Morphological aspects of multi-nucleated Trichomonas vaginalis from cervical cancer patients in Malaysia

Trichomonas vaginalis, a flagellated protozoan parasite is commonly found in the genitourinary tract. Binary fission is the only plausible mode of reproduction shown thus far but this does not account for the rapid growth rates seen in in vitro cultures within a short period of time. We have previ...

Full description

Bibliographic Details
Main Authors:	Mat Yusof, Afzan, Kumar, Suresh
Format:	Conference or Workshop Item
Language:	English English
Published:	2013
Subjects:	Q Science (General) RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Online Access:	http://irep.iium.edu.my/52043/ http://irep.iium.edu.my/52043/ http://irep.iium.edu.my/52043/1/Morphological%20aspects%20of%20multi-nucleated%20Trichomonas%20vaginalis%20from%20cervical%20cancer%20patients%20in%20Malaysia.pdf http://irep.iium.edu.my/52043/7/52043-program.pdf

id	iium-52043
recordtype	eprints
spelling	iium-520432016-10-05T02:25:36Z http://irep.iium.edu.my/52043/ Morphological aspects of multi-nucleated Trichomonas vaginalis from cervical cancer patients in Malaysia Mat Yusof, Afzan Kumar, Suresh Q Science (General) RC0254 Neoplasms. Tumors. Oncology (including Cancer) Trichomonas vaginalis, a flagellated protozoan parasite is commonly found in the genitourinary tract. Binary fission is the only plausible mode of reproduction shown thus far but this does not account for the rapid growth rates seen in in vitro cultures within a short period of time. We have previously shown ultrastructural evidence of another asexual mode of reproduction other than binary fission seen in Trichmonas vaginalis. The present study compares the number of multi-nucleated forms isolated from cervical neoplasia (CN) and non-cervical neoplasia (NCN) patients. The present study highlights the detailed characterization of the morphological aspects of 4 CN and 4 NCN isolates by using fixed staining and fluorescent staining of nuclear content. A sequential transformation highlighting nuclear division leading to the formation of multi-nucleated parasite is shown. We observe that this mode of reproduction is not commonly seen and postulate that it could be triggered to increase progeny formation due to stress. These forms were viable when tested with trypan blue dye test. The study confirms that the parasite divides by an asexual mode of reproduction other than binary fission and the parasites isolated from cervical neoplasia showing larger numbers of nuclei accounting for higher parasite numbers in cultures. 2013 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/52043/1/Morphological%20aspects%20of%20multi-nucleated%20Trichomonas%20vaginalis%20from%20cervical%20cancer%20patients%20in%20Malaysia.pdf application/pdf en http://irep.iium.edu.my/52043/7/52043-program.pdf Mat Yusof, Afzan and Kumar, Suresh (2013) Morphological aspects of multi-nucleated Trichomonas vaginalis from cervical cancer patients in Malaysia. In: Biomicroworld2013 : V International Conference on Environmental , Industrial and Applied Microbiology, 2nd-4th October 2013, Madrid Spain. (Unpublished) http://www.biomicroworld2013.org/index.html
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General) RC0254 Neoplasms. Tumors. Oncology (including Cancer)
spellingShingle	Q Science (General) RC0254 Neoplasms. Tumors. Oncology (including Cancer) Mat Yusof, Afzan Kumar, Suresh Morphological aspects of multi-nucleated Trichomonas vaginalis from cervical cancer patients in Malaysia
description	Trichomonas vaginalis, a flagellated protozoan parasite is commonly found in the genitourinary tract. Binary fission is the only plausible mode of reproduction shown thus far but this does not account for the rapid growth rates seen in in vitro cultures within a short period of time. We have previously shown ultrastructural evidence of another asexual mode of reproduction other than binary fission seen in Trichmonas vaginalis. The present study compares the number of multi-nucleated forms isolated from cervical neoplasia (CN) and non-cervical neoplasia (NCN) patients. The present study highlights the detailed characterization of the morphological aspects of 4 CN and 4 NCN isolates by using fixed staining and fluorescent staining of nuclear content. A sequential transformation highlighting nuclear division leading to the formation of multi-nucleated parasite is shown. We observe that this mode of reproduction is not commonly seen and postulate that it could be triggered to increase progeny formation due to stress. These forms were viable when tested with trypan blue dye test. The study confirms that the parasite divides by an asexual mode of reproduction other than binary fission and the parasites isolated from cervical neoplasia showing larger numbers of nuclei accounting for higher parasite numbers in cultures.
format	Conference or Workshop Item
author	Mat Yusof, Afzan Kumar, Suresh
author_facet	Mat Yusof, Afzan Kumar, Suresh
author_sort	Mat Yusof, Afzan
title	Morphological aspects of multi-nucleated Trichomonas vaginalis from cervical cancer patients in Malaysia
title_short	Morphological aspects of multi-nucleated Trichomonas vaginalis from cervical cancer patients in Malaysia
title_full	Morphological aspects of multi-nucleated Trichomonas vaginalis from cervical cancer patients in Malaysia
title_fullStr	Morphological aspects of multi-nucleated Trichomonas vaginalis from cervical cancer patients in Malaysia
title_full_unstemmed	Morphological aspects of multi-nucleated Trichomonas vaginalis from cervical cancer patients in Malaysia
title_sort	morphological aspects of multi-nucleated trichomonas vaginalis from cervical cancer patients in malaysia
publishDate	2013
url	http://irep.iium.edu.my/52043/ http://irep.iium.edu.my/52043/ http://irep.iium.edu.my/52043/1/Morphological%20aspects%20of%20multi-nucleated%20Trichomonas%20vaginalis%20from%20cervical%20cancer%20patients%20in%20Malaysia.pdf http://irep.iium.edu.my/52043/7/52043-program.pdf
first_indexed	2023-09-18T21:13:47Z
last_indexed	2023-09-18T21:13:47Z
_version_	1777411427167895552

Morphological aspects of multi-nucleated Trichomonas vaginalis from cervical cancer patients in Malaysia

Similar Items