Formulation development and systematic optimization of production fish gelatine nanoparticles as new resource for drug delivery

Animal proteins have been chosen as promising material for producing nanoparticle and its applications have become popular on the drug delivery research area. Furthermore, gelatine nanoparticles is best biodegradable material, biocompatible, and chemical modification potential, due to its characteri...

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Main Authors:	Subara, Deni, Jaswir, Irwandi, Noorbatcha, Ibrahim Ali, Al-Khatib, Ma An Fahmi Rashid
Format:	Conference or Workshop Item
Language:	English
Published:	Kulliyyah of Engineering, International Islamic University Malaysia 2016
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/51515/ http://irep.iium.edu.my/51515/ http://irep.iium.edu.my/51515/1/51515_Formulation_Development_and_Systematic_Optimization.pdf

id	iium-51515
recordtype	eprints
spelling	iium-515152017-01-13T07:53:50Z http://irep.iium.edu.my/51515/ Formulation development and systematic optimization of production fish gelatine nanoparticles as new resource for drug delivery Subara, Deni Jaswir, Irwandi Noorbatcha, Ibrahim Ali Al-Khatib, Ma An Fahmi Rashid TP248.13 Biotechnology Animal proteins have been chosen as promising material for producing nanoparticle and its applications have become popular on the drug delivery research area. Furthermore, gelatine nanoparticles is best biodegradable material, biocompatible, and chemical modification potential, due to its characteristic, gelatine nanoparticles are very promising in carrier system for drug delivery. Mostly the commercial gelatines are derived from mammalian sources, mainly porcine and bovine. Recently, fish gelatine has provided alternative resources for religious, safety and economic reason. In this present research, the fish gelatine was used as a material for production gelatine nanoparticles. The long-term goal of this study is to develop a nanoparticulate formulation based on halal gelatine, for delivery of fragile molecules such as protein and peptide drug. Fish gelatine nanoparticles were synthesized via a two-step desolvation method. Furthermore, the aim of the present study was to assess various formulation parameters in the first step from two step desolvation method to produce fish gelatine nanoparticles that have minimum in size. In the first step, high molecular weight (HMW) fraction content was optimized by SDS-PAGE. The nanoparticles formed were further characterized for mean size, size distribution using a Zetasizer nano, while the morphology of the particles was evaluated by field emission scanning electron microscope (FESEM). The smallest size of achieved nanoparticles was 227 nm which is suitable for drug delivery. The study indicated that a high fraction of HMW in first step could be obtained with gelatine concentration 9%, temperature 45 °C, centrifugation speed at 9700 rpm, and centrifugation time was 5 minute. The mechanistic of the optimum conditions for producing fish gelatine nanoparticles as well as their characterization are discussed. Kulliyyah of Engineering, International Islamic University Malaysia 2016 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/51515/1/51515_Formulation_Development_and_Systematic_Optimization.pdf Subara, Deni and Jaswir, Irwandi and Noorbatcha, Ibrahim Ali and Al-Khatib, Ma An Fahmi Rashid (2016) Formulation development and systematic optimization of production fish gelatine nanoparticles as new resource for drug delivery. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur. http://www.iium.edu.my/icbioe/2016/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Subara, Deni Jaswir, Irwandi Noorbatcha, Ibrahim Ali Al-Khatib, Ma An Fahmi Rashid Formulation development and systematic optimization of production fish gelatine nanoparticles as new resource for drug delivery
description	Animal proteins have been chosen as promising material for producing nanoparticle and its applications have become popular on the drug delivery research area. Furthermore, gelatine nanoparticles is best biodegradable material, biocompatible, and chemical modification potential, due to its characteristic, gelatine nanoparticles are very promising in carrier system for drug delivery. Mostly the commercial gelatines are derived from mammalian sources, mainly porcine and bovine. Recently, fish gelatine has provided alternative resources for religious, safety and economic reason. In this present research, the fish gelatine was used as a material for production gelatine nanoparticles. The long-term goal of this study is to develop a nanoparticulate formulation based on halal gelatine, for delivery of fragile molecules such as protein and peptide drug. Fish gelatine nanoparticles were synthesized via a two-step desolvation method. Furthermore, the aim of the present study was to assess various formulation parameters in the first step from two step desolvation method to produce fish gelatine nanoparticles that have minimum in size. In the first step, high molecular weight (HMW) fraction content was optimized by SDS-PAGE. The nanoparticles formed were further characterized for mean size, size distribution using a Zetasizer nano, while the morphology of the particles was evaluated by field emission scanning electron microscope (FESEM). The smallest size of achieved nanoparticles was 227 nm which is suitable for drug delivery. The study indicated that a high fraction of HMW in first step could be obtained with gelatine concentration 9%, temperature 45 °C, centrifugation speed at 9700 rpm, and centrifugation time was 5 minute. The mechanistic of the optimum conditions for producing fish gelatine nanoparticles as well as their characterization are discussed.
format	Conference or Workshop Item
author	Subara, Deni Jaswir, Irwandi Noorbatcha, Ibrahim Ali Al-Khatib, Ma An Fahmi Rashid
author_facet	Subara, Deni Jaswir, Irwandi Noorbatcha, Ibrahim Ali Al-Khatib, Ma An Fahmi Rashid
author_sort	Subara, Deni
title	Formulation development and systematic optimization of production fish gelatine nanoparticles as new resource for drug delivery
title_short	Formulation development and systematic optimization of production fish gelatine nanoparticles as new resource for drug delivery
title_full	Formulation development and systematic optimization of production fish gelatine nanoparticles as new resource for drug delivery
title_fullStr	Formulation development and systematic optimization of production fish gelatine nanoparticles as new resource for drug delivery
title_full_unstemmed	Formulation development and systematic optimization of production fish gelatine nanoparticles as new resource for drug delivery
title_sort	formulation development and systematic optimization of production fish gelatine nanoparticles as new resource for drug delivery
publisher	Kulliyyah of Engineering, International Islamic University Malaysia
publishDate	2016
url	http://irep.iium.edu.my/51515/ http://irep.iium.edu.my/51515/ http://irep.iium.edu.my/51515/1/51515_Formulation_Development_and_Systematic_Optimization.pdf
first_indexed	2023-09-18T21:12:56Z
last_indexed	2023-09-18T21:12:56Z
_version_	1777411373183008768

Formulation development and systematic optimization of production fish gelatine nanoparticles as new resource for drug delivery

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