Identification of Cryptosporidium in goats by 18S rRNA across four districts in Terengganu, Malaysia

The research aimed to access the first distribution and genotypes of Cryptosporidium spp. using molecular tools in Malaysian goats from four farms in Terengganu. Methodology and results: A total of 454 goat faecal specimens were randomly collected from four selected farms located Kuala Terengganu,...

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Main Authors:	Barudin, Mohd Aiman, Mat Yusof, Afzan
Format:	Conference or Workshop Item
Language:	English
Published:	2016
Subjects:	Q Science (General) QR Microbiology
Online Access:	http://irep.iium.edu.my/52080/ http://irep.iium.edu.my/52080/1/Identification%20of%20Cryptosporidium%20in%20Goats%20by%2018S%20rRNA%20across%20Four%20Districts%20in%20Terengganu%2C%20Malaysia.pdf

id	iium-52080
recordtype	eprints
spelling	iium-520802016-10-05T00:59:49Z http://irep.iium.edu.my/52080/ Identification of Cryptosporidium in goats by 18S rRNA across four districts in Terengganu, Malaysia Barudin, Mohd Aiman Mat Yusof, Afzan Q Science (General) QR Microbiology The research aimed to access the first distribution and genotypes of Cryptosporidium spp. using molecular tools in Malaysian goats from four farms in Terengganu. Methodology and results: A total of 454 goat faecal specimens were randomly collected from four selected farms located Kuala Terengganu, Setiu, Marang and Besut districts. Cryptosporidium spp. positive samples from the qualitative examination using combination of modified formalin-ether concentration technique (M-FECT) and modified Ziehl-Neelsen (MZN) staining were withheld for molecular characterization using nested PCR assay targeting the 18S rRNA gene. The 18S rRNA-based PCR successfully identified the predominance of zoonotic species of Cryptosporidium parvum among all studied samples. Conclusion, significance and impact of study: The genotyping study signified that goats should be considered as one of the most important reservoirs for Cryptosporidium parvum in the studied farms. It is suggested that there is the possibility of zoonotic and anthroponotic transmissions of asymptomatic cryptosporidiosis among the goats and animal handlers. Therefore, the need of further molecular subtyping of Cryptosporidium species/genotypes together with comprehensive sanitary monitoring by goat handlers are highly recommended to minimize the transmission of this parasite. 2016 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/52080/1/Identification%20of%20Cryptosporidium%20in%20Goats%20by%2018S%20rRNA%20across%20Four%20Districts%20in%20Terengganu%2C%20Malaysia.pdf Barudin, Mohd Aiman and Mat Yusof, Afzan (2016) Identification of Cryptosporidium in goats by 18S rRNA across four districts in Terengganu, Malaysia. In: Malaysian Society for Microbiology Postgraduate Seminar 2016 (MSMPS 2016), 24 August 2016, Bangi, Selangor. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General) QR Microbiology
spellingShingle	Q Science (General) QR Microbiology Barudin, Mohd Aiman Mat Yusof, Afzan Identification of Cryptosporidium in goats by 18S rRNA across four districts in Terengganu, Malaysia
description	The research aimed to access the first distribution and genotypes of Cryptosporidium spp. using molecular tools in Malaysian goats from four farms in Terengganu. Methodology and results: A total of 454 goat faecal specimens were randomly collected from four selected farms located Kuala Terengganu, Setiu, Marang and Besut districts. Cryptosporidium spp. positive samples from the qualitative examination using combination of modified formalin-ether concentration technique (M-FECT) and modified Ziehl-Neelsen (MZN) staining were withheld for molecular characterization using nested PCR assay targeting the 18S rRNA gene. The 18S rRNA-based PCR successfully identified the predominance of zoonotic species of Cryptosporidium parvum among all studied samples. Conclusion, significance and impact of study: The genotyping study signified that goats should be considered as one of the most important reservoirs for Cryptosporidium parvum in the studied farms. It is suggested that there is the possibility of zoonotic and anthroponotic transmissions of asymptomatic cryptosporidiosis among the goats and animal handlers. Therefore, the need of further molecular subtyping of Cryptosporidium species/genotypes together with comprehensive sanitary monitoring by goat handlers are highly recommended to minimize the transmission of this parasite.
format	Conference or Workshop Item
author	Barudin, Mohd Aiman Mat Yusof, Afzan
author_facet	Barudin, Mohd Aiman Mat Yusof, Afzan
author_sort	Barudin, Mohd Aiman
title	Identification of Cryptosporidium in goats by 18S rRNA across four districts in Terengganu, Malaysia
title_short	Identification of Cryptosporidium in goats by 18S rRNA across four districts in Terengganu, Malaysia
title_full	Identification of Cryptosporidium in goats by 18S rRNA across four districts in Terengganu, Malaysia
title_fullStr	Identification of Cryptosporidium in goats by 18S rRNA across four districts in Terengganu, Malaysia
title_full_unstemmed	Identification of Cryptosporidium in goats by 18S rRNA across four districts in Terengganu, Malaysia
title_sort	identification of cryptosporidium in goats by 18s rrna across four districts in terengganu, malaysia
publishDate	2016
url	http://irep.iium.edu.my/52080/ http://irep.iium.edu.my/52080/1/Identification%20of%20Cryptosporidium%20in%20Goats%20by%2018S%20rRNA%20across%20Four%20Districts%20in%20Terengganu%2C%20Malaysia.pdf
first_indexed	2023-09-18T21:13:52Z
last_indexed	2023-09-18T21:13:52Z
_version_	1777411431613857792

Identification of Cryptosporidium in goats by 18S rRNA across four districts in Terengganu, Malaysia

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