IVF-ICSI split insemination reveals those cases of unexplained infertility benefitting from ICSI even when the DNA Fragmentation index is reduced to 15% or even 5%

The aim of the study was to assess the fertilization rate (FR) of randomized sibling oocytes inseminated by conventional IVF or ICSI in couples with unexplained infertility. Methods The 16-month study was conducted at an established private IVF facility. Oocytes recovered from couples with normal se...

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Main Authors:	Mustafa, Kamarul Bahyah, Yovich, John L, Marjanovich, Nicole, Yovich, Steven J
Format:	Article
Language:	English
Published:	Andrology & Gynecology: Current Research 2016
Subjects:	RG Gynecology and obstetrics
Online Access:	http://irep.iium.edu.my/50707/ http://irep.iium.edu.my/50707/ http://irep.iium.edu.my/50707/ http://irep.iium.edu.my/50707/3/50707.pdf

id	iium-50707
recordtype	eprints
spelling	iium-507072016-07-25T05:17:29Z http://irep.iium.edu.my/50707/ IVF-ICSI split insemination reveals those cases of unexplained infertility benefitting from ICSI even when the DNA Fragmentation index is reduced to 15% or even 5% Mustafa, Kamarul Bahyah Yovich, John L Marjanovich, Nicole Yovich, Steven J RG Gynecology and obstetrics The aim of the study was to assess the fertilization rate (FR) of randomized sibling oocytes inseminated by conventional IVF or ICSI in couples with unexplained infertility. Methods The 16-month study was conducted at an established private IVF facility. Oocytes recovered from couples with normal semen parameters and normal DNA fragmentation index (DFI; <30%), were randomly allocated to IVF or ICSI and the FR (2PN/MII) was assessed. Pregnancy outcome following embryo transfers were analyzed with regards to either IVF-embryo vs. ICSI-embryo, and in relation to DFI levels. Results Of 585 oocytes retrieved from 38 patients, 463 were mature (MII). The ICSI group generated a significantly higher number of 2PN embryos with a mean FR of 83.4% vs. 67.6% (p<0.05). There were no cases of complete fertilization failure (CFF) in the ICSI group, but there were 7.9% in the IVF group. The significant difference of FR was observed only when the DFI level was ≥ 15% and if such cutoff was applied, the CFF cases would be reduced to 2.6%. Of the 30 patients who had fresh embryo transfers performed, the ICSI group showed a higher pregnancy rate (69.2% vs. 58.8%; N.S.) with a significantly higher mean DFI value in the non-pregnant group (p<0.05). Conclusions IVF-ICSI split insemination can reveal those cases which will benefit from ICSI even where semen parameters and DFI are normal; however if the DFI is reduced to a 15% cut-off level, the rate of CFF will be minimized, but not completely excluded, even at 5%.. Andrology & Gynecology: Current Research 2016 Article PeerReviewed application/pdf en http://irep.iium.edu.my/50707/3/50707.pdf Mustafa, Kamarul Bahyah and Yovich, John L and Marjanovich, Nicole and Yovich, Steven J (2016) IVF-ICSI split insemination reveals those cases of unexplained infertility benefitting from ICSI even when the DNA Fragmentation index is reduced to 15% or even 5%. Andrology & Gynecology: Current Research, 4 (1). pp. 1-7. E-ISSN 2327-4360 http://www.scitechnol.com/peer-review/ivficsi-split-insemination-reveals-those-cases-of-unexplained-infertilitybenefitting-from-icsi-even-when-the-dna-fragmentation-ind-bV9X.php?article_id=4838 10.4172/2327-4360.1000145
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RG Gynecology and obstetrics
spellingShingle	RG Gynecology and obstetrics Mustafa, Kamarul Bahyah Yovich, John L Marjanovich, Nicole Yovich, Steven J IVF-ICSI split insemination reveals those cases of unexplained infertility benefitting from ICSI even when the DNA Fragmentation index is reduced to 15% or even 5%
description	The aim of the study was to assess the fertilization rate (FR) of randomized sibling oocytes inseminated by conventional IVF or ICSI in couples with unexplained infertility. Methods The 16-month study was conducted at an established private IVF facility. Oocytes recovered from couples with normal semen parameters and normal DNA fragmentation index (DFI; <30%), were randomly allocated to IVF or ICSI and the FR (2PN/MII) was assessed. Pregnancy outcome following embryo transfers were analyzed with regards to either IVF-embryo vs. ICSI-embryo, and in relation to DFI levels. Results Of 585 oocytes retrieved from 38 patients, 463 were mature (MII). The ICSI group generated a significantly higher number of 2PN embryos with a mean FR of 83.4% vs. 67.6% (p<0.05). There were no cases of complete fertilization failure (CFF) in the ICSI group, but there were 7.9% in the IVF group. The significant difference of FR was observed only when the DFI level was ≥ 15% and if such cutoff was applied, the CFF cases would be reduced to 2.6%. Of the 30 patients who had fresh embryo transfers performed, the ICSI group showed a higher pregnancy rate (69.2% vs. 58.8%; N.S.) with a significantly higher mean DFI value in the non-pregnant group (p<0.05). Conclusions IVF-ICSI split insemination can reveal those cases which will benefit from ICSI even where semen parameters and DFI are normal; however if the DFI is reduced to a 15% cut-off level, the rate of CFF will be minimized, but not completely excluded, even at 5%..
format	Article
author	Mustafa, Kamarul Bahyah Yovich, John L Marjanovich, Nicole Yovich, Steven J
author_facet	Mustafa, Kamarul Bahyah Yovich, John L Marjanovich, Nicole Yovich, Steven J
author_sort	Mustafa, Kamarul Bahyah
title	IVF-ICSI split insemination reveals those cases of unexplained infertility benefitting from ICSI even when the DNA Fragmentation index is reduced to 15% or even 5%
title_short	IVF-ICSI split insemination reveals those cases of unexplained infertility benefitting from ICSI even when the DNA Fragmentation index is reduced to 15% or even 5%
title_full	IVF-ICSI split insemination reveals those cases of unexplained infertility benefitting from ICSI even when the DNA Fragmentation index is reduced to 15% or even 5%
title_fullStr	IVF-ICSI split insemination reveals those cases of unexplained infertility benefitting from ICSI even when the DNA Fragmentation index is reduced to 15% or even 5%
title_full_unstemmed	IVF-ICSI split insemination reveals those cases of unexplained infertility benefitting from ICSI even when the DNA Fragmentation index is reduced to 15% or even 5%
title_sort	ivf-icsi split insemination reveals those cases of unexplained infertility benefitting from icsi even when the dna fragmentation index is reduced to 15% or even 5%
publisher	Andrology & Gynecology: Current Research
publishDate	2016
url	http://irep.iium.edu.my/50707/ http://irep.iium.edu.my/50707/ http://irep.iium.edu.my/50707/ http://irep.iium.edu.my/50707/3/50707.pdf
first_indexed	2023-09-18T21:11:42Z
last_indexed	2023-09-18T21:11:42Z
_version_	1777411295907151872

IVF-ICSI split insemination reveals those cases of unexplained infertility benefitting from ICSI even when the DNA Fragmentation index is reduced to 15% or even 5%

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