Quantification of anti-fertility compound-Diosgenin concentration in the fenugreek seeds aqueous extrac (FSA)

This study aims to build a standardization method for preparation of effective powder from FSA and to quantify diosgenin in FSA. Methodology: One kg of FS were used in this study. Setting: BMS, KOM and KOP, IIUM Kuantan campus. FS were washed with distilled water to exclude any foreign matter, and w...

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Main Authors:	Allow, Ahmed Kaid Naji, Mohamed Bakrim, Norbaiyah, Al-Ani, Imad Matloub Dally, Mohd Hassan, Norazian
Format:	Article
Language:	English English
Published:	IIUM Press 2016
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/51310/ http://irep.iium.edu.my/51310/ http://irep.iium.edu.my/51310/1/51310_Quantification_of_anti-fertility.pdf http://irep.iium.edu.my/51310/4/51310_Quantification%20of%20anti-fertility%20compound-Diosgenin%20concentration_Scopus.pdf

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spelling	iium-513102017-04-05T01:19:19Z http://irep.iium.edu.my/51310/ Quantification of anti-fertility compound-Diosgenin concentration in the fenugreek seeds aqueous extrac (FSA) Allow, Ahmed Kaid Naji Mohamed Bakrim, Norbaiyah Al-Ani, Imad Matloub Dally Mohd Hassan, Norazian R Medicine (General) This study aims to build a standardization method for preparation of effective powder from FSA and to quantify diosgenin in FSA. Methodology: One kg of FS were used in this study. Setting: BMS, KOM and KOP, IIUM Kuantan campus. FS were washed with distilled water to exclude any foreign matter, and were then air dried. FS-powder were put in distilled water in a ratio of 1 g of powder in 20 ml of distilled water and were shaken at room temperature for 24 hours. Ten mg of hydrolyzed extract sample was diluted in 10 ml volumetric flask with methanol for 15 minutes. Chromatographic estimation was performed using an equilibrated reverse phase Eclipse XDB-C18 column (particle size 5 μg, 4.6 mm x 150 mm). Results: One gram of FSA extract was hydrolyzed to produce sapogenins and 46.6% was recovered. A calibration curve that was constructed based on five dilutions of diosgenin standard at concentrations of 2, 5, 10, 20, 30 and 50 ppm produced a linear graft (r = 0.999). The concentration of diosgenin in FSA extract as calculated using the regression analysis was found to be 29.66 μg/ml, 13.81 % w/w on dried weight basis. Conclusion: Preparation and standardization of effective powder from FSA are the corner stone of many scientific researches in IIUM and Malaysia. Diosgenin is available in the FSA in adequate concentration. The adequate amount of diosgenin in the FSA will guide us to do further study in the way of preparation of a natural product that can be used in the field of reversible anti-fertility therapy. IIUM Press 2016-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/51310/1/51310_Quantification_of_anti-fertility.pdf application/pdf en http://irep.iium.edu.my/51310/4/51310_Quantification%20of%20anti-fertility%20compound-Diosgenin%20concentration_Scopus.pdf Allow, Ahmed Kaid Naji and Mohamed Bakrim, Norbaiyah and Al-Ani, Imad Matloub Dally and Mohd Hassan, Norazian (2016) Quantification of anti-fertility compound-Diosgenin concentration in the fenugreek seeds aqueous extrac (FSA). The International Medical Journal Malaysia, 15 (1). pp. 75-80. ISSN 1823-4631 http://journals.iium.edu.my/imjm/index.php/eimj/article/view/425
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Allow, Ahmed Kaid Naji Mohamed Bakrim, Norbaiyah Al-Ani, Imad Matloub Dally Mohd Hassan, Norazian Quantification of anti-fertility compound-Diosgenin concentration in the fenugreek seeds aqueous extrac (FSA)
description	This study aims to build a standardization method for preparation of effective powder from FSA and to quantify diosgenin in FSA. Methodology: One kg of FS were used in this study. Setting: BMS, KOM and KOP, IIUM Kuantan campus. FS were washed with distilled water to exclude any foreign matter, and were then air dried. FS-powder were put in distilled water in a ratio of 1 g of powder in 20 ml of distilled water and were shaken at room temperature for 24 hours. Ten mg of hydrolyzed extract sample was diluted in 10 ml volumetric flask with methanol for 15 minutes. Chromatographic estimation was performed using an equilibrated reverse phase Eclipse XDB-C18 column (particle size 5 μg, 4.6 mm x 150 mm). Results: One gram of FSA extract was hydrolyzed to produce sapogenins and 46.6% was recovered. A calibration curve that was constructed based on five dilutions of diosgenin standard at concentrations of 2, 5, 10, 20, 30 and 50 ppm produced a linear graft (r = 0.999). The concentration of diosgenin in FSA extract as calculated using the regression analysis was found to be 29.66 μg/ml, 13.81 % w/w on dried weight basis. Conclusion: Preparation and standardization of effective powder from FSA are the corner stone of many scientific researches in IIUM and Malaysia. Diosgenin is available in the FSA in adequate concentration. The adequate amount of diosgenin in the FSA will guide us to do further study in the way of preparation of a natural product that can be used in the field of reversible anti-fertility therapy.
format	Article
author	Allow, Ahmed Kaid Naji Mohamed Bakrim, Norbaiyah Al-Ani, Imad Matloub Dally Mohd Hassan, Norazian
author_facet	Allow, Ahmed Kaid Naji Mohamed Bakrim, Norbaiyah Al-Ani, Imad Matloub Dally Mohd Hassan, Norazian
author_sort	Allow, Ahmed Kaid Naji
title	Quantification of anti-fertility compound-Diosgenin concentration in the fenugreek seeds aqueous extrac (FSA)
title_short	Quantification of anti-fertility compound-Diosgenin concentration in the fenugreek seeds aqueous extrac (FSA)
title_full	Quantification of anti-fertility compound-Diosgenin concentration in the fenugreek seeds aqueous extrac (FSA)
title_fullStr	Quantification of anti-fertility compound-Diosgenin concentration in the fenugreek seeds aqueous extrac (FSA)
title_full_unstemmed	Quantification of anti-fertility compound-Diosgenin concentration in the fenugreek seeds aqueous extrac (FSA)
title_sort	quantification of anti-fertility compound-diosgenin concentration in the fenugreek seeds aqueous extrac (fsa)
publisher	IIUM Press
publishDate	2016
url	http://irep.iium.edu.my/51310/ http://irep.iium.edu.my/51310/ http://irep.iium.edu.my/51310/1/51310_Quantification_of_anti-fertility.pdf http://irep.iium.edu.my/51310/4/51310_Quantification%20of%20anti-fertility%20compound-Diosgenin%20concentration_Scopus.pdf
first_indexed	2023-09-18T21:12:37Z
last_indexed	2023-09-18T21:12:37Z
_version_	1777411353942687744

Quantification of anti-fertility compound-Diosgenin concentration in the fenugreek seeds aqueous extrac (FSA)

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