Biotransformation of ethyl p-methoxycinnamate and cytotoxicity activity of its biotransformed product
The extraction of Kaempferia galanga rhizome using steam distillation and supercritical fluid extraction (SFE) was carried out. After fractionation, the major compound of the K. galanga extract, ethyl p-methoxycinnamate (EPMC) was transformed using Aspergillus niger into ethyl p-hydroxycinnamate (...
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Kulliyah of Engineering, International Islamic University Malaysia
2016
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| Online Access: | http://irep.iium.edu.my/51783/ http://irep.iium.edu.my/51783/ http://irep.iium.edu.my/51783/1/51783.pdf |
| Summary: | The extraction of Kaempferia galanga rhizome using steam distillation and supercritical fluid extraction (SFE)
was carried out. After fractionation, the major compound of the K. galanga extract, ethyl p-methoxycinnamate
(EPMC) was transformed using Aspergillus niger into ethyl p-hydroxycinnamate (EPHC). The biological
anticancer activity of EPMC and its biotransformed product (EPHC) was established by cytotoxicity activity on
the human breast cancer (MCF-7) cell line using MTT assay. Ethyl p-hydroxycinnamate (EPHC) was most
cytotoxic against MCF-7 at 1000 μg/mL where percentage of cell viability was 9.87 %, while IC50 was 340 μg/mL.
EPHC showed slightly higher cytotoxicity activity compared to EPMC. The results of this study show that the
biotransformation process was able to produce a metabolite (EPHC) with higher cytotoxicity activity compared to
its parent compound (EPMC).
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