Temperature effect on expression of soluble Endoglucanase I from Fusarium oxysporum using E. coli Lemo21:DE3
Formation of inclusion bodies especially when expressing eukaryotic recombinant proteins in prokaryotic host such as E. coli is a challenge. Ability to express soluble proteins in E.coli will enable us to utilize all the benefits of E.coli as a host mainly the short doubling time. As few reports i...
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| Format: | Conference or Workshop Item |
| Language: | English |
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Kulliyah of Engineering, International Islamic University Malaysia
2016
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| Online Access: | http://irep.iium.edu.my/51788/ http://irep.iium.edu.my/51788/ http://irep.iium.edu.my/51788/1/51788.pdf |
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iium-51788 |
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eprints |
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iium-517882016-08-24T07:00:01Z http://irep.iium.edu.my/51788/ Temperature effect on expression of soluble Endoglucanase I from Fusarium oxysporum using E. coli Lemo21:DE3 Noor Batcha, Ibrahim Ali Raja Izzuddin, Raja Hawa Nurathirah Ahmad, Abdul Aziz Mohd Salleh, Hamzah TP248.13 Biotechnology Formation of inclusion bodies especially when expressing eukaryotic recombinant proteins in prokaryotic host such as E. coli is a challenge. Ability to express soluble proteins in E.coli will enable us to utilize all the benefits of E.coli as a host mainly the short doubling time. As few reports indicates that temperature influences the expression of recombinant proteins, this study aims to observe its effect on solubility of endoglucanase I. The optimum temperature for expressing endoglucanase I from Fusarium oxysporum using Lemo21:DE3 as a host is reported here. Kulliyah of Engineering, International Islamic University Malaysia 2016 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/51788/1/51788.pdf Noor Batcha, Ibrahim Ali and Raja Izzuddin, Raja Hawa Nurathirah and Ahmad, Abdul Aziz and Mohd Salleh, Hamzah (2016) Temperature effect on expression of soluble Endoglucanase I from Fusarium oxysporum using E. coli Lemo21:DE3. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur. http://www.iium.edu.my/icbioe/2016/ |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English |
| topic |
TP248.13 Biotechnology |
| spellingShingle |
TP248.13 Biotechnology Noor Batcha, Ibrahim Ali Raja Izzuddin, Raja Hawa Nurathirah Ahmad, Abdul Aziz Mohd Salleh, Hamzah Temperature effect on expression of soluble Endoglucanase I from Fusarium oxysporum using E. coli Lemo21:DE3 |
| description |
Formation of inclusion bodies especially when expressing eukaryotic recombinant proteins in prokaryotic
host such as E. coli is a challenge. Ability to express soluble proteins in E.coli will enable us to utilize all the
benefits of E.coli as a host mainly the short doubling time. As few reports indicates that temperature
influences the expression of recombinant proteins, this study aims to observe its effect on solubility of
endoglucanase I. The optimum temperature for expressing endoglucanase I from Fusarium oxysporum using
Lemo21:DE3 as a host is reported here. |
| format |
Conference or Workshop Item |
| author |
Noor Batcha, Ibrahim Ali Raja Izzuddin, Raja Hawa Nurathirah Ahmad, Abdul Aziz Mohd Salleh, Hamzah |
| author_facet |
Noor Batcha, Ibrahim Ali Raja Izzuddin, Raja Hawa Nurathirah Ahmad, Abdul Aziz Mohd Salleh, Hamzah |
| author_sort |
Noor Batcha, Ibrahim Ali |
| title |
Temperature effect on expression of soluble Endoglucanase I from
Fusarium oxysporum using E. coli Lemo21:DE3
|
| title_short |
Temperature effect on expression of soluble Endoglucanase I from
Fusarium oxysporum using E. coli Lemo21:DE3
|
| title_full |
Temperature effect on expression of soluble Endoglucanase I from
Fusarium oxysporum using E. coli Lemo21:DE3
|
| title_fullStr |
Temperature effect on expression of soluble Endoglucanase I from
Fusarium oxysporum using E. coli Lemo21:DE3
|
| title_full_unstemmed |
Temperature effect on expression of soluble Endoglucanase I from
Fusarium oxysporum using E. coli Lemo21:DE3
|
| title_sort |
temperature effect on expression of soluble endoglucanase i from
fusarium oxysporum using e. coli lemo21:de3 |
| publisher |
Kulliyah of Engineering, International Islamic University Malaysia |
| publishDate |
2016 |
| url |
http://irep.iium.edu.my/51788/ http://irep.iium.edu.my/51788/ http://irep.iium.edu.my/51788/1/51788.pdf |
| first_indexed |
2023-09-18T21:13:25Z |
| last_indexed |
2023-09-18T21:13:25Z |
| _version_ |
1777411403528798208 |