In silico design in designing xylanase for biobleaching industry

The use of hemicellulolytic enzymes has recently attracted considerable interest as a substitute for chlorine chemicals in pulp bleaching. The challenges in incorporating enzyme to the bleaching system are to have a stable and active xylanase at high temperatures and higher alkaline pH conditions. I...

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Main Authors:	Noorbatcha, Ibrahim Ali, Abdul Hadi, Muaz, Ismail, Ahmad Faris, Mohd. Salleh, Hamzah
Format:	Article
Language:	English
Published:	Asian Network for Scientific Information 2009
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/506/ http://irep.iium.edu.my/506/ http://irep.iium.edu.my/506/1/3184-3187.pdf

id	iium-506
recordtype	eprints
spelling	iium-5062011-11-16T02:21:56Z http://irep.iium.edu.my/506/ In silico design in designing xylanase for biobleaching industry Noorbatcha, Ibrahim Ali Abdul Hadi, Muaz Ismail, Ahmad Faris Mohd. Salleh, Hamzah TP248.13 Biotechnology The use of hemicellulolytic enzymes has recently attracted considerable interest as a substitute for chlorine chemicals in pulp bleaching. The challenges in incorporating enzyme to the bleaching system are to have a stable and active xylanase at high temperatures and higher alkaline pH conditions. In this study, we have used computational methods to analyze the structural factors responsible for the activity and themostability of Bacillus circulans xylanase which have been identified as one of the promising xylanase source to treat the pulp before bleaching it through the conventional bleaching sequences. Simulated point mutation shows that arginine substitution potentially increased the number of hydrogen bond; correlate with the xylanase activity and some changes in amino acid sequence specifically to the α-helix and β-sheet appeared to be promising in improving thermostability of xylanase. This in silico method results can be used to develop new efficient xylanase for pulp bleaching industry. Asian Network for Scientific Information 2009 Article PeerReviewed application/pdf en http://irep.iium.edu.my/506/1/3184-3187.pdf Noorbatcha, Ibrahim Ali and Abdul Hadi, Muaz and Ismail, Ahmad Faris and Mohd. Salleh, Hamzah (2009) In silico design in designing xylanase for biobleaching industry. Journal of Applied Sciences, 9 (17). pp. 3184-3187. ISSN 1812-5662 (O), 1812-5654 (P) http://www.ansinet.com/current.php?issn=1812-5654
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Noorbatcha, Ibrahim Ali Abdul Hadi, Muaz Ismail, Ahmad Faris Mohd. Salleh, Hamzah In silico design in designing xylanase for biobleaching industry
description	The use of hemicellulolytic enzymes has recently attracted considerable interest as a substitute for chlorine chemicals in pulp bleaching. The challenges in incorporating enzyme to the bleaching system are to have a stable and active xylanase at high temperatures and higher alkaline pH conditions. In this study, we have used computational methods to analyze the structural factors responsible for the activity and themostability of Bacillus circulans xylanase which have been identified as one of the promising xylanase source to treat the pulp before bleaching it through the conventional bleaching sequences. Simulated point mutation shows that arginine substitution potentially increased the number of hydrogen bond; correlate with the xylanase activity and some changes in amino acid sequence specifically to the α-helix and β-sheet appeared to be promising in improving thermostability of xylanase. This in silico method results can be used to develop new efficient xylanase for pulp bleaching industry.
format	Article
author	Noorbatcha, Ibrahim Ali Abdul Hadi, Muaz Ismail, Ahmad Faris Mohd. Salleh, Hamzah
author_facet	Noorbatcha, Ibrahim Ali Abdul Hadi, Muaz Ismail, Ahmad Faris Mohd. Salleh, Hamzah
author_sort	Noorbatcha, Ibrahim Ali
title	In silico design in designing xylanase for biobleaching industry
title_short	In silico design in designing xylanase for biobleaching industry
title_full	In silico design in designing xylanase for biobleaching industry
title_fullStr	In silico design in designing xylanase for biobleaching industry
title_full_unstemmed	In silico design in designing xylanase for biobleaching industry
title_sort	in silico design in designing xylanase for biobleaching industry
publisher	Asian Network for Scientific Information
publishDate	2009
url	http://irep.iium.edu.my/506/ http://irep.iium.edu.my/506/ http://irep.iium.edu.my/506/1/3184-3187.pdf
first_indexed	2023-09-18T20:07:37Z
last_indexed	2023-09-18T20:07:37Z
_version_	1777407264353681408

In silico design in designing xylanase for biobleaching industry

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