Effect of media compositions on bioprotein production by mixed culture of Phanerochaete chrysosporium and Candida utilis using palm kernel cake and seaweed as substrates
In aqua-cultural production, feeding represent over 50% of the production cost of which protein constitute the most expensive dietary source. Bioprotein is a promising replacement for the commercial fish feed supplement. In this study, the enrichment of the bioprotein content after solid state fer...
| Main Authors: | , , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Kulliyah of Engineering, International Islamic University Malaysia
2016
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/51761/ http://irep.iium.edu.my/51761/ http://irep.iium.edu.my/51761/1/51761.pdf |
| Summary: | In aqua-cultural production, feeding represent over 50% of the production cost of which protein constitute
the most expensive dietary source. Bioprotein is a promising replacement for the commercial fish feed
supplement. In this study, the enrichment of the bioprotein content after solid state fermentation using palm
kernel cake and seaweed by the white rot fungus; Phanerochaete chrysoporium and yeast; Candida utilis was
investigated. The growth media components were selected from 11 types of media using Plackett-Burman
design (PBD) and were optimized by one-factor-at-a-time (OFAT) method with bioprotein concentration
(mg/g) as the response. From the screening result using PBD, three media components; K2HPO4,
CuSO4.5H2O and MnSO4.H2O were selected for further optimization using OFAT method due to their
positive contributions to the response. The final results showed that 5.0 g/L K2HPO4, 3.0 g/L CuSO4.5H2O
and 0.1 g/L MnSO4.H2O were found to be the optimum media constituents with 9.0 g/L, MgSO4.7H2O, 0.1
g/L, CaCl2.H2O, 3.0 g/L FeSO4.7H2O and 3.0 g/L peptone as fixed compositions. At this optimum
concentration, the protein increment of 11% was observed as compared to the results obtained by using PBD.
It was found that mixed cultures can improve the protein concentrations which can be used as fish feed. |
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