Statistical optimization of process conditions for cellulase production by liquid state bioconversion of domestic wastewater sludge

A two-level fractional factorial design (FFD) was used to determine the effects of six factors, i.e. substrate (domestic wastewater sludge – DWS) and co-substrate concentration (wheat flour – WF), temperature, initial pH, inoculum size and agitation rate on the production of cellulase enzyme by Tric...

Full description

Bibliographic Details
Main Authors:	Alam, Md. Zahangir, Muyibi, Suleyman Aremu, Wahid, Rosmaziah
Format:	Article
Language:	English
Published:	Elsevier 2008
Subjects:	TP Chemical technology TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/5041/ http://irep.iium.edu.my/5041/ http://irep.iium.edu.my/5041/ http://irep.iium.edu.my/5041/1/Paper-2008-BT.pdf

id	iium-5041
recordtype	eprints
spelling	iium-50412013-07-16T05:56:42Z http://irep.iium.edu.my/5041/ Statistical optimization of process conditions for cellulase production by liquid state bioconversion of domestic wastewater sludge Alam, Md. Zahangir Muyibi, Suleyman Aremu Wahid, Rosmaziah TP Chemical technology TP248.13 Biotechnology A two-level fractional factorial design (FFD) was used to determine the effects of six factors, i.e. substrate (domestic wastewater sludge – DWS) and co-substrate concentration (wheat flour – WF), temperature, initial pH, inoculum size and agitation rate on the production of cellulase enzyme by Trichoderma harzianum in liquid state bioconversion. On statistical analysis of the results from the experimental studies, optimum process conditions were found to be temperature 32.5 �C, substrate concentration (DWS) 0.75% (w/w), co-substrate (WF) concentration 2% (w/w), initial pH 5, inoculum size 2% (v/w) and agitation 175 rpm. Analysis of variance (ANOVA) showed a high coefficient of determination (R2) of 0.975. Cellulase activity reached 10.2 FPU/ml at day 3 during the fermentation process which indicated about 1.5-fold increase in production compared to the cellulase activity obtained from the results of design of experiment (6.9 FPU/ml). Biodegradation of DWS was also evaluated to verify the efficiency of the bioconversion process as a waste management method. Elsevier 2008 Article PeerReviewed application/pdf en http://irep.iium.edu.my/5041/1/Paper-2008-BT.pdf Alam, Md. Zahangir and Muyibi, Suleyman Aremu and Wahid, Rosmaziah (2008) Statistical optimization of process conditions for cellulase production by liquid state bioconversion of domestic wastewater sludge. Bioresource Technology, 99 (11). pp. 4709-4716. ISSN 0960-8524 http://dx.doi.org/10.1016/j.biortech.2007.09.072 doi:10.1016/j.biortech.2007.09.072
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP Chemical technology TP248.13 Biotechnology
spellingShingle	TP Chemical technology TP248.13 Biotechnology Alam, Md. Zahangir Muyibi, Suleyman Aremu Wahid, Rosmaziah Statistical optimization of process conditions for cellulase production by liquid state bioconversion of domestic wastewater sludge
description	A two-level fractional factorial design (FFD) was used to determine the effects of six factors, i.e. substrate (domestic wastewater sludge – DWS) and co-substrate concentration (wheat flour – WF), temperature, initial pH, inoculum size and agitation rate on the production of cellulase enzyme by Trichoderma harzianum in liquid state bioconversion. On statistical analysis of the results from the experimental studies, optimum process conditions were found to be temperature 32.5 �C, substrate concentration (DWS) 0.75% (w/w), co-substrate (WF) concentration 2% (w/w), initial pH 5, inoculum size 2% (v/w) and agitation 175 rpm. Analysis of variance (ANOVA) showed a high coefficient of determination (R2) of 0.975. Cellulase activity reached 10.2 FPU/ml at day 3 during the fermentation process which indicated about 1.5-fold increase in production compared to the cellulase activity obtained from the results of design of experiment (6.9 FPU/ml). Biodegradation of DWS was also evaluated to verify the efficiency of the bioconversion process as a waste management method.
format	Article
author	Alam, Md. Zahangir Muyibi, Suleyman Aremu Wahid, Rosmaziah
author_facet	Alam, Md. Zahangir Muyibi, Suleyman Aremu Wahid, Rosmaziah
author_sort	Alam, Md. Zahangir
title	Statistical optimization of process conditions for cellulase production by liquid state bioconversion of domestic wastewater sludge
title_short	Statistical optimization of process conditions for cellulase production by liquid state bioconversion of domestic wastewater sludge
title_full	Statistical optimization of process conditions for cellulase production by liquid state bioconversion of domestic wastewater sludge
title_fullStr	Statistical optimization of process conditions for cellulase production by liquid state bioconversion of domestic wastewater sludge
title_full_unstemmed	Statistical optimization of process conditions for cellulase production by liquid state bioconversion of domestic wastewater sludge
title_sort	statistical optimization of process conditions for cellulase production by liquid state bioconversion of domestic wastewater sludge
publisher	Elsevier
publishDate	2008
url	http://irep.iium.edu.my/5041/ http://irep.iium.edu.my/5041/ http://irep.iium.edu.my/5041/ http://irep.iium.edu.my/5041/1/Paper-2008-BT.pdf
first_indexed	2023-09-18T20:13:31Z
last_indexed	2023-09-18T20:13:31Z
_version_	1777407635444727808

Statistical optimization of process conditions for cellulase production by liquid state bioconversion of domestic wastewater sludge

Similar Items