Solid state bioconversion of oil palm empty fruit bunches for cellulase enzyme production using a rotary drum bioreactor
Bioconversion of oil palm lignocellulosic biomass in the form of empty fruit bunches (EFB) as the major substrate into cellulase enzyme was studied in the laboratory. A fungal strain, Trichoderma harzianum T2008 was used to evaluate the solid state bioconversion of EFB for cellulase production. The...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier Science
2009
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/5058/ http://irep.iium.edu.my/5058/ http://irep.iium.edu.my/5058/ http://irep.iium.edu.my/5058/1/Cellulase-solid-state-BEJ.pdf |
| Summary: | Bioconversion of oil palm lignocellulosic biomass in the form of empty fruit bunches (EFB) as the major substrate into cellulase enzyme was studied in the laboratory. A fungal strain, Trichoderma harzianum T2008 was used to evaluate the solid state bioconversion of EFB for cellulase production. The study was conducted in two systems: an Erlenmeyer flask (EF, 500 mL) and a horizontal rotary drum bioreactor (RDB, 50 L), designed and fabricated locally. The highest cellulase activity on the fourth day of fermentation in the EF was 8.2 filter paper activity (FPA)/gram dry solids (gds) of EFB, while its activity from the RDB was 10.1 FPA/gds on the second day of fermentation. Glucosamine, reducing sugar and pH were also
determined to evaluate the substrate uptake and growth conditions during the entire fermentation period. |
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