Alternative surgical approach to create osteomylitis in New Zealand White rabbit model

Introduction: The New Zealand White (NZW) rabbit is an established model for infection. Objective: An experimental protocol for local bacteria injection at distal femoral shaft to create osteomyelitis in the New Zealand White rabbit is described. Methodology: This study is a descriptive analysis of...

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Main Authors:	Zulkifly, Ahmad Hafiz, Abdul Aziz, Azian, Mohd Jan, Nurul Hafiza, Ibrahim, Mohd Zulfadzli
Format:	Article
Language:	English
Published:	Kulliyyah of Medicine, International Islamic University Malaysia 2015
Subjects:	RD701 Orthopedics
Online Access:	http://irep.iium.edu.my/51305/ http://irep.iium.edu.my/51305/ http://irep.iium.edu.my/51305/1/IMJM-Vol-14-Supp-p13-p14-Ahmad-Hafiz-Zulkifly.pdf

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recordtype	eprints
spelling	iium-513052016-07-27T08:28:48Z http://irep.iium.edu.my/51305/ Alternative surgical approach to create osteomylitis in New Zealand White rabbit model Zulkifly, Ahmad Hafiz Abdul Aziz, Azian Mohd Jan, Nurul Hafiza Ibrahim, Mohd Zulfadzli RD701 Orthopedics Introduction: The New Zealand White (NZW) rabbit is an established model for infection. Objective: An experimental protocol for local bacteria injection at distal femoral shaft to create osteomyelitis in the New Zealand White rabbit is described. Methodology: This study is a descriptive analysis of the surgical approach (and related complications) used for osteomyelitis at distal femur in the NZW model. This study was performed at Advanced Orthopaedic Research Laboratory, International Islamic University Malaysia that followed the requirements of ISO17025 standard. The procedure was to introduce sterile distilled water to wash the femoral shaft before inoculation with ATCC 25923 Staphylococcus aureus to produce osteomyelitis in the NZW rabbit. The outcomes then were analyzed through radiographic assessment and microbiology identification tests. Results: A total of 36 NZW rabbits underwent a distal femoral shaft osteomyelitis surgery. After experimental protocol established, osteomyelitis was noted and was successful infected with Staphylococcus aureus to promote osteomyelitis using the method at distal femoral shaft in all 36 NZW rabbits. We achieved 100% of inoculation of osteomyelitis in all 36 New Zealand White rabbits. No mortality noted among all rabbits involved in this study. Conclusion: This is a simple and reliable method was demonstrated and recommended for surgery via femoral shaft on New Zealand White rabbits to create osteomyelitis. Kulliyyah of Medicine, International Islamic University Malaysia 2015-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/51305/1/IMJM-Vol-14-Supp-p13-p14-Ahmad-Hafiz-Zulkifly.pdf Zulkifly, Ahmad Hafiz and Abdul Aziz, Azian and Mohd Jan, Nurul Hafiza and Ibrahim, Mohd Zulfadzli (2015) Alternative surgical approach to create osteomylitis in New Zealand White rabbit model. The International Medical Journal Malaysia, 14 (supp. issue). pp. 13-14. ISSN 1823-4631 http://iiumedic.net/imjm/v1/download/volume_14_supplement/IMJM-Vol-14-Supp-p13-p14-Ahmad-Hafiz-Zulkifly.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RD701 Orthopedics
spellingShingle	RD701 Orthopedics Zulkifly, Ahmad Hafiz Abdul Aziz, Azian Mohd Jan, Nurul Hafiza Ibrahim, Mohd Zulfadzli Alternative surgical approach to create osteomylitis in New Zealand White rabbit model
description	Introduction: The New Zealand White (NZW) rabbit is an established model for infection. Objective: An experimental protocol for local bacteria injection at distal femoral shaft to create osteomyelitis in the New Zealand White rabbit is described. Methodology: This study is a descriptive analysis of the surgical approach (and related complications) used for osteomyelitis at distal femur in the NZW model. This study was performed at Advanced Orthopaedic Research Laboratory, International Islamic University Malaysia that followed the requirements of ISO17025 standard. The procedure was to introduce sterile distilled water to wash the femoral shaft before inoculation with ATCC 25923 Staphylococcus aureus to produce osteomyelitis in the NZW rabbit. The outcomes then were analyzed through radiographic assessment and microbiology identification tests. Results: A total of 36 NZW rabbits underwent a distal femoral shaft osteomyelitis surgery. After experimental protocol established, osteomyelitis was noted and was successful infected with Staphylococcus aureus to promote osteomyelitis using the method at distal femoral shaft in all 36 NZW rabbits. We achieved 100% of inoculation of osteomyelitis in all 36 New Zealand White rabbits. No mortality noted among all rabbits involved in this study. Conclusion: This is a simple and reliable method was demonstrated and recommended for surgery via femoral shaft on New Zealand White rabbits to create osteomyelitis.
format	Article
author	Zulkifly, Ahmad Hafiz Abdul Aziz, Azian Mohd Jan, Nurul Hafiza Ibrahim, Mohd Zulfadzli
author_facet	Zulkifly, Ahmad Hafiz Abdul Aziz, Azian Mohd Jan, Nurul Hafiza Ibrahim, Mohd Zulfadzli
author_sort	Zulkifly, Ahmad Hafiz
title	Alternative surgical approach to create osteomylitis in New Zealand White rabbit model
title_short	Alternative surgical approach to create osteomylitis in New Zealand White rabbit model
title_full	Alternative surgical approach to create osteomylitis in New Zealand White rabbit model
title_fullStr	Alternative surgical approach to create osteomylitis in New Zealand White rabbit model
title_full_unstemmed	Alternative surgical approach to create osteomylitis in New Zealand White rabbit model
title_sort	alternative surgical approach to create osteomylitis in new zealand white rabbit model
publisher	Kulliyyah of Medicine, International Islamic University Malaysia
publishDate	2015
url	http://irep.iium.edu.my/51305/ http://irep.iium.edu.my/51305/ http://irep.iium.edu.my/51305/1/IMJM-Vol-14-Supp-p13-p14-Ahmad-Hafiz-Zulkifly.pdf
first_indexed	2023-09-18T21:12:37Z
last_indexed	2023-09-18T21:12:37Z
_version_	1777411353334513664

Alternative surgical approach to create osteomylitis in New Zealand White rabbit model

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