Correlation between HBSAG quantification and HBV genotypesin chronic HBV infections

Accumulated data has been proposed that viral genotypes and sub genotypesplay an important role in clinical outcome and antiviral monitoring of chronic HBV infection. Genotyping and quantitating of HBV DNA levels molecularly is relatively expensive. On the other side, serum HBsAg quantification has...

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Main Authors:	Hadi, Qabas Naemah, Mahmud, Mohammed Imad A. Mustafa, Chee, Hui Yee, Jaafar, Khairul Azhar
Format:	Article
Language:	English
Published:	Institute of Research and Journals 2015
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/50015/ http://irep.iium.edu.my/50015/ http://irep.iium.edu.my/50015/1/Imad_Correlation_Hep_B_IJASEAT-CHY.pdf

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spelling	iium-500152017-01-12T06:33:41Z http://irep.iium.edu.my/50015/ Correlation between HBSAG quantification and HBV genotypesin chronic HBV infections Hadi, Qabas Naemah Mahmud, Mohammed Imad A. Mustafa Chee, Hui Yee Jaafar, Khairul Azhar R Medicine (General) Accumulated data has been proposed that viral genotypes and sub genotypesplay an important role in clinical outcome and antiviral monitoring of chronic HBV infection. Genotyping and quantitating of HBV DNA levels molecularly is relatively expensive. On the other side, serum HBsAg quantification has been recently standardized by automated quantitative assays leading to revived interest in the clinical utilization of this marker for diagnosis using sensitive and reliable commercial assays. Several studies have referred to the kinetics of HBsAg to predict response to antiviral therapy. Thus, a cheaper laboratory test as a surrogate diagnostic marker might simplify patients’ management. Objectives: to evaluate whether quantitative HBsAg levels correlate with HBV genotypes in Malaysian CHB patients. Patients and Methods: In this cross-sectional study, fifty serum samples from patients with hepatitis B were used, however those tested positive for antibodies to HCV and HDV were excluded. The serum HBsAg level was quantified by Elecsysassay. In addition, HBV DNA load was measured by real-time polymerase chain reaction whereas identification of HBV genotypes was done by direct sequencing. Results: Of 50 patients, 31 were males (44%) and 19 females (27%); the mean age was37 ± 12 years. Forty-two (42) patients had HBsAg level >100 IU/mL.Eightypercent (40) were HBeAg-negative while 20% (10) were positive. Genotyping showed that 22 patients had genotype C (44%), and 18 patients were of genotype B (36%). Applying Mann-Whitney test, HBs Agtiters correlated differentially with HBV genotypes C& B. (p<0.05). By Nonparametric T test, there was no significant correlation between HBsAg levels and HBV DNA levels (p<0.05).Conclusions: the statistically significant association between levels of HBsAg and HBV genotypes was observed especially for genotype C. Hence, HBsAg level could be a useful serological marker to predict the genotypes B and C of HBV and thus possibly anticipate the response to treatment with antiviral drugs. Institute of Research and Journals 2015-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/50015/1/Imad_Correlation_Hep_B_IJASEAT-CHY.pdf Hadi, Qabas Naemah and Mahmud, Mohammed Imad A. Mustafa and Chee, Hui Yee and Jaafar, Khairul Azhar (2015) Correlation between HBSAG quantification and HBV genotypesin chronic HBV infections. International Journal of Advances in Science, Engineering and Technology (IJASEAT), 3 (2). pp. 1-6. ISSN 2321 –8991 E-ISSN 2321 –9009 http://www.iraj.in/journal/journal_file/journal_pdf/6-128-14301326671-5.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Hadi, Qabas Naemah Mahmud, Mohammed Imad A. Mustafa Chee, Hui Yee Jaafar, Khairul Azhar Correlation between HBSAG quantification and HBV genotypesin chronic HBV infections
description	Accumulated data has been proposed that viral genotypes and sub genotypesplay an important role in clinical outcome and antiviral monitoring of chronic HBV infection. Genotyping and quantitating of HBV DNA levels molecularly is relatively expensive. On the other side, serum HBsAg quantification has been recently standardized by automated quantitative assays leading to revived interest in the clinical utilization of this marker for diagnosis using sensitive and reliable commercial assays. Several studies have referred to the kinetics of HBsAg to predict response to antiviral therapy. Thus, a cheaper laboratory test as a surrogate diagnostic marker might simplify patients’ management. Objectives: to evaluate whether quantitative HBsAg levels correlate with HBV genotypes in Malaysian CHB patients. Patients and Methods: In this cross-sectional study, fifty serum samples from patients with hepatitis B were used, however those tested positive for antibodies to HCV and HDV were excluded. The serum HBsAg level was quantified by Elecsysassay. In addition, HBV DNA load was measured by real-time polymerase chain reaction whereas identification of HBV genotypes was done by direct sequencing. Results: Of 50 patients, 31 were males (44%) and 19 females (27%); the mean age was37 ± 12 years. Forty-two (42) patients had HBsAg level >100 IU/mL.Eightypercent (40) were HBeAg-negative while 20% (10) were positive. Genotyping showed that 22 patients had genotype C (44%), and 18 patients were of genotype B (36%). Applying Mann-Whitney test, HBs Agtiters correlated differentially with HBV genotypes C& B. (p<0.05). By Nonparametric T test, there was no significant correlation between HBsAg levels and HBV DNA levels (p<0.05).Conclusions: the statistically significant association between levels of HBsAg and HBV genotypes was observed especially for genotype C. Hence, HBsAg level could be a useful serological marker to predict the genotypes B and C of HBV and thus possibly anticipate the response to treatment with antiviral drugs.
format	Article
author	Hadi, Qabas Naemah Mahmud, Mohammed Imad A. Mustafa Chee, Hui Yee Jaafar, Khairul Azhar
author_facet	Hadi, Qabas Naemah Mahmud, Mohammed Imad A. Mustafa Chee, Hui Yee Jaafar, Khairul Azhar
author_sort	Hadi, Qabas Naemah
title	Correlation between HBSAG quantification and HBV genotypesin chronic HBV infections
title_short	Correlation between HBSAG quantification and HBV genotypesin chronic HBV infections
title_full	Correlation between HBSAG quantification and HBV genotypesin chronic HBV infections
title_fullStr	Correlation between HBSAG quantification and HBV genotypesin chronic HBV infections
title_full_unstemmed	Correlation between HBSAG quantification and HBV genotypesin chronic HBV infections
title_sort	correlation between hbsag quantification and hbv genotypesin chronic hbv infections
publisher	Institute of Research and Journals
publishDate	2015
url	http://irep.iium.edu.my/50015/ http://irep.iium.edu.my/50015/ http://irep.iium.edu.my/50015/1/Imad_Correlation_Hep_B_IJASEAT-CHY.pdf
first_indexed	2023-09-18T21:10:41Z
last_indexed	2023-09-18T21:10:41Z
_version_	1777411231721717760

Correlation between HBSAG quantification and HBV genotypesin chronic HBV infections

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