Vero cell culture on magnesium doped biphasic calcium phosphate scaffolds

The bioactivity test on the performance of porous microcarriers of pure biphasic calcium phosphate (BCP) and Mg-doped BCP was conducted in spinner vessel with Vero cell lines for 90 hours. The findings revealed that all the porous microcarriers were biocompatible as the cells already attached on t...

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Main Authors:	Abd Rahim, Toibah, Sopyan, Iis, Mel , Maizirwan
Format:	Conference or Workshop Item
Language:	English
Published:	Kulliyah of Engineering, International Islamic University Malaysia 2016
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/51703/ http://irep.iium.edu.my/51703/ http://irep.iium.edu.my/51703/1/51703.pdf

id	iium-51703
recordtype	eprints
spelling	iium-517032016-08-19T03:47:48Z http://irep.iium.edu.my/51703/ Vero cell culture on magnesium doped biphasic calcium phosphate scaffolds Abd Rahim, Toibah Sopyan, Iis Mel , Maizirwan TP248.13 Biotechnology The bioactivity test on the performance of porous microcarriers of pure biphasic calcium phosphate (BCP) and Mg-doped BCP was conducted in spinner vessel with Vero cell lines for 90 hours. The findings revealed that all the porous microcarriers were biocompatible as the cells already attached on the microcarrier after 24 hours of culture. The presence of divalent cations including Mg2+ is encouraged the spreading and adhesivity of cells onto bioceramic matrices. Magnesium also believed to increase the solubility of BCP in vitro which is useful to establish cell-material interface and thus improved cell attachment and growth. Thus, higher cell attachment, growth, and migration of cell deep inside the pores were obtained on microcarriers of 10 mol% Mg-doped BCP compared to undoped BCP. The substitution of Mg into the BCP present several advantages related to metabolic cell behaviours as FESEM results showed that Mg substitution can induce a spread-like and irregular morphology which is quite different from the cell grown onto the pure BCP where the cells just remain on the surface of the scaffold, or in a localized area within the scaffold and proliferate there. This makes porous Mg-doped BCP substrate a promising candidate as a bone substitute and microcarrier. Kulliyah of Engineering, International Islamic University Malaysia 2016 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/51703/1/51703.pdf Abd Rahim, Toibah and Sopyan, Iis and Mel , Maizirwan (2016) Vero cell culture on magnesium doped biphasic calcium phosphate scaffolds. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur. http://www.iium.edu.my/icbioe/2016/index.php/78-the-4th-international-conference-on-biotechnology-engineering
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Abd Rahim, Toibah Sopyan, Iis Mel , Maizirwan Vero cell culture on magnesium doped biphasic calcium phosphate scaffolds
description	The bioactivity test on the performance of porous microcarriers of pure biphasic calcium phosphate (BCP) and Mg-doped BCP was conducted in spinner vessel with Vero cell lines for 90 hours. The findings revealed that all the porous microcarriers were biocompatible as the cells already attached on the microcarrier after 24 hours of culture. The presence of divalent cations including Mg2+ is encouraged the spreading and adhesivity of cells onto bioceramic matrices. Magnesium also believed to increase the solubility of BCP in vitro which is useful to establish cell-material interface and thus improved cell attachment and growth. Thus, higher cell attachment, growth, and migration of cell deep inside the pores were obtained on microcarriers of 10 mol% Mg-doped BCP compared to undoped BCP. The substitution of Mg into the BCP present several advantages related to metabolic cell behaviours as FESEM results showed that Mg substitution can induce a spread-like and irregular morphology which is quite different from the cell grown onto the pure BCP where the cells just remain on the surface of the scaffold, or in a localized area within the scaffold and proliferate there. This makes porous Mg-doped BCP substrate a promising candidate as a bone substitute and microcarrier.
format	Conference or Workshop Item
author	Abd Rahim, Toibah Sopyan, Iis Mel , Maizirwan
author_facet	Abd Rahim, Toibah Sopyan, Iis Mel , Maizirwan
author_sort	Abd Rahim, Toibah
title	Vero cell culture on magnesium doped biphasic calcium phosphate scaffolds
title_short	Vero cell culture on magnesium doped biphasic calcium phosphate scaffolds
title_full	Vero cell culture on magnesium doped biphasic calcium phosphate scaffolds
title_fullStr	Vero cell culture on magnesium doped biphasic calcium phosphate scaffolds
title_full_unstemmed	Vero cell culture on magnesium doped biphasic calcium phosphate scaffolds
title_sort	vero cell culture on magnesium doped biphasic calcium phosphate scaffolds
publisher	Kulliyah of Engineering, International Islamic University Malaysia
publishDate	2016
url	http://irep.iium.edu.my/51703/ http://irep.iium.edu.my/51703/ http://irep.iium.edu.my/51703/1/51703.pdf
first_indexed	2023-09-18T21:13:16Z
last_indexed	2023-09-18T21:13:16Z
_version_	1777411394048622592

Vero cell culture on magnesium doped biphasic calcium phosphate scaffolds

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