Formation of human articular cartilage from in vitro to in vivo short-term ectopic implantation model: Gross observation, histology and immunohistochemistry analysis

This study aimed to explore the formation of hyaline-like articular cartilage substitute using primary cells derived from osteoarthritic patients overexpressed with SRY(Sex Determining Region Y)-Box9 (SOX9)geneseeded on poly(lactic-co-glycolic acid) (PLGA) with or without fibrin as cells carrier t...

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Main Authors:	Sha’ban, Munirah, Mohamad Sukri, Norhamiza, Ahmad Radzi, Muhammad Aa’zamuddin, Abdul Rahman, Rozlin, Mohamad, Mohd Yusof, Md Nazir, Noorhidayah, Zulkifly, Ahmad Hafiz, Hashi, Abdurezak Abdulahi
Format:	Conference or Workshop Item
Language:	English
Published:	Kulliyah of Engineering, International Islamic University Malaysia 2016
Subjects:	RD701 Orthopedics TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/51700/ http://irep.iium.edu.my/51700/ http://irep.iium.edu.my/51700/1/51700.pdf

id	iium-51700
recordtype	eprints
spelling	iium-517002016-08-19T03:22:04Z http://irep.iium.edu.my/51700/ Formation of human articular cartilage from in vitro to in vivo short-term ectopic implantation model: Gross observation, histology and immunohistochemistry analysis Sha’ban, Munirah Mohamad Sukri, Norhamiza Ahmad Radzi, Muhammad Aa’zamuddin Abdul Rahman, Rozlin Mohamad, Mohd Yusof Md Nazir, Noorhidayah Zulkifly, Ahmad Hafiz Hashi, Abdurezak Abdulahi RD701 Orthopedics TP248.13 Biotechnology This study aimed to explore the formation of hyaline-like articular cartilage substitute using primary cells derived from osteoarthritic patients overexpressed with SRY(Sex Determining Region Y)-Box9 (SOX9)geneseeded on poly(lactic-co-glycolic acid) (PLGA) with or without fibrin as cells carrier through in-vitro and in vivo experiment settings using the ectopic implantation model i.e. athymic mice. Based on the approved research protocol, the TECs were formed and cultured for three weeks in vitro. The TECs were then implanted subcutaneously at the dorsum of athymic mice for four weeks. All TECs were subjected to gross inspection, histology, and immunohistochemistry (IHC) evaluation. Macroscopically, all in vitro TECs exhibited smooth, white and glossy hyaline-like cartilage appearance. After implantation, the 2-week TECs showed a favourable hyaline-like cartilage appearance when compared to the 4-week TECs. Histologically, all in vitro TECs exhibited minimally or lack cartilaginous tissue formation. The presence of high density, lacunae-encapsulated chondrocytes, distributed in cartilaginous matrices was observed in most postimplanted TECs. Coupled with positive immunoreaction against collagen type II (the cartilage-specific marker), cartilaginous properties were noticeable more in the 2-week than the 4-week post-implanted TECs. Kulliyah of Engineering, International Islamic University Malaysia 2016 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/51700/1/51700.pdf Sha’ban, Munirah and Mohamad Sukri, Norhamiza and Ahmad Radzi, Muhammad Aa’zamuddin and Abdul Rahman, Rozlin and Mohamad, Mohd Yusof and Md Nazir, Noorhidayah and Zulkifly, Ahmad Hafiz and Hashi, Abdurezak Abdulahi (2016) Formation of human articular cartilage from in vitro to in vivo short-term ectopic implantation model: Gross observation, histology and immunohistochemistry analysis. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur. http://www.iium.edu.my/icbioe/2016/index.php/78-the-4th-international-conference-on-biotechnology-engineering
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RD701 Orthopedics TP248.13 Biotechnology
spellingShingle	RD701 Orthopedics TP248.13 Biotechnology Sha’ban, Munirah Mohamad Sukri, Norhamiza Ahmad Radzi, Muhammad Aa’zamuddin Abdul Rahman, Rozlin Mohamad, Mohd Yusof Md Nazir, Noorhidayah Zulkifly, Ahmad Hafiz Hashi, Abdurezak Abdulahi Formation of human articular cartilage from in vitro to in vivo short-term ectopic implantation model: Gross observation, histology and immunohistochemistry analysis
description	This study aimed to explore the formation of hyaline-like articular cartilage substitute using primary cells derived from osteoarthritic patients overexpressed with SRY(Sex Determining Region Y)-Box9 (SOX9)geneseeded on poly(lactic-co-glycolic acid) (PLGA) with or without fibrin as cells carrier through in-vitro and in vivo experiment settings using the ectopic implantation model i.e. athymic mice. Based on the approved research protocol, the TECs were formed and cultured for three weeks in vitro. The TECs were then implanted subcutaneously at the dorsum of athymic mice for four weeks. All TECs were subjected to gross inspection, histology, and immunohistochemistry (IHC) evaluation. Macroscopically, all in vitro TECs exhibited smooth, white and glossy hyaline-like cartilage appearance. After implantation, the 2-week TECs showed a favourable hyaline-like cartilage appearance when compared to the 4-week TECs. Histologically, all in vitro TECs exhibited minimally or lack cartilaginous tissue formation. The presence of high density, lacunae-encapsulated chondrocytes, distributed in cartilaginous matrices was observed in most postimplanted TECs. Coupled with positive immunoreaction against collagen type II (the cartilage-specific marker), cartilaginous properties were noticeable more in the 2-week than the 4-week post-implanted TECs.
format	Conference or Workshop Item
author	Sha’ban, Munirah Mohamad Sukri, Norhamiza Ahmad Radzi, Muhammad Aa’zamuddin Abdul Rahman, Rozlin Mohamad, Mohd Yusof Md Nazir, Noorhidayah Zulkifly, Ahmad Hafiz Hashi, Abdurezak Abdulahi
author_facet	Sha’ban, Munirah Mohamad Sukri, Norhamiza Ahmad Radzi, Muhammad Aa’zamuddin Abdul Rahman, Rozlin Mohamad, Mohd Yusof Md Nazir, Noorhidayah Zulkifly, Ahmad Hafiz Hashi, Abdurezak Abdulahi
author_sort	Sha’ban, Munirah
title	Formation of human articular cartilage from in vitro to in vivo short-term ectopic implantation model: Gross observation, histology and immunohistochemistry analysis
title_short	Formation of human articular cartilage from in vitro to in vivo short-term ectopic implantation model: Gross observation, histology and immunohistochemistry analysis
title_full	Formation of human articular cartilage from in vitro to in vivo short-term ectopic implantation model: Gross observation, histology and immunohistochemistry analysis
title_fullStr	Formation of human articular cartilage from in vitro to in vivo short-term ectopic implantation model: Gross observation, histology and immunohistochemistry analysis
title_full_unstemmed	Formation of human articular cartilage from in vitro to in vivo short-term ectopic implantation model: Gross observation, histology and immunohistochemistry analysis
title_sort	formation of human articular cartilage from in vitro to in vivo short-term ectopic implantation model: gross observation, histology and immunohistochemistry analysis
publisher	Kulliyah of Engineering, International Islamic University Malaysia
publishDate	2016
url	http://irep.iium.edu.my/51700/ http://irep.iium.edu.my/51700/ http://irep.iium.edu.my/51700/1/51700.pdf
first_indexed	2023-09-18T21:13:15Z
last_indexed	2023-09-18T21:13:15Z
_version_	1777411393746632704

Formation of human articular cartilage from in vitro to in vivo short-term ectopic implantation model: Gross observation, histology and immunohistochemistry analysis

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