces the viscosity of the starch and fragments the starch into regularly sized chains, to yield dextrin, maltose, maltotriose and maltopentose followed by saccharification, whereby the starch is converted into sugar using glucoamylase enzyme. The final step involves the fermentation of sugar into ethanol using yeast. Depend on type of microorganisms; the fermentation will yield several other byproducts such as glycerol, lactic acid and acetic acid. The soluble sugar can be detected and quantified using HPLC with refractive index detection (Sluiter, Hames, Ruiz, Scarlata, Sluiter, & Templeton, 2008). Column such as IC-Pak Ion Exclusion with pre-column (SH-1011P) connected to HPLC system can capture most of these byproducts together with ethanol in a short time. IIUM Press Jamal, Parveen Noorbatcha, Ibrahim Ali Azmi, Azlin Suhaida 2016 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/48901/2/Ch_6_-_Dr_Azlin_Determination_of_carbohydrates_and_its_derives_using_fast_HPLC_analysis_reviewed_edited-Murni.pdf application/pdf en http://irep.iium.edu.my/48901/5/experimental__methods_modern_biotechnology.pdf Azmi, Azlin Suhaida (2016) Determination and quantification of ethanol and byproducts using HPLC (Brand: WATERS). In: Experimental Methods in Modern Biotechnology. IIUM Press, Malaysia, pp. 47-52. ISBN 978-967-418-384-4 http://iiumpress.iium.edu.my/bookshop/experimental-methods-in-modern-biotechnology
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP155 Chemical engineering
spellingShingle	TP155 Chemical engineering Azmi, Azlin Suhaida Determination and quantification of ethanol and byproducts using HPLC (Brand: WATERS)
description	Ethanol has extensive applications especially as a solvent and a feedstock for synthesis of other products. It also has other uses such as for consumption (including scents and flavourings), colourings, medicines, personal care products, cleaning products, and can be served as solvent in perfume, aerosols, paint, lacquer and explosive (O'Leary, 2000). On top of that, ethanol is also used as an alternative fuel which is usually blended with gasoline and is used in unmodified car engine. Non-petroleum-based ethanol is produced from biological sources such as sugar, starch or cellulose. Depend on which type of the sources, the conversion steps varies and produce byproducts. For an example, the conversion of polysaccharide starch into ethanol which is an indirect fermentation normally takes three separate steps. The first step is liquefaction using -amylase enzyme, which reduces the viscosity of the starch and fragments the starch into regularly sized chains, to yield dextrin, maltose, maltotriose and maltopentose followed by saccharification, whereby the starch is converted into sugar using glucoamylase enzyme. The final step involves the fermentation of sugar into ethanol using yeast. Depend on type of microorganisms; the fermentation will yield several other byproducts such as glycerol, lactic acid and acetic acid. The soluble sugar can be detected and quantified using HPLC with refractive index detection (Sluiter, Hames, Ruiz, Scarlata, Sluiter, & Templeton, 2008). Column such as IC-Pak Ion Exclusion with pre-column (SH-1011P) connected to HPLC system can capture most of these byproducts together with ethanol in a short time.
author2	Jamal, Parveen
author_facet	Jamal, Parveen Azmi, Azlin Suhaida
format	Book Chapter
author	Azmi, Azlin Suhaida
author_sort	Azmi, Azlin Suhaida
title	Determination and quantification of ethanol and byproducts using HPLC (Brand: WATERS)
title_short	Determination and quantification of ethanol and byproducts using HPLC (Brand: WATERS)
title_full	Determination and quantification of ethanol and byproducts using HPLC (Brand: WATERS)
title_fullStr	Determination and quantification of ethanol and byproducts using HPLC (Brand: WATERS)
title_full_unstemmed	Determination and quantification of ethanol and byproducts using HPLC (Brand: WATERS)
title_sort	determination and quantification of ethanol and byproducts using hplc (brand: waters)
publisher	IIUM Press
publishDate	2016
url	http://irep.iium.edu.my/48901/ http://irep.iium.edu.my/48901/ http://irep.iium.edu.my/48901/2/Ch_6_-_Dr_Azlin_Determination_of_carbohydrates_and_its_derives_using_fast_HPLC_analysis_reviewed_edited-Murni.pdf http://irep.iium.edu.my/48901/5/experimental__methods_modern_biotechnology.pdf
first_indexed	2023-09-18T21:09:15Z
last_indexed	2023-09-18T21:09:15Z
_version_	1777411141773819904

Determination and quantification of ethanol and byproducts using HPLC (Brand: WATERS)

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