Infrared–metabolomics approach in detecting changes in andrographis paniculata metabolites due to different harvesting ages and times

BACKGROUND: The metabolite changes in three germplasm accessions of Malaysia Andrographis paniculata (Burm. F.) Nees, viz. 11265 (H), 11341 (P) and 11248 (T), due to their different harvesting ages and times were successfully evaluated by attenuated total reflectance (ATR)–Fourier transform infrared...

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Main Authors:	Yusof, Nur A'thifah, Isha, Azizul, Ismail, Intan Safinar, Khatib, Alfi, Shaari, Khozirah, Abas, Faridah, Rukayadi, Yaya
Format:	Article
Language:	English
Published:	John Wiley & Sons 2015
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/49725/ http://irep.iium.edu.my/49725/ http://irep.iium.edu.my/49725/ http://irep.iium.edu.my/49725/1/Yusof_et_al_2015_%281%29.pdf

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recordtype	eprints
spelling	iium-497252017-11-21T08:01:33Z http://irep.iium.edu.my/49725/ Infrared–metabolomics approach in detecting changes in andrographis paniculata metabolites due to different harvesting ages and times Yusof, Nur A'thifah Isha, Azizul Ismail, Intan Safinar Khatib, Alfi Shaari, Khozirah Abas, Faridah Rukayadi, Yaya QD Chemistry BACKGROUND: The metabolite changes in three germplasm accessions of Malaysia Andrographis paniculata (Burm. F.) Nees, viz. 11265 (H), 11341 (P) and 11248 (T), due to their different harvesting ages and times were successfully evaluated by attenuated total reflectance (ATR)–Fourier transform infrared (FTIR) spectroscopy and translated through multivariate data analysis of principal component analysis (PCA) and orthogonal partial least square–discriminant analysis (OPLS-DA). This present study revealed the feasibility of ATR-FTIR in detecting the trend changes of the major metabolites – andrographolide and neoandrographolide – functional groups in A. paniculata leaves of different accessions. The harvesting parameter was set at three different ages of 120, 150 and 180 days after transplanting (DAT) and at two different time sessions of morning (7:30–10:30 am) and evening (2:30–5.30 pm). RESULTS: OPLS-DA successfully discriminated the A. paniculata crude extracts into groups of which the main constituents – andrographolide and neoandrographolide – could be mainly observed in the morning session of 120 DAT for P and T, while H gave the highest intensities of these constituents at 150 DAT. CONCLUSION: The information extracted from ATR-FTIR data through OPLS-DA could be useful in tailoring this plant harvest stage in relation to the content of its two major diterpene lactones: andrographolide and neoandrographolide. John Wiley & Sons 2015-01-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/49725/1/Yusof_et_al_2015_%281%29.pdf Yusof, Nur A'thifah and Isha, Azizul and Ismail, Intan Safinar and Khatib, Alfi and Shaari, Khozirah and Abas, Faridah and Rukayadi, Yaya (2015) Infrared–metabolomics approach in detecting changes in andrographis paniculata metabolites due to different harvesting ages and times. Journal of the Science of Food and Agriculture, 95 (12). pp. 2533-2543. ISSN 0022-5142 E-ISSN 1097-0010 http://onlinelibrary.wiley.com/doi/10.1002/jsfa.6987/abstract;jsessionid=B678E300FD5668C13528DE1822BE140D.f01t03?systemMessage=Wiley+Online+Library+will+be+unavailable+on+Saturday+27th+February+from+09%3A00-14%3A00+GMT+%2F+04%3A00-09%3A00+EST+%2F+17%3A00- 10.1002/jsfa.6987
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Yusof, Nur A'thifah Isha, Azizul Ismail, Intan Safinar Khatib, Alfi Shaari, Khozirah Abas, Faridah Rukayadi, Yaya Infrared–metabolomics approach in detecting changes in andrographis paniculata metabolites due to different harvesting ages and times
description	BACKGROUND: The metabolite changes in three germplasm accessions of Malaysia Andrographis paniculata (Burm. F.) Nees, viz. 11265 (H), 11341 (P) and 11248 (T), due to their different harvesting ages and times were successfully evaluated by attenuated total reflectance (ATR)–Fourier transform infrared (FTIR) spectroscopy and translated through multivariate data analysis of principal component analysis (PCA) and orthogonal partial least square–discriminant analysis (OPLS-DA). This present study revealed the feasibility of ATR-FTIR in detecting the trend changes of the major metabolites – andrographolide and neoandrographolide – functional groups in A. paniculata leaves of different accessions. The harvesting parameter was set at three different ages of 120, 150 and 180 days after transplanting (DAT) and at two different time sessions of morning (7:30–10:30 am) and evening (2:30–5.30 pm). RESULTS: OPLS-DA successfully discriminated the A. paniculata crude extracts into groups of which the main constituents – andrographolide and neoandrographolide – could be mainly observed in the morning session of 120 DAT for P and T, while H gave the highest intensities of these constituents at 150 DAT. CONCLUSION: The information extracted from ATR-FTIR data through OPLS-DA could be useful in tailoring this plant harvest stage in relation to the content of its two major diterpene lactones: andrographolide and neoandrographolide.
format	Article
author	Yusof, Nur A'thifah Isha, Azizul Ismail, Intan Safinar Khatib, Alfi Shaari, Khozirah Abas, Faridah Rukayadi, Yaya
author_facet	Yusof, Nur A'thifah Isha, Azizul Ismail, Intan Safinar Khatib, Alfi Shaari, Khozirah Abas, Faridah Rukayadi, Yaya
author_sort	Yusof, Nur A'thifah
title	Infrared–metabolomics approach in detecting changes in andrographis paniculata metabolites due to different harvesting ages and times
title_short	Infrared–metabolomics approach in detecting changes in andrographis paniculata metabolites due to different harvesting ages and times
title_full	Infrared–metabolomics approach in detecting changes in andrographis paniculata metabolites due to different harvesting ages and times
title_fullStr	Infrared–metabolomics approach in detecting changes in andrographis paniculata metabolites due to different harvesting ages and times
title_full_unstemmed	Infrared–metabolomics approach in detecting changes in andrographis paniculata metabolites due to different harvesting ages and times
title_sort	infrared–metabolomics approach in detecting changes in andrographis paniculata metabolites due to different harvesting ages and times
publisher	John Wiley & Sons
publishDate	2015
url	http://irep.iium.edu.my/49725/ http://irep.iium.edu.my/49725/ http://irep.iium.edu.my/49725/ http://irep.iium.edu.my/49725/1/Yusof_et_al_2015_%281%29.pdf
first_indexed	2023-09-18T21:10:16Z
last_indexed	2023-09-18T21:10:16Z
_version_	1777411205711790080

Infrared–metabolomics approach in detecting changes in andrographis paniculata metabolites due to different harvesting ages and times

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