Screening for ligninase-producing bacteria from South East Pahang peat swamp forest soil

Research on lignin degradation capability is previously restricted exclusively to fungi’s enzymes. However, recent studies revealed ranges of soil bacteria that able to produce ligninolytic enzymes. In fact, bacterial enzyme has some advantages over fungal enzymes such as in aspects of thermostabi...

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Main Authors:	Mohamad Roslan, Muhamad Aidilfitri, Zainal Abidin, Zaima Azira, Mohd Omar, Suhaila
Format:	Conference or Workshop Item
Language:	English
Published:	Malaysian Society for Microbiology 2015
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/49227/ http://irep.iium.edu.my/49227/ http://irep.iium.edu.my/49227/8/49227_Screening%20for%20ligninase-producing_complete.pdf

id	iium-49227
recordtype	eprints
spelling	iium-492272017-10-05T03:09:27Z http://irep.iium.edu.my/49227/ Screening for ligninase-producing bacteria from South East Pahang peat swamp forest soil Mohamad Roslan, Muhamad Aidilfitri Zainal Abidin, Zaima Azira Mohd Omar, Suhaila Q Science (General) Research on lignin degradation capability is previously restricted exclusively to fungi’s enzymes. However, recent studies revealed ranges of soil bacteria that able to produce ligninolytic enzymes. In fact, bacterial enzyme has some advantages over fungal enzymes such as in aspects of thermostability, acidic stability and mediator dependency. The present study concentrates on isolation and screening of ligninase-producing bacteria from South East Pahang Peat Swamp Forest (SEPPSF) soil using agar-based assay. Thirteen morphologically distinctive isolates were selected and able to show decolourization zone on Azure B plates. The ratio of decolourization zones were measured to the ratio of the colony size. Only four of the thirteen isolates were able to grow on lignin plates. Subsequently, they were tested on M1 agar supplemented with ligninolytic enzyme indicator compounds which were tannic acid (TA), guaiacol and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS). All four isolates showed growth on TA plates. Intense brown colour formation was observed around the colony of isolates AR3 and AR10 on guaiacol plates while none exhibited green coloration around the colonies when tested on ABTS plates. 16S rRNA gene sequencing and analysis indicated AR3, AR8 and AR13 as Burkholderia sp. while AR10 as Serratia sp. Based on the agar-based screening results, AR10 was perceived as potential ligninase-producing bacteria. More experiments would be conducted in the future to characterize AR10 and the ligninolytic enzymes produced. Malaysian Society for Microbiology 2015-12 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/49227/8/49227_Screening%20for%20ligninase-producing_complete.pdf Mohamad Roslan, Muhamad Aidilfitri and Zainal Abidin, Zaima Azira and Mohd Omar, Suhaila (2015) Screening for ligninase-producing bacteria from South East Pahang peat swamp forest soil. In: International Congress of the Malaysian Society of Microbiology (Microbiology Meeting the needs of a changing world held 7-10 December 2015,, 7-10 Dec 2015, Bayview Beach Resort, Penang, Malaysia. (Unpublished) http://www.mymicro.org/ICMSM%202015%20Brochure.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Mohamad Roslan, Muhamad Aidilfitri Zainal Abidin, Zaima Azira Mohd Omar, Suhaila Screening for ligninase-producing bacteria from South East Pahang peat swamp forest soil
description	Research on lignin degradation capability is previously restricted exclusively to fungi’s enzymes. However, recent studies revealed ranges of soil bacteria that able to produce ligninolytic enzymes. In fact, bacterial enzyme has some advantages over fungal enzymes such as in aspects of thermostability, acidic stability and mediator dependency. The present study concentrates on isolation and screening of ligninase-producing bacteria from South East Pahang Peat Swamp Forest (SEPPSF) soil using agar-based assay. Thirteen morphologically distinctive isolates were selected and able to show decolourization zone on Azure B plates. The ratio of decolourization zones were measured to the ratio of the colony size. Only four of the thirteen isolates were able to grow on lignin plates. Subsequently, they were tested on M1 agar supplemented with ligninolytic enzyme indicator compounds which were tannic acid (TA), guaiacol and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS). All four isolates showed growth on TA plates. Intense brown colour formation was observed around the colony of isolates AR3 and AR10 on guaiacol plates while none exhibited green coloration around the colonies when tested on ABTS plates. 16S rRNA gene sequencing and analysis indicated AR3, AR8 and AR13 as Burkholderia sp. while AR10 as Serratia sp. Based on the agar-based screening results, AR10 was perceived as potential ligninase-producing bacteria. More experiments would be conducted in the future to characterize AR10 and the ligninolytic enzymes produced.
format	Conference or Workshop Item
author	Mohamad Roslan, Muhamad Aidilfitri Zainal Abidin, Zaima Azira Mohd Omar, Suhaila
author_facet	Mohamad Roslan, Muhamad Aidilfitri Zainal Abidin, Zaima Azira Mohd Omar, Suhaila
author_sort	Mohamad Roslan, Muhamad Aidilfitri
title	Screening for ligninase-producing bacteria from South East Pahang peat swamp forest soil
title_short	Screening for ligninase-producing bacteria from South East Pahang peat swamp forest soil
title_full	Screening for ligninase-producing bacteria from South East Pahang peat swamp forest soil
title_fullStr	Screening for ligninase-producing bacteria from South East Pahang peat swamp forest soil
title_full_unstemmed	Screening for ligninase-producing bacteria from South East Pahang peat swamp forest soil
title_sort	screening for ligninase-producing bacteria from south east pahang peat swamp forest soil
publisher	Malaysian Society for Microbiology
publishDate	2015
url	http://irep.iium.edu.my/49227/ http://irep.iium.edu.my/49227/ http://irep.iium.edu.my/49227/8/49227_Screening%20for%20ligninase-producing_complete.pdf
first_indexed	2023-09-18T21:09:37Z
last_indexed	2023-09-18T21:09:37Z
_version_	1777411164712468480

Screening for ligninase-producing bacteria from South East Pahang peat swamp forest soil

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