The chemopreventive effects of Nigella sativa extract and Thymoquinone in a paternal toxicity study

Studies have reported that exposure to cyclophosphamide (CPA) for cancer treatment result in adverse reproductive consequences. Over-production of reactive oxygen species causes oxidative stress thus affecting the DNA in male germ cell thus induces sperm defects. Our goal is to assess the potential...

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Main Authors:	Abdul Samat, Nadia Hanis, Abdul Rahman, Suzanah, Ahmad, Nur Amalina
Format:	Conference or Workshop Item
Language:	English English
Published:	2015
Subjects:	RM Therapeutics. Pharmacology RM300 Drugs and their action
Online Access:	http://irep.iium.edu.my/49400/ http://irep.iium.edu.my/49400/10/49400-program%20book.pdf http://irep.iium.edu.my/49400/1/iCAST_Nadia_Suzanah_KAHS_PDF.pdf

id	iium-49400
recordtype	eprints
spelling	iium-494002017-05-08T02:29:30Z http://irep.iium.edu.my/49400/ The chemopreventive effects of Nigella sativa extract and Thymoquinone in a paternal toxicity study Abdul Samat, Nadia Hanis Abdul Rahman, Suzanah Ahmad, Nur Amalina RM Therapeutics. Pharmacology RM300 Drugs and their action Studies have reported that exposure to cyclophosphamide (CPA) for cancer treatment result in adverse reproductive consequences. Over-production of reactive oxygen species causes oxidative stress thus affecting the DNA in male germ cell thus induces sperm defects. Our goal is to assess the potential effect of Nigella sativa extract (NSE) and thymoquinone (TQ) on sperm and embryo quality following fertlization from sperm produced from germ cells which have been exposed to the damaging alkylating effects of CPA. Thirty Balb/c mice were divided into six groups; (i) Control, (ii) NSE only, (iii) TQ only, (iv) CPA only, (v) CPA with NSE and (vi) CPA with TQ. Treatment was given to the mice based on the grouping for 32 days where the dosage for CPA is 200mg/kg and for NSE and TQ 10mg/kg. Groups (v) and (vi) were treated with CPA followed by NSE and TQ after six hours. Animals were sacrificed at 33rd days by cervical dislocation and sperm from testis and epidydymis were taken for in vitro fertilization (IVF) with eggs from untreated female and sperm analysis. Fertilization rates and embryo development were assessed and monitored for 5 days. TQ and NSE supplementation to CPA-exposed male mice have no significant effect (p>0.05) on the total number of sperm when compared to non treated CPA-exposed mice (p value = 0.498). NSE supplementation has been shown to have significant effect (p<0.05) on the percentage of double stranded DNA in the sperm head (p value = 0.034). The result shows an increment in fertilization rate in IVF if the CPA exposure is followed by the treatment with NSE (p value = 0.014, p<0.05). Finally, the embryo quality based on embryo scoring by Baczkowski (2004), was not affected by the supplementation of TQ and NSE to CPA-exposed male mice (p value = 0.235). Overall, both NSE and TQ have the chemoprotective potential against the cytotoxicity of cyclophosphamide on the reproductive capacity and fertility in the animal model. 2015 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/49400/10/49400-program%20book.pdf application/pdf en http://irep.iium.edu.my/49400/1/iCAST_Nadia_Suzanah_KAHS_PDF.pdf Abdul Samat, Nadia Hanis and Abdul Rahman, Suzanah and Ahmad, Nur Amalina (2015) The chemopreventive effects of Nigella sativa extract and Thymoquinone in a paternal toxicity study. In: International Conference on Advancement in Science and Technology (iCAST 2015) - ‘Frontiers in Biotechnology’, 10th-12th August 2015, Cherating, Pahang, Malaysia. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RM Therapeutics. Pharmacology RM300 Drugs and their action
spellingShingle	RM Therapeutics. Pharmacology RM300 Drugs and their action Abdul Samat, Nadia Hanis Abdul Rahman, Suzanah Ahmad, Nur Amalina The chemopreventive effects of Nigella sativa extract and Thymoquinone in a paternal toxicity study
description	Studies have reported that exposure to cyclophosphamide (CPA) for cancer treatment result in adverse reproductive consequences. Over-production of reactive oxygen species causes oxidative stress thus affecting the DNA in male germ cell thus induces sperm defects. Our goal is to assess the potential effect of Nigella sativa extract (NSE) and thymoquinone (TQ) on sperm and embryo quality following fertlization from sperm produced from germ cells which have been exposed to the damaging alkylating effects of CPA. Thirty Balb/c mice were divided into six groups; (i) Control, (ii) NSE only, (iii) TQ only, (iv) CPA only, (v) CPA with NSE and (vi) CPA with TQ. Treatment was given to the mice based on the grouping for 32 days where the dosage for CPA is 200mg/kg and for NSE and TQ 10mg/kg. Groups (v) and (vi) were treated with CPA followed by NSE and TQ after six hours. Animals were sacrificed at 33rd days by cervical dislocation and sperm from testis and epidydymis were taken for in vitro fertilization (IVF) with eggs from untreated female and sperm analysis. Fertilization rates and embryo development were assessed and monitored for 5 days. TQ and NSE supplementation to CPA-exposed male mice have no significant effect (p>0.05) on the total number of sperm when compared to non treated CPA-exposed mice (p value = 0.498). NSE supplementation has been shown to have significant effect (p<0.05) on the percentage of double stranded DNA in the sperm head (p value = 0.034). The result shows an increment in fertilization rate in IVF if the CPA exposure is followed by the treatment with NSE (p value = 0.014, p<0.05). Finally, the embryo quality based on embryo scoring by Baczkowski (2004), was not affected by the supplementation of TQ and NSE to CPA-exposed male mice (p value = 0.235). Overall, both NSE and TQ have the chemoprotective potential against the cytotoxicity of cyclophosphamide on the reproductive capacity and fertility in the animal model.
format	Conference or Workshop Item
author	Abdul Samat, Nadia Hanis Abdul Rahman, Suzanah Ahmad, Nur Amalina
author_facet	Abdul Samat, Nadia Hanis Abdul Rahman, Suzanah Ahmad, Nur Amalina
author_sort	Abdul Samat, Nadia Hanis
title	The chemopreventive effects of Nigella sativa extract and Thymoquinone in a paternal toxicity study
title_short	The chemopreventive effects of Nigella sativa extract and Thymoquinone in a paternal toxicity study
title_full	The chemopreventive effects of Nigella sativa extract and Thymoquinone in a paternal toxicity study
title_fullStr	The chemopreventive effects of Nigella sativa extract and Thymoquinone in a paternal toxicity study
title_full_unstemmed	The chemopreventive effects of Nigella sativa extract and Thymoquinone in a paternal toxicity study
title_sort	chemopreventive effects of nigella sativa extract and thymoquinone in a paternal toxicity study
publishDate	2015
url	http://irep.iium.edu.my/49400/ http://irep.iium.edu.my/49400/10/49400-program%20book.pdf http://irep.iium.edu.my/49400/1/iCAST_Nadia_Suzanah_KAHS_PDF.pdf
first_indexed	2023-09-18T21:09:51Z
last_indexed	2023-09-18T21:09:51Z
_version_	1777411179828740096

The chemopreventive effects of Nigella sativa extract and Thymoquinone in a paternal toxicity study

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