Effect of solvents extraction on the antioxidant activity of Acacia polyacantha gum

Acacia polyacantha gum (APG) is a dried exudate which obtained from the stems and branches of Acacia polyacantha trees, rich in soluble dietary fibers and organic compounds that appear to be capable of preventing many diseases. This study allowed for quantitation of the levels of total phenolic cont...

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Main Authors:	Mohammedelnour, Ahmed A., Mirghani, Mohamed Elwathig Saeed, Kabbashi, Nassereldeen Ahmed, Alam, Md. Zahangir, Abdullah, Amina, Musa, Khalid Hamid
Format:	Conference or Workshop Item
Language:	English
Published:	Kulliyyah of Engineering, International Islamic University Malaysia 2016
Subjects:	TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices
Online Access:	http://irep.iium.edu.my/50917/ http://irep.iium.edu.my/50917/ http://irep.iium.edu.my/50917/1/50917_Effect_of_solvents_extraction.pdf

id	iium-50917
recordtype	eprints
spelling	iium-509172016-11-04T07:43:15Z http://irep.iium.edu.my/50917/ Effect of solvents extraction on the antioxidant activity of Acacia polyacantha gum Mohammedelnour, Ahmed A. Mirghani, Mohamed Elwathig Saeed Kabbashi, Nassereldeen Ahmed Alam, Md. Zahangir Abdullah, Amina Musa, Khalid Hamid TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices Acacia polyacantha gum (APG) is a dried exudate which obtained from the stems and branches of Acacia polyacantha trees, rich in soluble dietary fibers and organic compounds that appear to be capable of preventing many diseases. This study allowed for quantitation of the levels of total phenolic content (TPC) and antioxidant activity using ABTS and CUPRAC assays from APG extraction using pure solvents (methanol, ethanol, acetone) and their aqueous mixtures at 50% and 100%. The antioxidant levels were evaluated by 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) radical cation decolourisation and cupric iron reducing capacity in the presence of neocuproine (CUPRAC) for the evaluation of reducing power, and (TPC) was evaluated by the Folin-Ciocalteu method. As results, the solvent methanol (50%) gave the best extraction rates for APG presented by highest TPC (60.7833 ± 0.0416), CUPRAC (34.6533± 0.0611 mg TF/ 100g) and ABTS (34.6533± 0.0611 mg TF/ 100g) followed by ethanol 50%. On the other hand, pure methanol showed the lowest TPC (05.3300 ± 0.0608 mg GAE /100g DW), ABTS (10.9000 ± 0.0436 mg TF/ 100g), and CUPRAC (7.8000± 0.1249 mg TF/ 100g) values. Therefore, the variation in the antioxidant capacity between extracts was probably due to the differences in the nature of the APG compounds extracted using different solvents. The higher content of antioxidant activity in APG may have beneficial effects on the human health. Kulliyyah of Engineering, International Islamic University Malaysia 2016 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/50917/1/50917_Effect_of_solvents_extraction.pdf Mohammedelnour, Ahmed A. and Mirghani, Mohamed Elwathig Saeed and Kabbashi, Nassereldeen Ahmed and Alam, Md. Zahangir and Abdullah, Amina and Musa, Khalid Hamid (2016) Effect of solvents extraction on the antioxidant activity of Acacia polyacantha gum. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur. http://www.iium.edu.my/icbioe/2016/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices
spellingShingle	TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices Mohammedelnour, Ahmed A. Mirghani, Mohamed Elwathig Saeed Kabbashi, Nassereldeen Ahmed Alam, Md. Zahangir Abdullah, Amina Musa, Khalid Hamid Effect of solvents extraction on the antioxidant activity of Acacia polyacantha gum
description	Acacia polyacantha gum (APG) is a dried exudate which obtained from the stems and branches of Acacia polyacantha trees, rich in soluble dietary fibers and organic compounds that appear to be capable of preventing many diseases. This study allowed for quantitation of the levels of total phenolic content (TPC) and antioxidant activity using ABTS and CUPRAC assays from APG extraction using pure solvents (methanol, ethanol, acetone) and their aqueous mixtures at 50% and 100%. The antioxidant levels were evaluated by 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) radical cation decolourisation and cupric iron reducing capacity in the presence of neocuproine (CUPRAC) for the evaluation of reducing power, and (TPC) was evaluated by the Folin-Ciocalteu method. As results, the solvent methanol (50%) gave the best extraction rates for APG presented by highest TPC (60.7833 ± 0.0416), CUPRAC (34.6533± 0.0611 mg TF/ 100g) and ABTS (34.6533± 0.0611 mg TF/ 100g) followed by ethanol 50%. On the other hand, pure methanol showed the lowest TPC (05.3300 ± 0.0608 mg GAE /100g DW), ABTS (10.9000 ± 0.0436 mg TF/ 100g), and CUPRAC (7.8000± 0.1249 mg TF/ 100g) values. Therefore, the variation in the antioxidant capacity between extracts was probably due to the differences in the nature of the APG compounds extracted using different solvents. The higher content of antioxidant activity in APG may have beneficial effects on the human health.
format	Conference or Workshop Item
author	Mohammedelnour, Ahmed A. Mirghani, Mohamed Elwathig Saeed Kabbashi, Nassereldeen Ahmed Alam, Md. Zahangir Abdullah, Amina Musa, Khalid Hamid
author_facet	Mohammedelnour, Ahmed A. Mirghani, Mohamed Elwathig Saeed Kabbashi, Nassereldeen Ahmed Alam, Md. Zahangir Abdullah, Amina Musa, Khalid Hamid
author_sort	Mohammedelnour, Ahmed A.
title	Effect of solvents extraction on the antioxidant activity of Acacia polyacantha gum
title_short	Effect of solvents extraction on the antioxidant activity of Acacia polyacantha gum
title_full	Effect of solvents extraction on the antioxidant activity of Acacia polyacantha gum
title_fullStr	Effect of solvents extraction on the antioxidant activity of Acacia polyacantha gum
title_full_unstemmed	Effect of solvents extraction on the antioxidant activity of Acacia polyacantha gum
title_sort	effect of solvents extraction on the antioxidant activity of acacia polyacantha gum
publisher	Kulliyyah of Engineering, International Islamic University Malaysia
publishDate	2016
url	http://irep.iium.edu.my/50917/ http://irep.iium.edu.my/50917/ http://irep.iium.edu.my/50917/1/50917_Effect_of_solvents_extraction.pdf
first_indexed	2023-09-18T21:12:03Z
last_indexed	2023-09-18T21:12:03Z
_version_	1777411318021619712

Effect of solvents extraction on the antioxidant activity of Acacia polyacantha gum

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