Temperature effect on expression of soluble Endoglucanase I from Fusarium oxysporum using E. coli Lemo21:DE3
Formation of inclusion bodies especially when expressing eukaryotic recombinant proteins in prokaryotic host such as E. coli is a challenge. Ability to express soluble proteins in E.coli will enable us to utilize all the benefits of E.coli as a host mainly the short doubling time. As few reports i...
| Main Authors: | , , , |
|---|---|
| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Kulliyah of Engineering, International Islamic University Malaysia
2016
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/51788/ http://irep.iium.edu.my/51788/ http://irep.iium.edu.my/51788/1/51788.pdf |
| Summary: | Formation of inclusion bodies especially when expressing eukaryotic recombinant proteins in prokaryotic
host such as E. coli is a challenge. Ability to express soluble proteins in E.coli will enable us to utilize all the
benefits of E.coli as a host mainly the short doubling time. As few reports indicates that temperature
influences the expression of recombinant proteins, this study aims to observe its effect on solubility of
endoglucanase I. The optimum temperature for expressing endoglucanase I from Fusarium oxysporum using
Lemo21:DE3 as a host is reported here. |
|---|