Effect of perivitelline fluid from horseshoe crab on the expression of cell cycle regulatory genes in human dental pulp stem cells

Abstract Perivitelline fluid (PVF) of the horseshoe crab embryo has been reported to possess an important role during embryogenesis by promoting cell proliferation. This study aims to evaluate the effect of PVF on the expression of cell cycle regulatory genes from human dental pulp stem cells (DPSCs...

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Main Authors:	Rani, Abdul Qawee, kannan, Thirumulu Ponnuraj, Azmi, Nur Izyan, Ibrahim, Najian, Omar, Nor Shamsuria, Ahmad, Azlina, Mokhtar, Khairani Idah
Format:	Article
Language:	English English
Published:	Universiti Sains Malaysia 2016
Subjects:	RK Dentistry
Online Access:	http://irep.iium.edu.my/51322/ http://irep.iium.edu.my/51322/ http://irep.iium.edu.my/51322/1/2016_AOS-Effect_of_perivitelline_fluid_from_horseshoe_crab_on_the_expression_of_cell_cycle_regulatory_gene_on_human_dental_pulp_cells.pdf http://irep.iium.edu.my/51322/4/Effect%20of%20perivitelline%20fluid%20from%20horseshoe%20crab%20on%20the%20expression%20of%20cell%20cycle%20regulatory%20genes%20in%20human%20dental%20pulp%20stem%20cells.pdf

id	iium-51322
recordtype	eprints
spelling	iium-513222018-01-12T02:11:51Z http://irep.iium.edu.my/51322/ Effect of perivitelline fluid from horseshoe crab on the expression of cell cycle regulatory genes in human dental pulp stem cells Rani, Abdul Qawee kannan, Thirumulu Ponnuraj Azmi, Nur Izyan Ibrahim, Najian Omar, Nor Shamsuria Ahmad, Azlina Mokhtar, Khairani Idah RK Dentistry Abstract Perivitelline fluid (PVF) of the horseshoe crab embryo has been reported to possess an important role during embryogenesis by promoting cell proliferation. This study aims to evaluate the effect of PVF on the expression of cell cycle regulatory genes from human dental pulp stem cells (DPSCs) between different cell passages viz. 4, 5, 6. The cells were treated with a single dose of PVF (26.89 mg/ml) PVF. Gene expression was quantified for CDKNA2A, PTEN, MDM2 and TP53 genes using reverse transcriptase PCR. CDKN2A and MDM2 expression for treated and untreated DPSCs, expressed a similar pattern of expression. The higher expression of CDKN2A showed that the treatment increased cell proliferation and prevented cell senescence. DPSCs with PVF treatment showed increased expression of MDM2 at passage 4 and drastically declined expression at passage 5 and slightly increased at passage 6. TP53 expression of DPSCs treated group showed a higher expression compared to untreated group. On the other hand, the expression of PTEN in DPSCs treated group started to increase from passage 5 to 6. However, on the whole, the PTEN expression was higher than the untreated group in all the passages studied here. The results showed that PVF could enhance cell cycle regulatory gene expression in DPSCs as indicated by the higher expression of all the genes considered in this study at different cell passages in the treated group compared to the untreated group. Mann Whitney test was utilized to determine the significance of cell cycle regulatory genes expression between treated and untreated group. Significant difference in expression of genes between the treated and untreated groups were found at all passages except for CDKN2A gene whereby, its expression was not significantly different at passage 5 though it did express slightly higher in PVF treated DPSCs. Keywords: cell cycle, dental pulp stem cells, gene expression, horseshoe crab, perivitelline fluid. Universiti Sains Malaysia 2016 Article PeerReviewed application/pdf en http://irep.iium.edu.my/51322/1/2016_AOS-Effect_of_perivitelline_fluid_from_horseshoe_crab_on_the_expression_of_cell_cycle_regulatory_gene_on_human_dental_pulp_cells.pdf application/pdf en http://irep.iium.edu.my/51322/4/Effect%20of%20perivitelline%20fluid%20from%20horseshoe%20crab%20on%20the%20expression%20of%20cell%20cycle%20regulatory%20genes%20in%20human%20dental%20pulp%20stem%20cells.pdf Rani, Abdul Qawee and kannan, Thirumulu Ponnuraj and Azmi, Nur Izyan and Ibrahim, Najian and Omar, Nor Shamsuria and Ahmad, Azlina and Mokhtar, Khairani Idah (2016) Effect of perivitelline fluid from horseshoe crab on the expression of cell cycle regulatory genes in human dental pulp stem cells. Archives of Orofacial Sciences, 11 (1). pp. 7-14. ISSN 1823-8602 E-ISSN 2231-7163 http://www.dental.usm.my/aos/docs/Vol_11/aos-article-0226.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RK Dentistry
spellingShingle	RK Dentistry Rani, Abdul Qawee kannan, Thirumulu Ponnuraj Azmi, Nur Izyan Ibrahim, Najian Omar, Nor Shamsuria Ahmad, Azlina Mokhtar, Khairani Idah Effect of perivitelline fluid from horseshoe crab on the expression of cell cycle regulatory genes in human dental pulp stem cells
description	Abstract Perivitelline fluid (PVF) of the horseshoe crab embryo has been reported to possess an important role during embryogenesis by promoting cell proliferation. This study aims to evaluate the effect of PVF on the expression of cell cycle regulatory genes from human dental pulp stem cells (DPSCs) between different cell passages viz. 4, 5, 6. The cells were treated with a single dose of PVF (26.89 mg/ml) PVF. Gene expression was quantified for CDKNA2A, PTEN, MDM2 and TP53 genes using reverse transcriptase PCR. CDKN2A and MDM2 expression for treated and untreated DPSCs, expressed a similar pattern of expression. The higher expression of CDKN2A showed that the treatment increased cell proliferation and prevented cell senescence. DPSCs with PVF treatment showed increased expression of MDM2 at passage 4 and drastically declined expression at passage 5 and slightly increased at passage 6. TP53 expression of DPSCs treated group showed a higher expression compared to untreated group. On the other hand, the expression of PTEN in DPSCs treated group started to increase from passage 5 to 6. However, on the whole, the PTEN expression was higher than the untreated group in all the passages studied here. The results showed that PVF could enhance cell cycle regulatory gene expression in DPSCs as indicated by the higher expression of all the genes considered in this study at different cell passages in the treated group compared to the untreated group. Mann Whitney test was utilized to determine the significance of cell cycle regulatory genes expression between treated and untreated group. Significant difference in expression of genes between the treated and untreated groups were found at all passages except for CDKN2A gene whereby, its expression was not significantly different at passage 5 though it did express slightly higher in PVF treated DPSCs. Keywords: cell cycle, dental pulp stem cells, gene expression, horseshoe crab, perivitelline fluid.
format	Article
author	Rani, Abdul Qawee kannan, Thirumulu Ponnuraj Azmi, Nur Izyan Ibrahim, Najian Omar, Nor Shamsuria Ahmad, Azlina Mokhtar, Khairani Idah
author_facet	Rani, Abdul Qawee kannan, Thirumulu Ponnuraj Azmi, Nur Izyan Ibrahim, Najian Omar, Nor Shamsuria Ahmad, Azlina Mokhtar, Khairani Idah
author_sort	Rani, Abdul Qawee
title	Effect of perivitelline fluid from horseshoe crab on the expression of cell cycle regulatory genes in human dental pulp stem cells
title_short	Effect of perivitelline fluid from horseshoe crab on the expression of cell cycle regulatory genes in human dental pulp stem cells
title_full	Effect of perivitelline fluid from horseshoe crab on the expression of cell cycle regulatory genes in human dental pulp stem cells
title_fullStr	Effect of perivitelline fluid from horseshoe crab on the expression of cell cycle regulatory genes in human dental pulp stem cells
title_full_unstemmed	Effect of perivitelline fluid from horseshoe crab on the expression of cell cycle regulatory genes in human dental pulp stem cells
title_sort	effect of perivitelline fluid from horseshoe crab on the expression of cell cycle regulatory genes in human dental pulp stem cells
publisher	Universiti Sains Malaysia
publishDate	2016
url	http://irep.iium.edu.my/51322/ http://irep.iium.edu.my/51322/ http://irep.iium.edu.my/51322/1/2016_AOS-Effect_of_perivitelline_fluid_from_horseshoe_crab_on_the_expression_of_cell_cycle_regulatory_gene_on_human_dental_pulp_cells.pdf http://irep.iium.edu.my/51322/4/Effect%20of%20perivitelline%20fluid%20from%20horseshoe%20crab%20on%20the%20expression%20of%20cell%20cycle%20regulatory%20genes%20in%20human%20dental%20pulp%20stem%20cells.pdf
first_indexed	2023-09-18T21:12:39Z
last_indexed	2023-09-18T21:12:39Z
_version_	1777411355091927040

Effect of perivitelline fluid from horseshoe crab on the expression of cell cycle regulatory genes in human dental pulp stem cells

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