Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase

Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase

This procedure is following Gulati, Saxena, and Gupta (1997) protocol which is known as semi-quantitative plate assay for screening L-asparaginase- producing bacterial from local hot spring. The plate agar medium containing asparagine (as sole nitrogen sourse) is prepared with addition of phenol red...

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Main Authors: Jimat, Dzun Noraini, Mohamed , Intan Baizura Firda
Other Authors: Jamal, Parveen
Format: Book Chapter
Language:English
Published: IIUM Press 2016
Subjects:
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/49762/
http://irep.iium.edu.my/49762/
http://irep.iium.edu.my/49762/1/49762_Isolation_and_characterisation_of_thermophilic_bacterial_isolates_producing_L-asparaginase.pdf
id iium-49762
recordtype eprints
spelling iium-497622017-01-16T06:23:28Z http://irep.iium.edu.my/49762/ Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase Jimat, Dzun Noraini Mohamed , Intan Baizura Firda TP248.13 Biotechnology This procedure is following Gulati, Saxena, and Gupta (1997) protocol which is known as semi-quantitative plate assay for screening L-asparaginase- producing bacterial from local hot spring. The plate agar medium containing asparagine (as sole nitrogen sourse) is prepared with addition of phenol red which later shows an indication of L-asparaginase based on changes of colour. Phenol red at acidic is yellow which might change colour to pink when it is at alkaline. Thus, it indicates the formation of pink zone around microbial colonies producing L-asparaginase. The inoculated agar plates are incubated at 370C in an incubator for 24 hours. Thus, the isolation and screening of bacterial producing L-asparaginase are based on phenol-red zone. After purification, the isolates are characterised using several biochemical tests. IIUM Press Jamal, Parveen Azmi, Azlin Suhaida Ibrahim Ali , Noorbatcha 2016-01 Book Chapter NonPeerReviewed application/pdf en http://irep.iium.edu.my/49762/1/49762_Isolation_and_characterisation_of_thermophilic_bacterial_isolates_producing_L-asparaginase.pdf Jimat, Dzun Noraini and Mohamed , Intan Baizura Firda (2016) Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase. In: Experimental Methods in Modern Biotechnology. IIUM Press Series of Textbook from the Islamic Perspectives, 2 . IIUM Press, Kuala Lumpur, pp. 72-80. ISBN 978-967-418-384-4 http://iiumpress.iium.edu.my/bookshop/experimental-methods-in-modern-biotechnology
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Jimat, Dzun Noraini
Mohamed , Intan Baizura Firda
Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase
description This procedure is following Gulati, Saxena, and Gupta (1997) protocol which is known as semi-quantitative plate assay for screening L-asparaginase- producing bacterial from local hot spring. The plate agar medium containing asparagine (as sole nitrogen sourse) is prepared with addition of phenol red which later shows an indication of L-asparaginase based on changes of colour. Phenol red at acidic is yellow which might change colour to pink when it is at alkaline. Thus, it indicates the formation of pink zone around microbial colonies producing L-asparaginase. The inoculated agar plates are incubated at 370C in an incubator for 24 hours. Thus, the isolation and screening of bacterial producing L-asparaginase are based on phenol-red zone. After purification, the isolates are characterised using several biochemical tests.
author2 Jamal, Parveen
author_facet Jamal, Parveen
Jimat, Dzun Noraini
Mohamed , Intan Baizura Firda
format Book Chapter
author Jimat, Dzun Noraini
Mohamed , Intan Baizura Firda
author_sort Jimat, Dzun Noraini
title Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase
title_short Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase
title_full Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase
title_fullStr Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase
title_full_unstemmed Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase
title_sort isolation and characterisation of thermophilic bacterial isolates producing l-asparaginase
publisher IIUM Press
publishDate 2016
url http://irep.iium.edu.my/49762/
http://irep.iium.edu.my/49762/
http://irep.iium.edu.my/49762/1/49762_Isolation_and_characterisation_of_thermophilic_bacterial_isolates_producing_L-asparaginase.pdf
first_indexed 2023-09-18T21:10:19Z
last_indexed 2023-09-18T21:10:19Z
_version_ 1777411209137487872

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