Bioconversion of palm oil mill effluent for citric acid production: statistical optimization of fermentation media and time by central composite design

A laboratory-scale study was conducted to evaluate the feasibility of using palm oil mill effluent (POME) as a major substrate and other nutrients for maximum production of citric acid using the potential fungal strain Aspergillus niger (A103). Statistical optimization of medium composition (subst...

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Main Authors:	Alam, Md. Zahangir, Jamal, Parveen, M. Nadzir, Masrina
Format:	Article
Language:	English
Published:	Springer Science 2008
Subjects:	TP Chemical technology TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/5042/ http://irep.iium.edu.my/5042/ http://irep.iium.edu.my/5042/ http://irep.iium.edu.my/5042/1/Paper-2008-WJMB.pdf

id	iium-5042
recordtype	eprints
spelling	iium-50422013-07-16T06:00:10Z http://irep.iium.edu.my/5042/ Bioconversion of palm oil mill effluent for citric acid production: statistical optimization of fermentation media and time by central composite design Alam, Md. Zahangir Jamal, Parveen M. Nadzir, Masrina TP Chemical technology TP248.13 Biotechnology A laboratory-scale study was conducted to evaluate the feasibility of using palm oil mill effluent (POME) as a major substrate and other nutrients for maximum production of citric acid using the potential fungal strain Aspergillus niger (A103). Statistical optimization of medium composition (substrate–POME,co-substrates–wheat flour and glucose, and nitrogen source–ammonium nitrate) and fermentation time was carried out by central composite design (CCD) to develop a polynomial regression model through the effects of linear,quadratic, and interaction of the factors. The statistical analysis of the results showed that, in the range studied, ammonium nitrate had no significant effect whereas substrate,co-substrates and fermentation time had significant effects on citric acid production. The optimized medium containing 2% (w/w) of substrate concentration (POME), 4% (w/w) of wheat flour concentration, 4% (w/w) of glucose concentration, 0% (w/v) of ammonium nitrate and 5 days fermentation time gave the maximum predicted citric acid of 5.37 g/l which was found to be 1.5 g/l in the experimental run. The determination of coefficient (R2) from the analysis observed was 0.964, indicating a satisfactory adjustment of the model with the response. The analysis showed that the major substrate POME (P\0.05), glucose (P\0.01), nutrient (P\0.05), and fermentation time (P\0.01) was more significant for citric acid production. The bioconversion of POME for citric acid production using optimal conditions showed the higher removal of chemical oxygen demand (82%) with the production of citric acid (5.2 g/l) on the final day of fermentation process (7 days). The pH and biosolids accumulation were observed during the bioconversion process. Springer Science 2008 Article PeerReviewed application/pdf en http://irep.iium.edu.my/5042/1/Paper-2008-WJMB.pdf Alam, Md. Zahangir and Jamal, Parveen and M. Nadzir, Masrina (2008) Bioconversion of palm oil mill effluent for citric acid production: statistical optimization of fermentation media and time by central composite design. World Journal of Microbiology and Biotechnology, 24 (7). pp. 1177-1185. ISSN 0959-3993 http://dx.doi.org/10.1007/s11274-007-9590-5 doi:10.1007/s11274-007-9590-5
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP Chemical technology TP248.13 Biotechnology
spellingShingle	TP Chemical technology TP248.13 Biotechnology Alam, Md. Zahangir Jamal, Parveen M. Nadzir, Masrina Bioconversion of palm oil mill effluent for citric acid production: statistical optimization of fermentation media and time by central composite design
description	A laboratory-scale study was conducted to evaluate the feasibility of using palm oil mill effluent (POME) as a major substrate and other nutrients for maximum production of citric acid using the potential fungal strain Aspergillus niger (A103). Statistical optimization of medium composition (substrate–POME,co-substrates–wheat flour and glucose, and nitrogen source–ammonium nitrate) and fermentation time was carried out by central composite design (CCD) to develop a polynomial regression model through the effects of linear,quadratic, and interaction of the factors. The statistical analysis of the results showed that, in the range studied, ammonium nitrate had no significant effect whereas substrate,co-substrates and fermentation time had significant effects on citric acid production. The optimized medium containing 2% (w/w) of substrate concentration (POME), 4% (w/w) of wheat flour concentration, 4% (w/w) of glucose concentration, 0% (w/v) of ammonium nitrate and 5 days fermentation time gave the maximum predicted citric acid of 5.37 g/l which was found to be 1.5 g/l in the experimental run. The determination of coefficient (R2) from the analysis observed was 0.964, indicating a satisfactory adjustment of the model with the response. The analysis showed that the major substrate POME (P\0.05), glucose (P\0.01), nutrient (P\0.05), and fermentation time (P\0.01) was more significant for citric acid production. The bioconversion of POME for citric acid production using optimal conditions showed the higher removal of chemical oxygen demand (82%) with the production of citric acid (5.2 g/l) on the final day of fermentation process (7 days). The pH and biosolids accumulation were observed during the bioconversion process.
format	Article
author	Alam, Md. Zahangir Jamal, Parveen M. Nadzir, Masrina
author_facet	Alam, Md. Zahangir Jamal, Parveen M. Nadzir, Masrina
author_sort	Alam, Md. Zahangir
title	Bioconversion of palm oil mill effluent for citric acid production: statistical optimization of fermentation media and time by central composite design
title_short	Bioconversion of palm oil mill effluent for citric acid production: statistical optimization of fermentation media and time by central composite design
title_full	Bioconversion of palm oil mill effluent for citric acid production: statistical optimization of fermentation media and time by central composite design
title_fullStr	Bioconversion of palm oil mill effluent for citric acid production: statistical optimization of fermentation media and time by central composite design
title_full_unstemmed	Bioconversion of palm oil mill effluent for citric acid production: statistical optimization of fermentation media and time by central composite design
title_sort	bioconversion of palm oil mill effluent for citric acid production: statistical optimization of fermentation media and time by central composite design
publisher	Springer Science
publishDate	2008
url	http://irep.iium.edu.my/5042/ http://irep.iium.edu.my/5042/ http://irep.iium.edu.my/5042/ http://irep.iium.edu.my/5042/1/Paper-2008-WJMB.pdf
first_indexed	2023-09-18T20:13:31Z
last_indexed	2023-09-18T20:13:31Z
_version_	1777407635592577024

Bioconversion of palm oil mill effluent for citric acid production: statistical optimization of fermentation media and time by central composite design

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