Detergent free protein extraction protocols for white adipose tissue from halal meat

Obesity is defined as an increase in the size of fat mass being a major health problem around the global and is associated with the development of type 2 diabetes mellitus. The rapidly increasing prevalence of these diseases poses a challenging healthcare burden in terms of the associated mortalit...

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Main Authors:	Azahari, Nuraniza, Khattak, Muhammad Muzaffar Ali Khan, Mohd Shukri , Nor Azwani
Format:	Conference or Workshop Item
Language:	English
Published:	Kulliyah of Engineering, International Islamic University Malaysia 2016
Subjects:	QP Physiology TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/51717/ http://irep.iium.edu.my/51717/ http://irep.iium.edu.my/51717/1/51717.pdf

id	iium-51717
recordtype	eprints
spelling	iium-517172019-09-03T02:20:14Z http://irep.iium.edu.my/51717/ Detergent free protein extraction protocols for white adipose tissue from halal meat Azahari, Nuraniza Khattak, Muhammad Muzaffar Ali Khan Mohd Shukri , Nor Azwani QP Physiology TP248.13 Biotechnology Obesity is defined as an increase in the size of fat mass being a major health problem around the global and is associated with the development of type 2 diabetes mellitus. The rapidly increasing prevalence of these diseases poses a challenging healthcare burden in terms of the associated mortality, morbidity, and economic impact. Thus, prevention and treatment of these diseases require targeting their underlying mechanism. White adipose tissues are an important endocrine tissue which not only works as a reservoir of fat but also secrete various kinds of hormones (bioactive peptides or protein). Some of them are beneficial for human health and important for the utilization of the consumed energy and some are negatively correlated to obesity and diabetes. The objective of this study was to extract the total protein from halal meat sources i.e. chicken, beef and lamb adipose tissues using detergent free protocol. Abdominal adipose tissues were removed from chicken, beef and lamb, then rinsed with ice-cold phosphate-buffered saline (PBS) solution. Delipidation of aforementioned tissue lysates was prepared by chloroform/methanol extractions modified from Bligh and Dyer. Then, protein concentration was assessed using Protein Assay Bicinchoninate Kit (Nacalai Tesque, Japan). The experiment was conducted in triplicates and the results are presented as means ± SD. Preliminary results indicate that the extraction of 10 gram subcutaneous adipose tissues from chicken, beef and lamb yielded about 0.1 gram of protein amount which was only 1 % from the total tissue mass. The protein concentration in fats from chicken, beef and lamb were 1.25 ± 0.05, 1.75 ± 0.05 & 2.53 ± 0.07 mg/ml respectively. Adipose tissue contains a relatively smaller percentage of protein by weight. Thus, the success of protein extraction protocols depends on the extraction of as much protein as possible from fat samples. The protein extraction from wasted adipose tissues in meat sources may be useful enrichment sources for health conditions. Kulliyah of Engineering, International Islamic University Malaysia 2016 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/51717/1/51717.pdf Azahari, Nuraniza and Khattak, Muhammad Muzaffar Ali Khan and Mohd Shukri , Nor Azwani (2016) Detergent free protein extraction protocols for white adipose tissue from halal meat. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur. http://www.iium.edu.my/icbioe/2016/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QP Physiology TP248.13 Biotechnology
spellingShingle	QP Physiology TP248.13 Biotechnology Azahari, Nuraniza Khattak, Muhammad Muzaffar Ali Khan Mohd Shukri , Nor Azwani Detergent free protein extraction protocols for white adipose tissue from halal meat
description	Obesity is defined as an increase in the size of fat mass being a major health problem around the global and is associated with the development of type 2 diabetes mellitus. The rapidly increasing prevalence of these diseases poses a challenging healthcare burden in terms of the associated mortality, morbidity, and economic impact. Thus, prevention and treatment of these diseases require targeting their underlying mechanism. White adipose tissues are an important endocrine tissue which not only works as a reservoir of fat but also secrete various kinds of hormones (bioactive peptides or protein). Some of them are beneficial for human health and important for the utilization of the consumed energy and some are negatively correlated to obesity and diabetes. The objective of this study was to extract the total protein from halal meat sources i.e. chicken, beef and lamb adipose tissues using detergent free protocol. Abdominal adipose tissues were removed from chicken, beef and lamb, then rinsed with ice-cold phosphate-buffered saline (PBS) solution. Delipidation of aforementioned tissue lysates was prepared by chloroform/methanol extractions modified from Bligh and Dyer. Then, protein concentration was assessed using Protein Assay Bicinchoninate Kit (Nacalai Tesque, Japan). The experiment was conducted in triplicates and the results are presented as means ± SD. Preliminary results indicate that the extraction of 10 gram subcutaneous adipose tissues from chicken, beef and lamb yielded about 0.1 gram of protein amount which was only 1 % from the total tissue mass. The protein concentration in fats from chicken, beef and lamb were 1.25 ± 0.05, 1.75 ± 0.05 & 2.53 ± 0.07 mg/ml respectively. Adipose tissue contains a relatively smaller percentage of protein by weight. Thus, the success of protein extraction protocols depends on the extraction of as much protein as possible from fat samples. The protein extraction from wasted adipose tissues in meat sources may be useful enrichment sources for health conditions.
format	Conference or Workshop Item
author	Azahari, Nuraniza Khattak, Muhammad Muzaffar Ali Khan Mohd Shukri , Nor Azwani
author_facet	Azahari, Nuraniza Khattak, Muhammad Muzaffar Ali Khan Mohd Shukri , Nor Azwani
author_sort	Azahari, Nuraniza
title	Detergent free protein extraction protocols for white adipose tissue from halal meat
title_short	Detergent free protein extraction protocols for white adipose tissue from halal meat
title_full	Detergent free protein extraction protocols for white adipose tissue from halal meat
title_fullStr	Detergent free protein extraction protocols for white adipose tissue from halal meat
title_full_unstemmed	Detergent free protein extraction protocols for white adipose tissue from halal meat
title_sort	detergent free protein extraction protocols for white adipose tissue from halal meat
publisher	Kulliyah of Engineering, International Islamic University Malaysia
publishDate	2016
url	http://irep.iium.edu.my/51717/ http://irep.iium.edu.my/51717/ http://irep.iium.edu.my/51717/1/51717.pdf
first_indexed	2023-09-18T21:13:17Z
last_indexed	2023-09-18T21:13:17Z
_version_	1777411395065741312

Detergent free protein extraction protocols for white adipose tissue from halal meat

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