Cytotoxic, anti-inflammatory and adipogenic effects of inophyllum D, calanone,isocordato-oblongic acid, and morelloflavone on cell lines

This paper reports in vitro cytotoxic, anti-inflammatory and adipocyte diffentiation with adipogenic effects of coumarins inophyllum D (1) and calanone (2), and a chromanone carboxylic acid namely isocordatooblongic acid (3) isolated from Calophyllum symingtonianum as well as a biflavonoid morellofl...

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Main Authors:	Bakhtiar, M. Taher, Aminuddin, Amnani, Darnis, Deny Susanti, Aminudin, Nurul Iman, On, Shamsul, Ahmad, Farediah, Hamidon, Hanisuhana
Format:	Article
Language:	English English
Published:	The Korean Society of Pharmacognosy 2016
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/51411/ http://irep.iium.edu.my/51411/ http://irep.iium.edu.my/51411/ http://irep.iium.edu.my/51411/1/nps-22-122.pdf http://irep.iium.edu.my/51411/4/51411_Cytotoxic%2C%20anti-inflammatory_scopus.pdf

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recordtype	eprints
spelling	iium-514112017-04-10T07:57:29Z http://irep.iium.edu.my/51411/ Cytotoxic, anti-inflammatory and adipogenic effects of inophyllum D, calanone,isocordato-oblongic acid, and morelloflavone on cell lines Bakhtiar, M. Taher Aminuddin, Amnani Darnis, Deny Susanti Aminudin, Nurul Iman On, Shamsul Ahmad, Farediah Hamidon, Hanisuhana RS Pharmacy and materia medica This paper reports in vitro cytotoxic, anti-inflammatory and adipocyte diffentiation with adipogenic effects of coumarins inophyllum D (1) and calanone (2), and a chromanone carboxylic acid namely isocordatooblongic acid (3) isolated from Calophyllum symingtonianum as well as a biflavonoid morelloflavone (4) isolated from Garcinia prainiana on MCF-7 breast adenocarcinoma RAW 264.7 macrophages and 3T3-L1 preadipocytes cells, respectively. The cytotoxicity study on MCF-7 cell was conducted by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay. Meanwhile, the study of anti-inflammatory effects in RAW 264.7 macrophages and adipogenic effects on 3T3-L1 pre-adipocytes were conducted through nitrite determination assay and induction of adipocyte differentiation, respectively. In the cytotoxicity study, inophyllum D (1) was the only compounds that exhibited significant cytotoxic effect against MCF-7 cell with IC50 of 84 μg/mL. Further, all by inhibiting the compounds have shown anti-inflammatory effects in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages of nitrite concentration with production. In addition, the compounds also exhibited adipogenic effects on 3T3-L1 pre-adipocytes by stimulating lipid formation. Thus, this study may provide significant input in discovery of the potential effects cytotoxic, anti-inflammatory and adipogenic agents. The Korean Society of Pharmacognosy 2016 Article PeerReviewed application/pdf en http://irep.iium.edu.my/51411/1/nps-22-122.pdf application/pdf en http://irep.iium.edu.my/51411/4/51411_Cytotoxic%2C%20anti-inflammatory_scopus.pdf Bakhtiar, M. Taher and Aminuddin, Amnani and Darnis, Deny Susanti and Aminudin, Nurul Iman and On, Shamsul and Ahmad, Farediah and Hamidon, Hanisuhana (2016) Cytotoxic, anti-inflammatory and adipogenic effects of inophyllum D, calanone,isocordato-oblongic acid, and morelloflavone on cell lines. Natural Products Sciences, 22 (2). pp. 122-128. ISSN 1226-3907 E-ISSN 2288-9027 http://www.e-nps.or.kr/ 10.20307/nps.2016.22.2.122
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Bakhtiar, M. Taher Aminuddin, Amnani Darnis, Deny Susanti Aminudin, Nurul Iman On, Shamsul Ahmad, Farediah Hamidon, Hanisuhana Cytotoxic, anti-inflammatory and adipogenic effects of inophyllum D, calanone,isocordato-oblongic acid, and morelloflavone on cell lines
description	This paper reports in vitro cytotoxic, anti-inflammatory and adipocyte diffentiation with adipogenic effects of coumarins inophyllum D (1) and calanone (2), and a chromanone carboxylic acid namely isocordatooblongic acid (3) isolated from Calophyllum symingtonianum as well as a biflavonoid morelloflavone (4) isolated from Garcinia prainiana on MCF-7 breast adenocarcinoma RAW 264.7 macrophages and 3T3-L1 preadipocytes cells, respectively. The cytotoxicity study on MCF-7 cell was conducted by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay. Meanwhile, the study of anti-inflammatory effects in RAW 264.7 macrophages and adipogenic effects on 3T3-L1 pre-adipocytes were conducted through nitrite determination assay and induction of adipocyte differentiation, respectively. In the cytotoxicity study, inophyllum D (1) was the only compounds that exhibited significant cytotoxic effect against MCF-7 cell with IC50 of 84 μg/mL. Further, all by inhibiting the compounds have shown anti-inflammatory effects in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages of nitrite concentration with production. In addition, the compounds also exhibited adipogenic effects on 3T3-L1 pre-adipocytes by stimulating lipid formation. Thus, this study may provide significant input in discovery of the potential effects cytotoxic, anti-inflammatory and adipogenic agents.
format	Article
author	Bakhtiar, M. Taher Aminuddin, Amnani Darnis, Deny Susanti Aminudin, Nurul Iman On, Shamsul Ahmad, Farediah Hamidon, Hanisuhana
author_facet	Bakhtiar, M. Taher Aminuddin, Amnani Darnis, Deny Susanti Aminudin, Nurul Iman On, Shamsul Ahmad, Farediah Hamidon, Hanisuhana
author_sort	Bakhtiar, M. Taher
title	Cytotoxic, anti-inflammatory and adipogenic effects of inophyllum D, calanone,isocordato-oblongic acid, and morelloflavone on cell lines
title_short	Cytotoxic, anti-inflammatory and adipogenic effects of inophyllum D, calanone,isocordato-oblongic acid, and morelloflavone on cell lines
title_full	Cytotoxic, anti-inflammatory and adipogenic effects of inophyllum D, calanone,isocordato-oblongic acid, and morelloflavone on cell lines
title_fullStr	Cytotoxic, anti-inflammatory and adipogenic effects of inophyllum D, calanone,isocordato-oblongic acid, and morelloflavone on cell lines
title_full_unstemmed	Cytotoxic, anti-inflammatory and adipogenic effects of inophyllum D, calanone,isocordato-oblongic acid, and morelloflavone on cell lines
title_sort	cytotoxic, anti-inflammatory and adipogenic effects of inophyllum d, calanone,isocordato-oblongic acid, and morelloflavone on cell lines
publisher	The Korean Society of Pharmacognosy
publishDate	2016
url	http://irep.iium.edu.my/51411/ http://irep.iium.edu.my/51411/ http://irep.iium.edu.my/51411/ http://irep.iium.edu.my/51411/1/nps-22-122.pdf http://irep.iium.edu.my/51411/4/51411_Cytotoxic%2C%20anti-inflammatory_scopus.pdf
first_indexed	2023-09-18T21:12:47Z
last_indexed	2023-09-18T21:12:47Z
_version_	1777411363852779520

Cytotoxic, anti-inflammatory and adipogenic effects of inophyllum D, calanone,isocordato-oblongic acid, and morelloflavone on cell lines

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