Optimisation of extraction method to obtain chitosan from squid pen

The shell or skeleton of the crustaceans were made of 30–40% protein, 30–50% calcium carbonate, and 20–30% chitin. Chitosan which is derived from the chitin is a linear polymer of α (1→4)-linked 2-amino-2-deoxy- β-D-glucopyranose. Chitosan has many potential applications due to its chemical and biol...

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Main Authors:	Ab. Hadi, Hazrina, Ab Salam, Mohd Ghazali
Format:	Conference or Workshop Item
Language:	English English
Published:	2016
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/51725/ http://irep.iium.edu.my/51725/2/poster_ICIP_zali_3.pdf http://irep.iium.edu.my/51725/3/51725_Optimisation_of_extraction_method.pdf

id	iium-51725
recordtype	eprints
spelling	iium-517252018-05-23T07:55:41Z http://irep.iium.edu.my/51725/ Optimisation of extraction method to obtain chitosan from squid pen Ab. Hadi, Hazrina Ab Salam, Mohd Ghazali RS Pharmacy and materia medica The shell or skeleton of the crustaceans were made of 30–40% protein, 30–50% calcium carbonate, and 20–30% chitin. Chitosan which is derived from the chitin is a linear polymer of α (1→4)-linked 2-amino-2-deoxy- β-D-glucopyranose. Chitosan has many potential applications due to its chemical and biological properties. Thus, this study is to identify the best effective method to extract chitosan from chitin Chitosan was obtained from the squid pen by the process of decalcification, deproteination and deacetylation. The raw squid pen was treated with 1N hydrochloric acid for decalcification. Next, it was soaked with 1M sodium hydroxide for deproteination. After that the squid pen treated with 40% sodium hydroxide for deacetylation. During deacetylation process, there are different temperatures of 70oC, 90oC, 75oC and 60oC were applied to the squid pen for different batches (batch 1, batch 2, batch 3 and batch 4 respectively). Characterization parameters such as Fourier Transform Infrared (FTIR) Spectroscopy, solubility, viscosity test and pH was conducted to identify the purity and degree of deacetylation of the extracted chitosan. The percentage of yield for batch 1, batch 2, batch 3 and batch 4 was 82.30%, 82.80%, 95.60% and 37.70% respectively. For the solubility test, all the batches of chitosan slightly soluble in glycerin but shows a good solubility in different concentration of acetic acid and acetic acid+glycerin. For pH test, the chitosan that solubilized in 1% acetic acid has pH 3 while chitosan that solubilized in 0.5% acetic acid has pH of 3-4. Meanwhile for viscosity test, batch 1, batch 2, batch 3 and batch 4 have viscosity of 1144.34cP, 771.52cP, 1247.90cP and 1812.30cP respectively. For FTIR characterization, the trend of the spectrum of extracted chitosan was the same as the spectrum of standard commercialized chitosan. All the extracted chitosan has stretches the bands at 1650cm-1 and 1320cm-1. The optimum method to extract the chitosan was found to be from batch 3 which reflected by giving highest yield of chitosan. In addition, the chitosan extracted from this method also found to have the highest solubility in acidic solvent, good viscosity and pH that suitable for formulation. 2016 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/51725/2/poster_ICIP_zali_3.pdf application/pdf en http://irep.iium.edu.my/51725/3/51725_Optimisation_of_extraction_method.pdf Ab. Hadi, Hazrina and Ab Salam, Mohd Ghazali (2016) Optimisation of extraction method to obtain chitosan from squid pen. In: International Conference on Industrial Pharmacy (2nd ICIP 2016), 15th-16th Aug. 2016, Kuantan, Pahang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Ab. Hadi, Hazrina Ab Salam, Mohd Ghazali Optimisation of extraction method to obtain chitosan from squid pen
description	The shell or skeleton of the crustaceans were made of 30–40% protein, 30–50% calcium carbonate, and 20–30% chitin. Chitosan which is derived from the chitin is a linear polymer of α (1→4)-linked 2-amino-2-deoxy- β-D-glucopyranose. Chitosan has many potential applications due to its chemical and biological properties. Thus, this study is to identify the best effective method to extract chitosan from chitin Chitosan was obtained from the squid pen by the process of decalcification, deproteination and deacetylation. The raw squid pen was treated with 1N hydrochloric acid for decalcification. Next, it was soaked with 1M sodium hydroxide for deproteination. After that the squid pen treated with 40% sodium hydroxide for deacetylation. During deacetylation process, there are different temperatures of 70oC, 90oC, 75oC and 60oC were applied to the squid pen for different batches (batch 1, batch 2, batch 3 and batch 4 respectively). Characterization parameters such as Fourier Transform Infrared (FTIR) Spectroscopy, solubility, viscosity test and pH was conducted to identify the purity and degree of deacetylation of the extracted chitosan. The percentage of yield for batch 1, batch 2, batch 3 and batch 4 was 82.30%, 82.80%, 95.60% and 37.70% respectively. For the solubility test, all the batches of chitosan slightly soluble in glycerin but shows a good solubility in different concentration of acetic acid and acetic acid+glycerin. For pH test, the chitosan that solubilized in 1% acetic acid has pH 3 while chitosan that solubilized in 0.5% acetic acid has pH of 3-4. Meanwhile for viscosity test, batch 1, batch 2, batch 3 and batch 4 have viscosity of 1144.34cP, 771.52cP, 1247.90cP and 1812.30cP respectively. For FTIR characterization, the trend of the spectrum of extracted chitosan was the same as the spectrum of standard commercialized chitosan. All the extracted chitosan has stretches the bands at 1650cm-1 and 1320cm-1. The optimum method to extract the chitosan was found to be from batch 3 which reflected by giving highest yield of chitosan. In addition, the chitosan extracted from this method also found to have the highest solubility in acidic solvent, good viscosity and pH that suitable for formulation.
format	Conference or Workshop Item
author	Ab. Hadi, Hazrina Ab Salam, Mohd Ghazali
author_facet	Ab. Hadi, Hazrina Ab Salam, Mohd Ghazali
author_sort	Ab. Hadi, Hazrina
title	Optimisation of extraction method to obtain chitosan from squid pen
title_short	Optimisation of extraction method to obtain chitosan from squid pen
title_full	Optimisation of extraction method to obtain chitosan from squid pen
title_fullStr	Optimisation of extraction method to obtain chitosan from squid pen
title_full_unstemmed	Optimisation of extraction method to obtain chitosan from squid pen
title_sort	optimisation of extraction method to obtain chitosan from squid pen
publishDate	2016
url	http://irep.iium.edu.my/51725/ http://irep.iium.edu.my/51725/2/poster_ICIP_zali_3.pdf http://irep.iium.edu.my/51725/3/51725_Optimisation_of_extraction_method.pdf
first_indexed	2023-09-18T21:13:17Z
last_indexed	2023-09-18T21:13:17Z
_version_	1777411395760947200

Optimisation of extraction method to obtain chitosan from squid pen

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