Isolation of bacteria from acidic peat swamp forest soil and their lignin degradation potential

The tropical peat swamp forest in Malaysia has reduced significantly due to increasing pressure for development and demand for agricultural land. Pekan peat swamp forest is part of the 200,000 hectares of peat swamp forest located in Pahang, Peninsular Malaysia. While more extensive study were done...

Full description

Bibliographic Details
Main Authors:	Mohamad Roslan, Muhamad Aidilfitri, Amirudin, Nur Azam, Zainal Abidin, Zaima Azira, Mohd Omar, Suhaila
Format:	Conference or Workshop Item
Language:	English English
Published:	2015
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/49226/ http://irep.iium.edu.my/49226/1/poster_icast2015.pdf http://irep.iium.edu.my/49226/4/iCAST2015_aidil.pdf

id	iium-49226
recordtype	eprints
spelling	iium-492262017-10-05T03:29:00Z http://irep.iium.edu.my/49226/ Isolation of bacteria from acidic peat swamp forest soil and their lignin degradation potential Mohamad Roslan, Muhamad Aidilfitri Amirudin, Nur Azam Zainal Abidin, Zaima Azira Mohd Omar, Suhaila Q Science (General) The tropical peat swamp forest in Malaysia has reduced significantly due to increasing pressure for development and demand for agricultural land. Pekan peat swamp forest is part of the 200,000 hectares of peat swamp forest located in Pahang, Peninsular Malaysia. While more extensive study were done on flora and fauna, the study on microbial diversity in this habitat is very limited. The highly acidic environment, low concentrations of nutrients and anoxic condition of the peat are among challenges that hampered the cultivation of microorganism from this environment. In this study two types of agar-based medium, M1 minimal medium and peat agar supplemented with glucose, methanol and lignin were used to isolate bacteria from the peat sediment. In comparison to M1 minimal medium, the use of peat agar has resulted with higher number of isolates with different morphologies. The peat agar mainly contains the acidic peat water that was collected from the sampling location. Based on the growth on medium supplemented with lignin selected isolates were identified using 16s rDNA sequencing. At least three of the isolates showed sequence similarity to Burkholderia species which is one of the common species studied on their ligninase-producing abilities. The results from this study serve as the preliminary data for further work on growth characteristics and enzymatic potential of isolates from acidic peat swamp sediment. 2015-08-10 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/49226/1/poster_icast2015.pdf application/pdf en http://irep.iium.edu.my/49226/4/iCAST2015_aidil.pdf Mohamad Roslan, Muhamad Aidilfitri and Amirudin, Nur Azam and Zainal Abidin, Zaima Azira and Mohd Omar, Suhaila (2015) Isolation of bacteria from acidic peat swamp forest soil and their lignin degradation potential. In: International Conference on Advancement in Science and Technology (iCAST 2015) - ‘Frontiers in Biotechnology’, 10th-12th August 2015, Cherating, Pahang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Mohamad Roslan, Muhamad Aidilfitri Amirudin, Nur Azam Zainal Abidin, Zaima Azira Mohd Omar, Suhaila Isolation of bacteria from acidic peat swamp forest soil and their lignin degradation potential
description	The tropical peat swamp forest in Malaysia has reduced significantly due to increasing pressure for development and demand for agricultural land. Pekan peat swamp forest is part of the 200,000 hectares of peat swamp forest located in Pahang, Peninsular Malaysia. While more extensive study were done on flora and fauna, the study on microbial diversity in this habitat is very limited. The highly acidic environment, low concentrations of nutrients and anoxic condition of the peat are among challenges that hampered the cultivation of microorganism from this environment. In this study two types of agar-based medium, M1 minimal medium and peat agar supplemented with glucose, methanol and lignin were used to isolate bacteria from the peat sediment. In comparison to M1 minimal medium, the use of peat agar has resulted with higher number of isolates with different morphologies. The peat agar mainly contains the acidic peat water that was collected from the sampling location. Based on the growth on medium supplemented with lignin selected isolates were identified using 16s rDNA sequencing. At least three of the isolates showed sequence similarity to Burkholderia species which is one of the common species studied on their ligninase-producing abilities. The results from this study serve as the preliminary data for further work on growth characteristics and enzymatic potential of isolates from acidic peat swamp sediment.
format	Conference or Workshop Item
author	Mohamad Roslan, Muhamad Aidilfitri Amirudin, Nur Azam Zainal Abidin, Zaima Azira Mohd Omar, Suhaila
author_facet	Mohamad Roslan, Muhamad Aidilfitri Amirudin, Nur Azam Zainal Abidin, Zaima Azira Mohd Omar, Suhaila
author_sort	Mohamad Roslan, Muhamad Aidilfitri
title	Isolation of bacteria from acidic peat swamp forest soil and their lignin degradation potential
title_short	Isolation of bacteria from acidic peat swamp forest soil and their lignin degradation potential
title_full	Isolation of bacteria from acidic peat swamp forest soil and their lignin degradation potential
title_fullStr	Isolation of bacteria from acidic peat swamp forest soil and their lignin degradation potential
title_full_unstemmed	Isolation of bacteria from acidic peat swamp forest soil and their lignin degradation potential
title_sort	isolation of bacteria from acidic peat swamp forest soil and their lignin degradation potential
publishDate	2015
url	http://irep.iium.edu.my/49226/ http://irep.iium.edu.my/49226/1/poster_icast2015.pdf http://irep.iium.edu.my/49226/4/iCAST2015_aidil.pdf
first_indexed	2023-09-18T21:09:37Z
last_indexed	2023-09-18T21:09:37Z
_version_	1777411164566716416

Isolation of bacteria from acidic peat swamp forest soil and their lignin degradation potential

Similar Items