Identification of spermatogonial stem cell-like cell differentiation in vitro from azoospermia patient using modified human embryonic stem cell (HESC) media

Background:Spermatogenesis is a process where proliferation and differentiation of spermatogonial stem cell (SSC) into haploid sperm cells in the testis. Azoospermia patients have impaired spermatogenesis where they have difficulty to get normal sperm. The purpose of this study is to proliferate and...

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Main Authors:	Abdul Wahab, Azantee Yazmie, Md Isa, Muhammad Lokman, Ramli, Roszaman
Format:	Article
Language:	English
Published:	OMICS International 2015
Subjects:	RG Gynecology and obstetrics
Online Access:	http://irep.iium.edu.my/50364/ http://irep.iium.edu.my/50364/ http://irep.iium.edu.my/50364/ http://irep.iium.edu.my/50364/1/identification-of-spermatogonial-stem-celllike-cell-differentiation-in-vitrofrom-azoospermia-patient-using-modified-human-embryoni-2167-0250-1000145.pdf

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spelling	iium-503642016-07-15T06:49:20Z http://irep.iium.edu.my/50364/ Identification of spermatogonial stem cell-like cell differentiation in vitro from azoospermia patient using modified human embryonic stem cell (HESC) media Abdul Wahab, Azantee Yazmie Md Isa, Muhammad Lokman Ramli, Roszaman RG Gynecology and obstetrics Background:Spermatogenesis is a process where proliferation and differentiation of spermatogonial stem cell (SSC) into haploid sperm cells in the testis. Azoospermia patients have impaired spermatogenesis where they have difficulty to get normal sperm. The purpose of this study is to proliferate and differentiate SSC-like cell in in vitro culture from azoospermia patient samples and to determine the pattern of gene expressions involved. Methods and Results: Testicular biopsy tissue obtained from obstructive azoospermia (OA) and non-obstructive azoospermia (NOA) patients were dissociated and cultured in different human embryonic stem cell (HESC) media added with specific growth factors and reproductive hormones according to the day of culture using 24 well plate dishes. SSC-like cell colonies were formed after 5 weeks of culture. Gene expression analyses were done on day 49 and day 90 using quantitative reverse transcription polymerase chain reaction (q-RT PCR). OCT4 was detected in NOA patient and ITGB1 was identified in OA patient for 49 days of culture. Gene expression for all stages of spermatogenic cells in day 90 were mostly detected in OA patient; ITGB1, SCP3, H2B and TNP1. However, gene TNP1 was only expressed in NOA patient shown spermatid formation stage. Conclusions: Our result shown that there is a potential to develop sperm in vitro culture from testis biopsy of azoospermic patients for further clinical application OMICS International 2015-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/50364/1/identification-of-spermatogonial-stem-celllike-cell-differentiation-in-vitrofrom-azoospermia-patient-using-modified-human-embryoni-2167-0250-1000145.pdf Abdul Wahab, Azantee Yazmie and Md Isa, Muhammad Lokman and Ramli, Roszaman (2015) Identification of spermatogonial stem cell-like cell differentiation in vitro from azoospermia patient using modified human embryonic stem cell (HESC) media. Andrology (Los Angel), 4 (2). (1000145)1-5. ISSN 2167-0250 http://www.omicsgroup.org/journals/identification-of-spermatogonial-stem-celllike-cell-differentiation-in-vitrofrom-azoospermia-patient-using-modified-human-embryoni-2167-0250-1000145.pdf 10.4172/2167-0250.1000145
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RG Gynecology and obstetrics
spellingShingle	RG Gynecology and obstetrics Abdul Wahab, Azantee Yazmie Md Isa, Muhammad Lokman Ramli, Roszaman Identification of spermatogonial stem cell-like cell differentiation in vitro from azoospermia patient using modified human embryonic stem cell (HESC) media
description	Background:Spermatogenesis is a process where proliferation and differentiation of spermatogonial stem cell (SSC) into haploid sperm cells in the testis. Azoospermia patients have impaired spermatogenesis where they have difficulty to get normal sperm. The purpose of this study is to proliferate and differentiate SSC-like cell in in vitro culture from azoospermia patient samples and to determine the pattern of gene expressions involved. Methods and Results: Testicular biopsy tissue obtained from obstructive azoospermia (OA) and non-obstructive azoospermia (NOA) patients were dissociated and cultured in different human embryonic stem cell (HESC) media added with specific growth factors and reproductive hormones according to the day of culture using 24 well plate dishes. SSC-like cell colonies were formed after 5 weeks of culture. Gene expression analyses were done on day 49 and day 90 using quantitative reverse transcription polymerase chain reaction (q-RT PCR). OCT4 was detected in NOA patient and ITGB1 was identified in OA patient for 49 days of culture. Gene expression for all stages of spermatogenic cells in day 90 were mostly detected in OA patient; ITGB1, SCP3, H2B and TNP1. However, gene TNP1 was only expressed in NOA patient shown spermatid formation stage. Conclusions: Our result shown that there is a potential to develop sperm in vitro culture from testis biopsy of azoospermic patients for further clinical application
format	Article
author	Abdul Wahab, Azantee Yazmie Md Isa, Muhammad Lokman Ramli, Roszaman
author_facet	Abdul Wahab, Azantee Yazmie Md Isa, Muhammad Lokman Ramli, Roszaman
author_sort	Abdul Wahab, Azantee Yazmie
title	Identification of spermatogonial stem cell-like cell differentiation in vitro from azoospermia patient using modified human embryonic stem cell (HESC) media
title_short	Identification of spermatogonial stem cell-like cell differentiation in vitro from azoospermia patient using modified human embryonic stem cell (HESC) media
title_full	Identification of spermatogonial stem cell-like cell differentiation in vitro from azoospermia patient using modified human embryonic stem cell (HESC) media
title_fullStr	Identification of spermatogonial stem cell-like cell differentiation in vitro from azoospermia patient using modified human embryonic stem cell (HESC) media
title_full_unstemmed	Identification of spermatogonial stem cell-like cell differentiation in vitro from azoospermia patient using modified human embryonic stem cell (HESC) media
title_sort	identification of spermatogonial stem cell-like cell differentiation in vitro from azoospermia patient using modified human embryonic stem cell (hesc) media
publisher	OMICS International
publishDate	2015
url	http://irep.iium.edu.my/50364/ http://irep.iium.edu.my/50364/ http://irep.iium.edu.my/50364/ http://irep.iium.edu.my/50364/1/identification-of-spermatogonial-stem-celllike-cell-differentiation-in-vitrofrom-azoospermia-patient-using-modified-human-embryoni-2167-0250-1000145.pdf
first_indexed	2023-09-18T21:11:11Z
last_indexed	2023-09-18T21:11:11Z
_version_	1777411263003885568

Identification of spermatogonial stem cell-like cell differentiation in vitro from azoospermia patient using modified human embryonic stem cell (HESC) media

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