Biotransformation of ethyl p-methoxycinnamate and cytotoxicity activity of its biotransformed product

The extraction of Kaempferia galanga rhizome using steam distillation and supercritical fluid extraction (SFE) was carried out. After fractionation, the major compound of the K. galanga extract, ethyl p-methoxycinnamate (EPMC) was transformed using Aspergillus niger into ethyl p-hydroxycinnamate (...

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Main Authors:	Omar, Muhammad Nor, Mohd Hasali, Nor Hazwani, Yarmo, Mohd Ambar
Format:	Conference or Workshop Item
Language:	English
Published:	Kulliyah of Engineering, International Islamic University Malaysia 2016
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/51783/ http://irep.iium.edu.my/51783/ http://irep.iium.edu.my/51783/1/51783.pdf

id	iium-51783
recordtype	eprints
spelling	iium-517832016-08-24T03:02:03Z http://irep.iium.edu.my/51783/ Biotransformation of ethyl p-methoxycinnamate and cytotoxicity activity of its biotransformed product Omar, Muhammad Nor Mohd Hasali, Nor Hazwani Yarmo, Mohd Ambar TP248.13 Biotechnology The extraction of Kaempferia galanga rhizome using steam distillation and supercritical fluid extraction (SFE) was carried out. After fractionation, the major compound of the K. galanga extract, ethyl p-methoxycinnamate (EPMC) was transformed using Aspergillus niger into ethyl p-hydroxycinnamate (EPHC). The biological anticancer activity of EPMC and its biotransformed product (EPHC) was established by cytotoxicity activity on the human breast cancer (MCF-7) cell line using MTT assay. Ethyl p-hydroxycinnamate (EPHC) was most cytotoxic against MCF-7 at 1000 μg/mL where percentage of cell viability was 9.87 %, while IC50 was 340 μg/mL. EPHC showed slightly higher cytotoxicity activity compared to EPMC. The results of this study show that the biotransformation process was able to produce a metabolite (EPHC) with higher cytotoxicity activity compared to its parent compound (EPMC). Kulliyah of Engineering, International Islamic University Malaysia 2016 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/51783/1/51783.pdf Omar, Muhammad Nor and Mohd Hasali, Nor Hazwani and Yarmo, Mohd Ambar (2016) Biotransformation of ethyl p-methoxycinnamate and cytotoxicity activity of its biotransformed product. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur. http://www.iium.edu.my/icbioe/2016/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Omar, Muhammad Nor Mohd Hasali, Nor Hazwani Yarmo, Mohd Ambar Biotransformation of ethyl p-methoxycinnamate and cytotoxicity activity of its biotransformed product
description	The extraction of Kaempferia galanga rhizome using steam distillation and supercritical fluid extraction (SFE) was carried out. After fractionation, the major compound of the K. galanga extract, ethyl p-methoxycinnamate (EPMC) was transformed using Aspergillus niger into ethyl p-hydroxycinnamate (EPHC). The biological anticancer activity of EPMC and its biotransformed product (EPHC) was established by cytotoxicity activity on the human breast cancer (MCF-7) cell line using MTT assay. Ethyl p-hydroxycinnamate (EPHC) was most cytotoxic against MCF-7 at 1000 μg/mL where percentage of cell viability was 9.87 %, while IC50 was 340 μg/mL. EPHC showed slightly higher cytotoxicity activity compared to EPMC. The results of this study show that the biotransformation process was able to produce a metabolite (EPHC) with higher cytotoxicity activity compared to its parent compound (EPMC).
format	Conference or Workshop Item
author	Omar, Muhammad Nor Mohd Hasali, Nor Hazwani Yarmo, Mohd Ambar
author_facet	Omar, Muhammad Nor Mohd Hasali, Nor Hazwani Yarmo, Mohd Ambar
author_sort	Omar, Muhammad Nor
title	Biotransformation of ethyl p-methoxycinnamate and cytotoxicity activity of its biotransformed product
title_short	Biotransformation of ethyl p-methoxycinnamate and cytotoxicity activity of its biotransformed product
title_full	Biotransformation of ethyl p-methoxycinnamate and cytotoxicity activity of its biotransformed product
title_fullStr	Biotransformation of ethyl p-methoxycinnamate and cytotoxicity activity of its biotransformed product
title_full_unstemmed	Biotransformation of ethyl p-methoxycinnamate and cytotoxicity activity of its biotransformed product
title_sort	biotransformation of ethyl p-methoxycinnamate and cytotoxicity activity of its biotransformed product
publisher	Kulliyah of Engineering, International Islamic University Malaysia
publishDate	2016
url	http://irep.iium.edu.my/51783/ http://irep.iium.edu.my/51783/ http://irep.iium.edu.my/51783/1/51783.pdf
first_indexed	2023-09-18T21:13:24Z
last_indexed	2023-09-18T21:13:24Z
_version_	1777411402814717952

Biotransformation of ethyl p-methoxycinnamate and cytotoxicity activity of its biotransformed product

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